11 results about "Chemical toxicity" patented technology

The invention provides a network science-based method for evaluating a herbicide neurodevelopmental toxicity risk. The herbicide which is widely used and is detected at high frequency in the environment is collected; acquiring a herbicide target and risk genes of neurodevelopment disorder related diseases; constructing a herbicide target network and a disease module; analyzing the neuro-developmental toxicity risk of the herbicide by network proximity; constructing an overlapping network of a herbicide target network and a disease module; screening core pathogenic genes based on multiple network topology algorithms and carrying out gene enrichment analysis; the toxicity risk is evaluated from the molecular level, and the high-risk herbicide variety is effectively discriminated. And a neuro-developmental toxic molecular mechanism of the herbicide is analyzed based on a network analysis system, so that a new framework from risk assessment to mechanism analysis is constructed for toxicity research of environmental chemicals.

The present application belongs to the technical field of biological toxicity test of chemicals, and particularly relates to a chemical toxicity test method based on a self-developed pollutant exposure system. The test method is used for simulating the toxicity of chemicals in natural water bodies by means of a self-developed pollutant exposure system, adopts zebrafish as a model organism, establishes a time-concentration linear function of the tested chemicals in a poisoning area, and calculates the toxicity test method of the minimum effect concentration of the tested chemicals by observing the abnormal behavior of zebrafish or analyzing the behavior by coupling a camera tracking technology. The test method is almost a non-destructive experiment, the experimental time is short, most of the zebrafish participating in the experiment can be restored to normal state through appropriate recovery, the time cost is greatly saved, and the test is efficient and has a low mortality rate of test organisms.

The invention discloses an environment-friendly cell preservation method, and particularly relates to the field of cell preservation, the method comprises the following steps: step 1, cells to be preserved are placed in a pretreatment solution containing a natural saccharide protective agent (3-5% w / v) and an antioxidant (0.1-0.5% w / v) for incubation, the natural saccharide protective agent is trehalose (3-5% w / v), the antioxidant is a plant extracted polyphenol compound (0.1-0.5% w / v), and the pretreatment solution is mixed with the plant extracted polyphenol compound (0.1-0.5% w / v); the incubation temperature is 4-37 DEG C, and the incubation time is 30-60 minutes. Soybean protein isolate or pea protein is adopted to replace human serum albumin, ethical dispute is avoided, the degradation period is smaller than or equal to 30 days, the biodegradable buffer solution, PLGA and sodium alginate composite liquid are adopted to be completely degraded in the natural environment for 28 days, micro-plastic pollution is avoided, meanwhile, trehalose and plant polyphenol are adopted to replace DMSO, and the chemical toxicity risk is eliminated.

The disclosure relates to formulations and methods for the in chemico testing of toxins based on a discovery that measuring a reduction in enzyme activity can be used to predict in vivo toxicity, including for example, a skin corrosion, skin irritation, eye corrosion, eye irritation, lung toxicity, liver toxicity, nervous system toxicity, developmental toxicity, acute toxicity etc. Disclosed methods are rapid, easy to perform and shelf-stable approaches for identification of toxic chemicals and materials.

The invention discloses a phage receptor binding protein host prediction method based on cross-modal multi-source transfer learning, which is characterized in that the host prediction precision is enhanced by utilizing chemical toxicity data, and the challenge of scarcity of phage sequence data is overcome. First, a shared bidirectional long short-term memory network encoder is used to process features from chemical and biological modalities, and structure and sequence information is captured. Next, domain adaptation is performed through a maximum mean difference loss function, effectively aligning chemical and biological feature representations. According to the method, knowledge migration of cross-modal data is realized, the accuracy of host prediction is remarkably improved, and particularly, the precision is improved by about 30% compared with that of a traditional model in the early stage of training. Experimental results show that the method provided by the invention can realize rapid convergence and is excellent in performance when being used for processing the problem of data scarcity, a novel efficient method is provided for host prediction in the field of biological medicines, especially in phage therapy, and the method has a wide application prospect.

The present invention relates to a knowledge base-based chemical substance toxicity prediction service provision method comprising the steps of: collecting first chemical substance data related to a target chemical substance from a first chemical substance database; collecting second chemical substance data related to the target chemical substance from a second chemical substance database that is different from the first chemical substance database; collecting specialized knowledge related to the target chemical substance from a knowledge base storing chemical substance data; generating fused data by fusing the first chemical substance data and the second chemical substance data; and fusing the fused data and the specialized knowledge. The toxicity of a chemical substance may be predicted without directly performing a test on the chemical substance.

The invention relates to the technical field of chemical toxicity assessment, and particularly discloses a chemical non-animal toxicity assessment method, device and equipment and a storage medium thereof. Environmental response coding; multi-granularity representation construction is carried out; mechanism perception characterization learning; carrying out risk controlled amplification and hierarchical prediction; performing calibration and scene aggregation; according to the method, key structures and reaction centers related to toxicology are directly extracted through mechanism fragment recognition, so that the evaluation process is established on the basis of an internal mechanism of molecular toxicity generation instead of depending on a global fingerprint similarity threshold value. Therefore, the actual reactivity and risk source of molecules can be reflected more truly. Environmental reaction codes are incorporated into the process, a parent and a transformant thereof are automatically generated and evaluated, dynamic changes of chemicals under actual exposure conditions can be simulated, and toxicity underestimation or deviation caused by neglecting metabolism / environmental action in a traditional method is avoided.

The disclosure relates to formulations and methods for the in chemico testing of toxins based on a discovery that measuring a reduction in enzyme activity can be used to predict in vivo toxicity, including for example, a skin corrosion, skin irritation, eye corrosion, eye irritation, lung toxicity, liver toxicity, nervous system toxicity, developmental toxicity, acute toxicity etc. Disclosed methods are rapid, easy to perform and shelf-stable approaches for identification of toxic chemicals and materials.

The invention relates to the technical field of immune cells, and discloses a high-activity immune cell cryopreservation method and a high-activity immune cell recovery method. The cryopreservation protective agent is prepared from the following raw materials in percentage by volume: 8 to 12 percent of dimethyl sulfoxide, 5 to 8 percent of trehalose, 5 to 10 percent of dextran 40 to 60, 2 to 4 percent of human serum albumin, 0.1 to 0.5 percent of a cell factor mixture, 3 to 6 percent of an active protective agent and the balance of a basic culture medium. According to the invention, trehalose, dextran 40-60 and an active protective agent composed of a mitochondrial targeting antioxidant SS-31, a caspase inhibitor Z-VAD-FMK and membrane stability polypeptide are introduced, and the concentration and addition mode of dimethyl sulfoxide are strictly controlled, so that the damage of a traditional cryopreservation protective agent to cells due to osmotic pressure impact and chemical toxicity can be reduced.

The invention provides a microbial antidote which can be used for relieving the toxicity of toxic and harmful chemical substances in a sewage biochemical treatment system to microorganisms and repairing damaged activated sludge. The composition comprises the following components: L-cysteine, biotin, cytokinin, compound amino acid, VB6, disodium EDTA (Ethylene Diamine Tetraacetic Acid) and a plurality of trace elements. Rich nutritional ingredients of the activated sludge repairing agent can quickly repair damaged activated sludge, quickly improve the activity and concentration of the sludge and promote balance of beneficial microorganisms in a water body, so that the resistance and tolerance of a wastewater treatment system to chemical toxic substances are enhanced, and stable and efficient operation of a sewage biochemical treatment system is guaranteed.

This invention discloses a method for preparing dried cartilaginous fish skeleton specimens, aiming to address the common technical pain points in existing cartilaginous fish skeleton specimen preparation techniques, such as easy shrinkage and deformation of cartilage tissue, narrow applicable size range, lengthy production cycle, high chemical toxicity, or high operational difficulty. This method, through optimized process design, comprises five core steps: material processing, bleaching, dehydration, shaping, and specimen treatment. Combined with safe and non-toxic conventional materials, it successfully achieves standardized preparation of dried specimens of medium to large-sized complete cartilaginous fish skeletons with a snout length of over 20 cm. This invention not only significantly shortens the production cycle, but also uses safe, non-toxic, and environmentally friendly materials such as hydrogen peroxide, ethanol, and mixed desiccants, generating no harmful waste. Furthermore, it has a wide range of applications, efficiently preparing skeleton specimens of various cartilaginous fish such as rays and sharks, and can be extended to the preparation of fish ocular bones and cartilaginous tissue specimens of reptiles and mammals. It has extremely high practical value and broad prospects for promotion in fields such as biological teaching, scientific research display, and species resource preservation.