O-type foot-and-mouth disease recombined phage vaccine of pig and construction method thereof

A construction method and phage technology, which can be applied to viruses/phages, recombinant DNA technology, microorganism-based methods, etc., can solve the problems of overlapping single topological structures, and achieve the effects of improving immunogenicity, easy cultivation and low cost.
CN102416174AInactive Publication Date: 2012-04-18JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Publication Date
2012-04-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an O-type foot-and-mouth disease recombined phage vaccine of a pig and a construction method thereof. The vaccine adopts recombined T7 phage as an antigen, and the amino acid sequence such as FMDVVP1(129-169) polypeptide of Mya98 pedigree shown in SEQIDNO:2 is expressed on the surface of the recombined T7 phage; and the VP1 (129-169) polypeptide is obtained by inserting single-copy VP1(129-169) genes into the downstream of the P10B gene of the T7 phage to carry out fusion. The invention has the following technical effects that (1) the recombined phage vaccine prepared by the T7 phage carrier can overcome the shortcomings of weaker self immunogenicity of the FMDVVP1 polypeptide and the defect of lack of T auxiliary cell epitope, effectively improve the immunogenicity and stimulate the organism to generate the higher-level antibody; and (2) since the T7 phage is easy to culture, the purification is convenient, and the invention for producing the gene engineering vaccine has multiple advantages. In the O-type foot-and-mouth disease recombined phage vaccine of the pig, the on-scale production is convenient and the cost is low.
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Description

Technical field

[0001] The invention involves T7 phage display, pig -Oh -type hoof -oral virus VP1 protein's main antigen surface restructuring and application, which belongs to the field of molecular biotechnology. Background technique

[0002] Skill disease (FMD) is an acute and severe infectious disease of occasional hoof animals, which seriously threaten domestic and foreign trade at home and abroad and even human health of animals and products.Once FMD outbreaks, compulsory measures such as blocking, isolation, and killing livestock adopted will bring major economic losses to the country or region.Therefore, FMD has been listed by the World Animal Health Organization (OIE) in the first infectious disease of Class A.The pathogen of the foot -and -mouth disease is a faire -and -mouth vaccine virus, which is a member of the small RNA virus and a member of the foot -and -mouth disease virus.The virus particles are twenty -mandarin, no capsules, 20 to 30nm in diameter, and the st...

Claims

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