Breeding method of drought resistant and cold tolerant Saccharum varieties/lines containing Erianthus fulvus Ness consanguinity
A sugarcane and sugarcane technology, applied in the field of drought-resistant and cold-resistant sugarcane varieties/lines, can solve the problem of inbreeding loss of genetic heterogeneity, poor adaptability of sugarcane varieties, vitality, adaptability, perennial roots and resistance of sugarcane varieties Problems such as reverse decline
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[0018] (1) Collection and storage of sugarcane grass pollen
[0019] From mid-August to mid-September, 2000, during the full flowering period of Canegrass, cut the spike stem (with leaves) of a single plant and place it in a well-lit room to raise the stem with clear water. Mining. Gently shake the flower spikes, collect pollen with a clean and smooth white newspaper, remove impurities such as anther wall, quickly transfer to a clean and dry petri dish, spread it flat, place it in a desiccator equipped with silica gel, and store it at 10°C Dry for 1 h, then transfer to a clean and dry 1.5mL plastic centrifuge tube, and store in a -80°C refrigerator until use.
[0020] (2) Pollination hybridization
[0021] From November to December, the sugarcane grass pollen stored at low temperature was taken out of the refrigerator and placed in a curling container. On the same day, it was carried by plane from Kunming to Hainan Sugarcane Breeding Farm (Sanya Yacheng), and immediately pla...
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