A method and application of cold resistance identification of potato resources
An identification method, a potato technology, is applied in the field of identification and analysis of crop stress resistance, which can solve the problem of few molecular markers, and achieve the effects of low cost, low requirements for analysis conditions, and easy separation and extraction
Active Publication Date: 2018-11-27
HUNAN AGRICULTURAL UNIV
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However, few molecular markers are currently available
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[0047] Materials to be identified: Feiwu Ruita, BS226, BS214, Yan2, 13*10, 41*8, Zhongshu No. 3 (verified as a common potato cultivar with weak cold resistance by the Chinese Agricultural Science Vegetable and Flower Research Institute), Xingjia number two.
[0048] Control: GS393 (the U.S. National Plant Germplasm System NPGS, collected and preserved by the Hunan Potato Engineering Technology Center, and can be distributed externally), S4, Q304, AV9; substrate potted plants , the substrate is commercial vegetable cultivation soil.
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The invention discloses a method for authenticating potato resource cold tolerance and application thereof. The method comprises the following steps: 1) respectively sampling mature leaves of potatoes to be authenticated and mature leaves of cold tolerant potatoes as control; 2) keeping the leaves in a low-temperature environment with the initial temperature set as 0 DEG C for 20-40 minutes, and sampling so as to obtain a sample 1; 3) reducing the temperature to -1 DEG C from 0 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 2; 4) reducing the temperature to minus 2 DEG C from minus 1 DEG C, keeping the temperature for 50-70 minutes, and sampling so as to obtain a sample 3; 5) measuring the content of soluble sugar of the samples 1, 2 and 3; 6) performing clustering analysis on data by using DPS analysis software, and drawing diagrams; 8) obtaining the cold tolerance of the potatoes to be authenticated according to the diagram results. The method is rapid in material treatment, simple in item analysis, large in quantity of materials treated in a single batch, capable of implementing primary cold tolerance grading on a great amount of materials within a short time, high in detection efficiency and low in detection cost.
Description
technical field [0001] The invention belongs to the technical field of identification and analysis of stress resistance of crops, and relates to a method of treating isolated potato leaves with gradient low temperature, analyzing the soluble sugar content of leaves under different treatment conditions, and taking cold-resistant materials as a comparison, and systematic clustering analysis of materials and contrasting European methods An analysis method for analyzing potato cold tolerance by distance. Background technique [0002] There are more than 300 million mu of winter fallow fields in southern my country's rice production areas, and planting potatoes in winter fallow fields is a new and effective measure to improve my country's food security. The planting area of winter crop potatoes in my country has reached more than 30 million mu. Potatoes love temperature and are afraid of cold, and the growth period of winter potato seedlings is mostly in low temperature and ra...
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IPC IPC(8): G01N33/00G01N27/06
CPCG01N27/06G01N33/0098
Inventor 秦玉芝熊兴耀刘明月胡新喜宋勇何长征潘飞谢洁丁旭
Owner HUNAN AGRICULTURAL UNIV
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