Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof

A Pseudomonas, cold-resistant technology, applied in the fields of bioengineering and environmental engineering, can solve the problems of high cost, difficult to meet the standard, long processing cycle, etc., and achieve the effect of improving the denitrification effect.

Active Publication Date: 2013-11-06
HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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Problems solved by technology

[0004] Aiming at the problems that the total N in sewage treatment in winter is difficult to reach the standard, the treatment cycle is long and the cost is high, this patent screens out the strain Pseudomonas psychrophilia Den-05 which can significantly improve the efficiency of biological denitrification under low temperature and aerobic conditions from nature, and makes it into a biological Bacterial preparations provide new ideas for realizing the standard discharge of urban sewage under low temperature conditions, and have obvious practical application value for improving the operating efficiency of sewage plants and reducing costs

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  • Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
  • Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof

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specific Embodiment approach 1

[0020] (1) Take 15mL of fresh activated sludge from Xinyigou Urban Sewage Plant in Harbin City and add it to a 100mL aerobic denitrification medium triangle flask, and place it at a temperature of 10 o C. Cultivate in a shaker with a rotating speed of 150r / min for 4-5 days to obtain the enriched bacterial liquid;

[0021] (2) Take 15mL of the enriched bacteria solution and add it to a 100mL aerobic denitrification medium conical flask, repeat the culture conditions of step (1) 3 to 4 times to obtain the acclimatized bacteria solution, and draw the growth curve;

[0022] (3) Gradual dilution of the domesticated bacteria solution, the dilution gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 , take 100 μL of domesticated bacteria solution and add it to the solid aerobic denitrification medium plate, spread it evenly by coating method, place it in an incubator with a temperature of 10°C, and cultivate it for 3~4 days to obtain a single colony;

[0023] (4) Continue to p...

specific Embodiment approach 2

[0027] Specific implementation mode 2: Add the strains into the SBR reactor after expanding the culture. method for 10 o C, centrifuge the bacterial solution at 3000×g for 5 minutes, add the bacterial cells to the reactor, and add 200 mLOD of bacterial cells per cycle 660 The bacterial cells obtained by centrifugation of the 1.0 bacterial liquid were continuously added to the bacterial cells for 4 cycles, and the NO in the reactor was measured. 3 - content. The volume of the SBR reactor is 12L, the period is 8h, the settling time is 2h, the aeration time and stirring time are 4h and 2h respectively, and the operation mode is 2h of aeration followed by 1h of stirring. Water distribution composition: every 20L distribution water contains 25.58g sodium acetate, 10g NH 4 Cl, 2g MgSO 4 ·7H 2 O, 2g CaCl 2 2H 2 O, 2g KH 2 PO 4 , 2g K 2 HPO 4 . Add a certain amount of KNO 3 , measured NO for 20 consecutive days 3 - concentration, data such as figure 2 .

[0028] The...

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Abstract

A cold-tolerant pseudomonas strain Den-05 belongs to the technical field of the biological engineering and environmental engineering, and is Pseudomonas psychrophilia Den-05, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 27, 2012 with the preservation number of CGMCC NO.5942. Low-temperature aerobic denitrifying bacteria Pseudomonas psychrophilia Den-05 is screened from the natural environment by a microbial pure culture technique, so that the preparation of a biological denitrifying bacterial agent is realized and a high-efficiency denitrifying bacterial agent is provided for a biological enhancement system; besides, the winter denitrification effect of an urban sewage plant is improved in the control and regulation way of shortening the period of aeration by shortening the anaerobic period and the removal of nitrate nitrogen under the aerobic condition is realized; as a result, the water quality discharged is capable of meeting the requirements.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering. It involves the strain Pseudomonas psychrophilia Den-05 (Pseudomonas psychrophilia Den-05) and its screening method and its application in urban sewage treatment under low temperature conditions. Background technique [0002] An appropriate amount of nitrogen in water is a basic element for synthetic algae and other microorganisms, an important material condition for the formation of an aquatic ecological environment, and the basis for a virtuous cycle of the water environment. However, at present, the total amount of nitrogen in some water bodies in my country has exceeded the capacity of the water body. Excessive nitrogen causes the abnormal proliferation of algae and other organisms in the water body, causing water quality to deteriorate. At the same time, ammonia nitrogen and nitrite nitrogen are toxic to fish and other organisms. Since the vari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/38
CPCY02W10/10
Inventor 马放庞长泷崔迪李昂邱天孔祥震蔡蕊
Owner HIT YIXING ACAD OF ENVIRONMENTAL PROTECTION
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