Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof
A Pseudomonas, cold-resistant technology, applied in the fields of bioengineering and environmental engineering, can solve the problems of high cost, difficult to meet the standard, long processing cycle, etc., and achieve the effect of improving the denitrification effect.
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Examples
specific Embodiment approach 1
[0020] (1) Take 15mL of fresh activated sludge from Xinyigou Urban Sewage Plant in Harbin City and add it to a 100mL aerobic denitrification medium triangle flask, and place it at a temperature of 10 o C. Cultivate in a shaker with a rotating speed of 150r / min for 4-5 days to obtain the enriched bacterial liquid;
[0021] (2) Take 15mL of the enriched bacteria solution and add it to a 100mL aerobic denitrification medium conical flask, repeat the culture conditions of step (1) 3 to 4 times to obtain the acclimatized bacteria solution, and draw the growth curve;
[0022] (3) Gradual dilution of the domesticated bacteria solution, the dilution gradient is 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 , take 100 μL of domesticated bacteria solution and add it to the solid aerobic denitrification medium plate, spread it evenly by coating method, place it in an incubator with a temperature of 10°C, and cultivate it for 3~4 days to obtain a single colony;
[0023] (4) Continue to p...
specific Embodiment approach 2
[0027] Specific implementation mode 2: Add the strains into the SBR reactor after expanding the culture. method for 10 o C, centrifuge the bacterial solution at 3000×g for 5 minutes, add the bacterial cells to the reactor, and add 200 mLOD of bacterial cells per cycle 660 The bacterial cells obtained by centrifugation of the 1.0 bacterial liquid were continuously added to the bacterial cells for 4 cycles, and the NO in the reactor was measured. 3 - content. The volume of the SBR reactor is 12L, the period is 8h, the settling time is 2h, the aeration time and stirring time are 4h and 2h respectively, and the operation mode is 2h of aeration followed by 1h of stirring. Water distribution composition: every 20L distribution water contains 25.58g sodium acetate, 10g NH 4 Cl, 2g MgSO 4 ·7H 2 O, 2g CaCl 2 2H 2 O, 2g KH 2 PO 4 , 2g K 2 HPO 4 . Add a certain amount of KNO 3 , measured NO for 20 consecutive days 3 - concentration, data such as figure 2 .
[0028] The...
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Description
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Application Information
- IPC
- C12N1/20; C02F3/02; C02F3/34; C12R1/38
- CPC
- Y02W10/10
- Inventors
- 马放; 庞长泷