Group of SNP molecular markers for differentiating bred sesame varieties in China
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A technology of sesame seeds and varieties, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., to achieve uniform distribution, good stability, and stable and reliable test results.
Active Publication Date: 2017-08-04
HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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However, for many domestic sesame varieties, due to the limitations of traditional identification methods, in the prior art, there is still a lack of one or a group of better targeted SNP marker molecules, so as to facilitate the identification of related sesame varieties
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Embodiment 1
[0088] This example mainly introduces the screening process for the 15 polymorphic SNP site markers described in this application. The specific process is introduced as follows.
[0089] (1) Selection of polymorphic SNP markers and primer design
[0090] The genome of Sesamum indicum L. The Sesame Genome Working Group. XXIV International Plant and Animal Genome Conference.San Diego, USA, 2016, January, 9-13.), referring to other relevant materials, selected 52 polymorphic SNP markers distributed on 13 chromosomes for the existing 151 domestic sesame varieties;
[0091] Primer pairs for amplifying the above SNP markers were designed using Primer premier 5.0 software; specific SNP primer pair information is shown in Table 3 below.
[0092] It should be explained that in order to distinguish different SNP alleles of the detected genomic DNA, the following two design principles were referred to when designing SNP primers for PCR amplification:
[0093] (1) Design three SNP prim...
Embodiment 2
[0132] In this implementation, taking Jinzhi No. 6 sesame variety as an example, the identification process of the 15 SNP polymorphic sites described in Example 1 and the fingerprint construction process are further briefly introduced as follows.
[0133] Get Jinzhi No. 6 sesame seeds, refer to the seedling cultivation process of Example 1, and extract genomic DNA, use this as a template, and use the 15 SNP polymorphism primer pairs (as shown in Table 1) screened in Example 1 to carry out PCR amplification.
[0134] The final electrophoresis pattern of Jinzhi No. 6 is as follows: figure 2 shown.
[0135] The construction process of the DNA fingerprint of Jinzhi No. 6 is as follows: According to the above-mentioned PCR results using 15 SNP markers and the SNP site marking method, the PCR amplification information of the 15 polymorphic SNP sites of Jinzhi No. 6 was combined, Namely CCAAAATCATGACCC; the SNP feature information is the DNA fingerprint of Jinzhi No. 6. The compar...
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Abstract
The invention belongs to the technical field of biological breeding and in particular relates to a group of SNP molecular markers for accurately differentiating bred sesame varieties in China. For 151 bred sesame varieties in China, SNP marker screening and developing work is performed at a targeted manner. By screening the determined 15 polymorphic SNP markers, 151 SNP finger-prints of sesame varieties in China are finally constructed successfully. Compared with an existing sesame variety identification method, the invention develops a group of polymorphic SNP markers for differentiating sesame varieties, can provide a technical support for quickly identifying and evaluating specificity of the sesame varieties and ties of consanguinity, can provide theoretical and data support for establishing a sesame molecule-assisted breeding technical platform, can provide an efficient detection method for breeding excellent novel sesame varieties and can provide a theoretical ground and technical means for work of evaluating genetic characteristics of sesame in China and identifying the sesame varieties and the like, and has relatively important theoretical value and application meaning.
Description
technical field [0001] The invention belongs to the technical field of biological breeding, and in particular relates to a group of SNP markers that can accurately distinguish Chinese bred sesame varieties. Background technique [0002] Sesame( Sesamum indicum L., 2n=26), belonging to the genus Linaceae, is an important characteristic high-quality oil crop in my country. Since the 1960s, a total of 151 new sesame varieties have been bred in my country, including 135 white sesame varieties and 16 black sesame varieties. Affected by factors such as the narrow genetic basis of sesame germplasm and the high utilization rate of a small number of excellent parents, the genetic differences among the main sesame varieties in my country are getting smaller and smaller, and the field phenotypes are relatively similar. The use of field morphological traits to distinguish and identify sesame varieties is accurate In addition, the morphological identification method has a long cycle and...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 张海洋苗红梅魏利斌李春徐芳芳段迎辉孔静静张欣童张战有
Owner HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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