Selective breeding method of male sterile line of Capsicum annuum var.frutescens L.

Abstract

The invention belongs to the technical field of breeding of vegetable crops, and discloses a selective breeding method of a male sterile line of Capsicum annuum var.frutescens L.. The selective breeding comprises the following steps: using an own common pepper maintenance line material as a female parent, selecting a single plant having a target character as a male parent from introduced three-line hybrid separation offspring having the target character and carrying out hybridization, and then carrying out inbreeding on the offspring to obtain hybridized F2; performing offspring fertility identification on a pre-selected maintenance line material (F2), selecting a plant line sterile plant of which a sterile plant ratio is 100 percent as the female parent, and using the single plant havingthe target character in the corresponding male parent plant line as the male parent and carrying out single-plant paired backcross, and simultaneously carrying out male parent inbreeding; carrying outbackcross and transformed breeding on the sterile line by utilizing a nuclear substitutional technology. The selective breeding method of the male sterile line of the Capsicum annuum var.frutescens L. disclosed by the invention is strong in complementarity of the characters and is wide in selection ranges; materials are easy to get, and the utilization rate of variety resources is improved; the method is simple, the controllability is strong and the reproduction rate is high; the bred sterile line is good in stability, high in combining ability, and is insensitive to temperature and illumination; moreover, the sterile plant rate and the sterility degree are both up to 100 percent.

Description

technical field [0001] The invention belongs to the technical field of vegetable crop breeding, and in particular relates to a method for selecting male sterile lines of capsicum capsicum. Background technique [0002] Chaotian pepper (Capsicum annuum var. frutescens L.) is a variety of pepper. It is a general term for a type of pepper that grows upwards. The pepper fruit is small, clustered or solitary, with high spiciness and easy drying. It is mainly used as dried pepper Variety utilization, is one of the three series of dried peppers in my country. Capsicum is a frequently cross-pollinated plant with greater heterosis. At present domestic capsicum hybrid seed production mainly adopts the method of artificial emasculation and pollination, and the seed production procedure is cumbersome, especially the cluster-growing capsicum, which has short internodes, clustered flowers, small fruit and large amount of flowers, and it is difficult to achieve large-scale artificial emasc...

AI Technical Summary

Problems solved by technology

In particular, research on CMS breeding of Chaotian-type peppers is still facing the dual pressure of resources and technology, and the exploration and utilization of resources are mostly limited on the basis of existing resources.

At present, there is no research report on artificially synthesizing clustered Chaotian pepper CMS by using common pepper male sterile resources and clustered Chaotian pepper three-line hybrids

[0003] In summary, the existing problems in the prior art are: the research on the selection and breeding of domestic chaotian type capsicum male sterile lines at present has problems such as insufficient resources, low utilization rate, single technical method, etc.

In general, it is difficult to find materials that meet the breeding goals and have male sterility maintenance traits in the same type of pepper, but it is relatively easy to find materials in different types of peppers

Images