Breeding method of sterile and maintainer lines of north glutinous sorghum

A technology of glutinous sorghum and maintainer lines, which is applied in the breeding field of glutinous sorghum male sterile lines and maintainer lines in the north, can solve problems such as lack of waxy sorghum varieties, and achieve the goal of saving manpower, enriching germplasm resources, and strong top-soil strength Effect

Active Publication Date: 2017-09-26
SHANXI ACADEMY OF AGRI SCI SORGHUM RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem of the lack of waxy sorghum varieties in the main production areas of sorghum in the north, the invention provides a method for breeding the sterile line of waxy sorghum in the north, and its specific steps are as follows:

Method used

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  • Breeding method of sterile and maintainer lines of north glutinous sorghum
  • Breeding method of sterile and maintainer lines of north glutinous sorghum
  • Breeding method of sterile and maintainer lines of north glutinous sorghum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Breeding waxy sorghum sterile lines and waxy sorghum maintainer lines.

[0034] Breeding waxy sorghum male sterile line (named 11476A) and waxy sorghum maintainer line (named 11476B) according to the following steps, the specific steps are as follows:

[0035] (1) The maintainer line LgB / R5M874B was used as the female parent, and the waxy sorghum maintainer line 45B was used as the male parent, and crosses were carried out. The offspring were selected from a single plant with excellent agronomic traits such as disease resistance, waxy quality, and large ear, and selfed for 7 generations to breed waxy sorghum. The maintainer line of sorghum was named 11476B.

[0036] The specific method is as follows: after 45B whole panicle, artificial emasculation, collecting LgB / R5M874B pollen and crossing it for pollination, F 1 Bagging self-breeding, harvesting 3-5 ears; F 2 5 plots were planted on behalf of each other, with a total of 30 rows and a row length of 5m. In ...

Embodiment 2

[0056] Example 2 Identification of Waxy Sorghum CMS Line 11476A and Maintainer Line 11476B

[0057] 1. Character identification

[0058] Waxy sorghum CMS line 11476A has stable fertility, a growth period of about 120 days, green seedling sheaths, dark green leaves; plant height 120-130cm, ear length 32-37cm, grain weight per ear 75-80g, thousand-grain weight 24-32g, stem Strong, long spindle-shaped, tight ear, brown shell, red grain, waxy grain, easy to thresh, 0.3% shell rate, no fluff, no awn, lodging resistance.

[0059] The waxy sorghum maintainer line 11476B has a growth period of about 120 days. Seedlings have green sheaths and dark green leaves; plant height is 120-130cm, ear length is 32-37cm, grain weight per ear is 75-80g, thousand-grain weight is 24-32g, the stem is thick and long spindle-shaped , Tight ear, brown shell, red grain, waxy grain, easy to threshing, husk rate 0.3%, no fluff, no awn, lodging resistance.

[0060] 2. Indoor identification of waxiness

...

Embodiment 3

[0083] Example 3 Analysis of Combining Ability in Seed Production of Waxy Sorghum CMS Line 11476A

[0084] The experiment adopts completely randomized block design, 3 repetitions, 6 row blocks, row length 4.5m, row spacing 0.33m, plant spacing 0.21m, plot area 4.5m×2m=9m 2 . Sowing was carried out on May 14, and field management was carried out according to field management. On October 10, samples of 10 plants in the middle two rows were randomly taken from each district, dried and threshed, and the content of protein, fat, total starch, and tannin in the grain was analyzed using a near-infrared grain analyzer. The statistical analysis results are as follows:

[0085] Relative Effect Value of General Combining Ability of Waxy Sorghum CMS Line 11476A

[0086]

[0087] It can be seen from the above table that 11476A has a high total starch combining ability, and the grain starch content of the hybrid combination is relatively high.

[0088] In order to scientifically and a...

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Abstract

The invention discloses a breeding method of sterile and maintainer lines of north glutinous sorghum. The breeding method comprises the following specific steps: 1, hybridizing by taking non-glutinous sorghum LgB/R5M874B of a maintainer line as a female parent and a glutinous sorghum maintainer line 45B as a male parent, and inbreeding generations by selecting single plants with three good characters of glutinousness, large spikes and early mature; 2, hybridizing by taking a non-glutinous sorghum sterile line A2V4A as a female parent and an F2 generation in the step one as a male parent, carrying out four-generation backcross transformation by adopting a method of carrying out stable hybridization together with backcross transformation and taking good single plants of a filial generation as a male parent, and breeding a glutinous sorghum sterile line by taking good sterility, glutinous grains and heat smut resistance as breeding standards, wherein in glutinousness identification in the step one or step two, only endosperm is cut for carrying out iodine solution reaction, so that normal sprouting of the glutinous grains is ensured, and embryo is prevented from being damaged due to length cutting of the grains. According to the breeding method disclosed by the invention, good characters of yield, quality, resistance, combining ability, appearance and the like of south and north sorghum are organically integrated, so that the sterile and maintainer lines can become basic parent lines of the north glutinous sorghum, and the germplasm resource of the glutinous sorghum is enriched.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to the breeding of sorghum varieties, in particular to a method for breeding a sterile line and a maintainer line of waxy sorghum used in the north. Background technique [0002] Sorghum is one of the major miscellaneous grain crops and one of the oldest cultivated crops in the world. It is an annual herbaceous plant, belonging to the Sorghum genus of the Poaceae Sorghum family. Because it is resistant to drought, waterlogging, salinity, barrenness and wide adaptability, it can be planted from tropical to frigid regions. Its distribution in my country is also very wide, and it is planted almost all over the country. Among them, glutinous sorghum is widely used in the production of Maotai-flavored and Luzhou-flavored liquors because its grains are characterized by strong water absorption and easy gelatinization. Moutai, Wuliangye, and Luzhou Laojiao all use glutinous sorghum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 闫凤霞柳青山张一中常建忠张晓娟梁笃周福平郭琦
Owner SHANXI ACADEMY OF AGRI SCI SORGHUM RES INST
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