A new strain of Poria cocos and its high-efficiency cultivation technique

A technology of Poria cocos and strains, which is applied in the field of selection and high-efficiency cultivation of new strains of Poria cocos, can solve the problems of decreased biological efficiency, backward cultivation technology, unsatisfactory industrial benefits, etc., and achieve reduction of harm and pollution of miscellaneous bacteria, Strong anti-miscellaneous bacterial infection ability and the effect of improving the survival rate of inoculation

Active Publication Date: 2016-08-03
HUNAN BUTIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] However, domestic and foreign studies on the breeding of new strains of Poria cocos and their high-efficiency cultivation techniques are still insufficient.
The artificial cultivation technology of Poria cocos began in the 1960s, and the Poria cocos strain "5.78" was used for meat cultivation, and the section wood, round wood or tree stump after felling pine trees were mainly used as the cultivation substrate (also known as "fungus material"). The method exists that the cultivation technology is backward, and a large amount of pine resources need to be consumed, and the strains have been propagated for many years, and the vitality of the strains has declined, and the Ling body has become smaller, and natural variation occurs, and the variety is aging and degraded, and the biological efficiency is significantly reduced (from 30 %-40% down to 10%-20%), and there are defects such as inoculation survival rate, formation rate, Poria cocos yield, Poria cocos quality, resource utilization rate are all on the low side, production cost is too high, industrial benefit is not ideal

Method used

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  • A new strain of Poria cocos and its high-efficiency cultivation technique
  • A new strain of Poria cocos and its high-efficiency cultivation technique
  • A new strain of Poria cocos and its high-efficiency cultivation technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Breeding method and character analysis of a new strain of Poria cocos (CCTCCNO: M2011072)

[0050] The breeding method of Poria cocos new strain (CCTCCNO: M2011072) of the present invention comprises the steps:

[0051] 1) Cultivation of mycelium: select wild Poria cocos with uniform and firm body, thin skin and good quality, and carry out tissue culture at 24-28°C to separate sclerotia and mycelium, wherein the tissue cultured The medium consists of 1000 ml of water, 200 grams of potatoes, 20 grams of glucose, 20 grams of agar, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 1.5 grams of vitamin B1 and 5 grams of peptone;

[0052] 2) Purification and rejuvenation of mycelium: Inoculate the separated mycelium into multi-tube culture medium, then place it in a sterile box, and cultivate it at 26-28°C for 7-10 days until the mycelium is fully grown. Select the one with the best growth condition to carry out transplantation culture, w...

Embodiment 2

[0056] Example 2 Study on the Efficiency of a New Strain of Poria Cocos (CCTCCNO: M2011072)

[0057] Promoted 523,000 bags of Poria cocos new strains (CCTCCNO: M2011072), cultivated 11.5 million bags of materials, created a total output value of 120.6086 million yuan, increased profits and taxes of 79.731 million yuan, and earned foreign exchange of 192,200 US dollars from exports. The total output value reached 116 million yuan, and generated Good ecological benefits, economic benefits and social benefits are obtained, and the results are shown in Table 1.

[0058] Table 1

[0059]

[0060]

[0061] 1. Economic benefits

[0062] Promoting 523,000 packs of Poria cocos strains (CCTCCNO: M2011072) of the present invention, its sales revenue can reach 1.046 million yuan, deducting costs of 627,600 yuan, and realizing profits and taxes of 418,400 yuan; among them, 523,000 packs of strains can plant 221,900 bags (cellar) of Poria cocos , each bag (cellar) raw material is ...

Embodiment 3

[0071] Example 3 Comparative study on high-efficiency bag material cultivation and section tree cultivation of a new strain of Poria cocos (CCTCCNO: M2011072)

[0072] The new strain of Poria cocos (CCTCCNO: M2011072) is cultivated with 100 bags of bag materials, and the average loading of each bag is 5kg, a total of 500kg; Cultivation techniques require cultivation management.

[0073] The research results of the bag material cultivation and section tree cultivation of the new Poria cocos strain (CCTCCNO: M2011072) were compared and studied. The minimum mass and maximum mass of single fresh Poria cocos, the total weight and physical and chemical cost of fresh Poria cocos harvested, etc., the results are shown in Table 3.

[0074] table 3

[0075]

[0076] As can be seen from Table 3, 100 bags of new strains of Poria cocos (CCTCCNO: M2011072) were cultivated in bags, and a total of 127 fresh Poria cocos were harvested. The minimum weight of a single Poria cocos was 0.25...

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Abstract

The invention discloses a novel tuckahoe strain (china center for type culture collection (CCTCC) M2011072) and bag-material efficient cultivation technology. The novel tuckahoe stain is applied to cultivation by adopting the bag-material efficient cultivation technology. The novel tuckahoe stain is mainly characterized in that 1) a mycelium is strong, white, obvious in clamp connection; and hypha is spread quickly in a cultivation process; 2) the tuckahoe fruits earlier, the fruit body is even and strong, the color of pulp is white or light yellow, and the skin of tuckahoe is thin and presents purplish red color; 3)the novel tuckahoe strain has the advantages of being stable inheritable characteristics, strong in anti-bacteria infection capacity, high in biological efficiency and yield, good in quality and the like; and 4) the novel tuckahoe strain remarkably improves content of effective components of amino acid, pachymaran and the like in the tuckahoe.

Description

technical field [0001] The invention relates to strain selection and cultivation technology thereof, in particular to new strain selection and high-efficiency cultivation technology of poria cocos. Background technique [0002] Poria Cocos (schw) WOLF) is a polypore fungus (Basidiomycete), sweet, light, and flat in nature. , is also one of the prescriptions of "Four Gentlemen and Eight Treasures", and its market prospect is very broad. [0003] The main component of Poria cocos is β-pachyan (also known as "Poria cocos polysaccharide"), which accounts for about 93% of the dry weight. Poria polysaccharide is an immune enhancer, which has the functions of enhancing immunity, adjuvant chemotherapy, preventing and treating tumors, and anti-tumor. It has been widely used as a new anti-cancer drug, hepatitis rehabilitation drug, and used to treat bronchial asthma and chronic bronchitis. Existing Poria health products include Poria flat tablets, Poria rolls, Poria cubes, Poria pol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G1/04
Inventor 王先有戴甲木乔明安黄定祥何黎清
Owner HUNAN BUTIAN PHARMA
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