Streptomyces Iividans and application thereof

A technology of Streptomyces lividans and strains, applied in the field of bioengineering, to achieve the effect of stable performance and abundant germplasm resources

Active Publication Date: 2015-05-20
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The types and quantities of the currently reported microbial strains producing blue pigments are far from meeting the needs of various industries for the research and development of natural blue pigments. Has important scientific research and application value

Method used

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  • Streptomyces Iividans and application thereof
  • Streptomyces Iividans and application thereof
  • Streptomyces Iividans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 strain isolation and identification

[0049] (1) Morphological identification

[0050] A blue pigment-producing strain was screened and isolated from a soil sample in a park in Ningbo. After further screening and purification, the strain was inoculated on a plate for culture. After 3 days of culture on the plate, observe the characteristics of the blue pigment-producing colony, and find that the culture characteristics of the strain on Gaoshi No. 1 solid medium are: the growth of the bacteria is slower than that of the bacteria, and colonies begin to appear after 36-48 hours of culture, and can be observed after 60-72 hours to produce blue pigment. The surface of the colony is off-white, the aerial hyphae are lush and fluffy, and the mycelium in the base is blue, which can produce soluble blue pigment (see figure 1 ).

[0051] After further optical microscope and electron microscope observation, we preliminarily judged that the strain was actinomycetes, Gra...

Embodiment 2

[0060] Embodiment 2 bacterial strain fermentation produces blue pigment

[0061] Pick colonies from the test tubes where the strains were preserved for strain activation, and culture them at 37°C for 48 hours; the formula of the activation medium is: 2% soluble starch, 0.05% NaCl, 0.05% K 2 HPO 4 , 0.05%MgSO 4 , 0.001% FeSO 4 , 2% agar.

[0062] Then pick a single colony on the activated plate and transfer it to the seed culture solution for proliferation and culture, and cultivate it at 28°C for 12 hours with a rotation speed of 200r / min; the formula of the seed culture solution is: 2% soluble starch, 0.2% beef extract, 0.05 %NaCl, 0.05%K 2 HPO 4 , 0.05%MgSO 4 , the rest is water, 0.001% FeSO 4 ·7H 2 O.

[0063] Finally, the seed liquid is inoculated into the fermentation culture liquid, and the fermentation culture is carried out. In order to obtain the maximum yield of blue pigment, a series of optimizations were carried out on the components of the fermentation m...

Embodiment 3

[0101] Example 3 Obtain the basic data of blue pigment

[0102] A series of tests were carried out on the crude preparation of the blue pigment prepared in Example 2 to obtain the basic data of the blue pigment.

[0103] (1) Solubility

[0104] Take an equal amount of blue pigment crude preparation and dissolve them in 10mL of absolute ethanol, distilled water, methanol, acetone, dimethyl sulfoxide, ethyl acetate, and petroleum ether. After standing still for 2 hours, observe the solubility and color change.

[0105] (2) UV-Vis spectrum

[0106] Dissolve the crude blue pigment preparation in water, adjust the pH value of the blue pigment aqueous solution to 3, 5, 7, 9, 11, and 13 respectively, and use a UV-2550 ultraviolet-visible spectrophotometer to measure the pH value of the blue pigment in the range of 200-800nm. The blue pigment aqueous solution is scanned to obtain the ultraviolet-visible spectrum of the blue pigment.

[0107] From tests (1) and (2), it can be seen t...

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Abstract

The invention discloses Streptomyces Iividans and application thereof. The preservation number of Streptomyces Iividans is CGMCC (China General Microbiological Culture Collection Center) No.8083. The application refers to the application of Streptomyces Iividans in producing blue pigments, and is specifically implemented through the steps of inoculating Streptomyces Iividans to a seed culture solution to carry out multiplication culture, thereby obtaining a seed solution; transferring the seed solution to a fermented culture solution to carry out fermented culture, thereby obtaining fermentation broth; separating and drying the fermentation broth so as to obtain a coarse blue pigment preparation. Compared with the prior art, the invention discloses Streptomyces Iividans capable of producing blue pigments, which enriches the germplasm resources of blue pigment producing bacteria; a blue pigment produced by Streptomyces Iividans is stable in performance and has certain bacteriostasis, and maximum OD585 is 15.4.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a strain of Streptomyces lividans and its application. Background technique [0002] Pigment refers to the substance that can color the mordant, which is widely used in production, life and scientific research. Natural pigments usually refer to pigments made from substances that exist in nature or secondary metabolites produced by cultivation methods for certain processing. Because of its safety, reliability, non-toxic side effects, natural color tone and multi-functionality, it has been widely used along with the development of food industry, pharmaceutical industry, daily chemical industry and breeding industry. [0003] The raw materials for processing natural pigments come from a wide range of sources (from animals and plants, microorganisms, minerals, etc.), and there are many kinds. As of 2004, there are about 600 kinds of records. However, the existing processed n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/06C09B61/00C12R1/465
Inventor 刘晓侠王玉洁孙诗清尤忠毓
Owner JIAXING UNIV
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