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Method for rapidly cultivating transgene maize inbred line

A technology of transgenic corn and inbred lines, applied in the fields of plant genetic improvement, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of transgenic corn that have not been tried

Active Publication Date: 2015-04-08
JILIN ACAD OF AGRI SCI +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, no attempt has been made in the breeding of transgenic maize inbred lines

Method used

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  • Method for rapidly cultivating transgene maize inbred line
  • Method for rapidly cultivating transgene maize inbred line
  • Method for rapidly cultivating transgene maize inbred line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Test materials

[0040] F1 was obtained by crossing the excellent maize inbred line Ji 201000 with the material containing the MON89034 gene. In the second growing season, the parthenogenetic induction line Nongda Gaoyu No. 3 (provided by China Agricultural University) was used as the male parent, and the F1 plants of the transgenic hybrid combination were used as the female parent for hybridization, and haploids were selected for the harvested F1 ears and grains in autumn. 226 pseudo-haploid grains were identified ( figure 1 ).

[0041] 2. Haploid doubling

[0042] Using the method of soaking buds by chemical doubling, colchicine (Col) and dimethyl sulfoxide (Dimethyl sulfoxide, DMSO) were used as the drug, and the method was the same as before. 205 pseudo-haploid seedlings were obtained and transplanted to isolated In the field, 190 quasi-haploid plants were identified through field identification, 40 ears were self-pollinated in summer, and 30 ears were harvest...

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Abstract

The invention belongs to the crop breeding field, and concreatly relates to a method for rapidly cultivating transgene maize inbred line. The method comprises the following steps: 1)operating transgene base material: performing hybridization of a transgene positive material and an excellent maize inbred line; 2)performing hybrid induction: performing hybridization of an induction line and the transgene base material; 3)performing haploid selection: selecting a haploid plant through grain and field plant; 4) doubling haploid: using a natural or an artificial chemical doubling method for doubling the haploid plant and inbreeding; and 5)transgene DH line identification: performing a PCR level detection on the obtained DH line, and confirming the transgene maize inbred line containing the target gene. The method greatly shortens a cultivation process of the transgene maize inbred line.

Description

technical field [0001] The invention belongs to the field of crop breeding, in particular to a method for rapidly cultivating transgenic corn inbred lines. Background technique [0002] As a new method of crop genetic improvement, genetic engineering and transgenic have greatly improved the quality and efficiency of breeding work. The insect-resistant transgenic corn BT176 developed by Syngenta is the world's first commercialized transgenic corn. Since the planting began in 1996, the acreage of transgenic crops has continued to increase, from 1.7 million hectares in 1996 to 175.2 million hectares in 2013. It has increased more than 100 times in 18 years, and the global market value of genetically modified seeds alone reached 15.6 billion US dollars in 2013, accounting for 35% of the global 45 billion US dollars commercial seed market. This growth has made GM technology the most rapidly adopted crop technology in the history of modern agriculture, with considerable profits. ...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68
Inventor 刘文国路明岳尧海张志军王敏刘宏伟周旭东赵万庆张建新马英杰张佩李岩王绍平
Owner JILIN ACAD OF AGRI SCI
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