Method for selectively cultivating new variety of carp

A new species, carp technology, applied in biochemical equipment and methods, fish farming, microbiological measurement/inspection, etc., can solve problems such as easy inbreeding, low probability of breeding good breeds, easy to produce inbreeding, etc. , to achieve the effects of high occurrence probability, improved economic traits and production performance, and avoid inbreeding

Inactive Publication Date: 2008-02-13
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the existing problem of inbreeding in the current carp breeding and cultivation method, which is prone to inbreeding, resulting in the decline of economic traits and production performance; the intraspecific hybridization method is large in randomness, and the probability of breeding good varieties is low, and it is also prone to occurrence Inbreeding problem, and provide a new breed of carp breeding method

Method used

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  • Method for selectively cultivating new variety of carp
  • Method for selectively cultivating new variety of carp
  • Method for selectively cultivating new variety of carp

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specific Embodiment approach 1

[0007] Specific implementation mode one: the new species carp of this embodiment is selected according to the following steps: one, select the phenotypic character according to the design requirements of the new species character, and the carp that meets the requirements is used as the breeding parent; two, detect the genetic information between the parents; three 1. Carry out breeding and grouping according to genetic information. The breeding parents are divided into at least 2 breeding groups. The genetic distance between any pair of male and female individuals in each breeding group should be greater than the genetic distance between three generations of the family; 4. Free mating and breeding within the breeding group The progeny carp is sexually mature; 5. Using the progeny carp as a new generation of breeding parents, repeat steps 1 to 4 until the progeny carp group meets the design requirements of the new variety traits, and a new variety carp is obtained.

[0008] This...

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the formula for selecting phenotypic traits in step one is: Xn = X ‾ ± 2 S , Xn in the formula is the measured value of the phenotypic traits of the parents, is the mean value of the phenotypic traits of the carp population, and S is the standard deviation of the phenotypic traits of the carp population. Other steps and parameters are the same as those in Embodiment 1.

[0012] It can be seen from the selection intensity that 1 times S means the highest selection intensity, 2 times S means the selection intensity is medium, and 3 times S means the selection intensity is the lowest. Therefore, this embodiment will be greater than the average value of phenotypic traits + twice the standard deviation and less than the average value of phenotypic traits. Carp individuals with value-d...

specific Embodiment approach 3

[0013] Specific embodiment three: the difference between this embodiment and specific embodiment one is: step 2 detects the genetic information between the parents: a. extract the breeding parent DNA; b. the breeding parent DNA is carried out PCR amplification respectively with 30 pairs of microsatellite primers Increase; c. Use map analysis software to digitize PCR amplification products; d. Import digital data into kinship analysis software for analysis and calculation to obtain genetic information. Other steps and parameters are the same as those in Embodiment 1.

[0014] In this embodiment, the breeding parents whose DNA has been extracted are to be marked, and electronic marking, conventional marking methods (cutting dorsal fins, plastic tubes of different colors and other physical marking methods) or rearing in separate pools can be used. In this embodiment, all 30 pairs of microsatellite primers may not be used, but only some of the microsatellite primers (but must be m...

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Abstract

A breeding method for the new variety of the carp relates to a breeding method of a new variety of the fish, which resolves the problems of the prior group breeding method for the culture and the cultivation of the carp that is easy to have the inbreeding, which leads to the decrease of the economic character and the production performance; the intraspecific hybridization method has great randomness, the probability for breeding an excellent variety is low and is easy to have the inbreeding. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. cultivating the filial generation to be matured; 5. adopting the offspring carp as the new generation of the breeding parent strain and repeating the operation procedures from 1 to 4 until the carp meets the character design requirements of the new variety. The present invention always uses the molecular marker to detect the genetic composition of the parent strain and the filial generation in the selection process of the new carp variety, thereby avoiding the inbreeding; moreover the character of the new variety of the carp is developed in the designed direction, the emergence probability of the excellent new variety is high; therefore, the economic character and the production performance of the carps are obviously improved.

Description

technical field [0001] The invention relates to a method for breeding new fish species. Background technique [0002] Carp is the largest aquaculture fish species in my country and even in the world. At present, carp breeding and cultivation are mainly carried out by means of group breeding, intraspecific hybridization or gynogenesis. Population selection is mainly based on phenotype selection. Before breeding, because the genetic composition of the selection object is not clear, inbreeding is easy to occur, which leads to the decline of economic traits and production performance of carp, and thus the phenomenon of economic traits decline. The randomness of intraspecies hybridization in carp is high, the probability of breeding good varieties is low, and inbreeding is also prone to occur, resulting in the decline of economic traits and production performance of carp. Contents of the invention [0003] The purpose of the present invention is to solve the existing problem o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 孙效文鲁翠云匡友谊梁利群曹顶臣
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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