Molecular marker-assisted breeding method for new rapid growth variety of trachinotus ovatus
A molecular marker-assisted, oval pomfret technology, applied in biochemical equipment and methods, microbial determination/inspection, fish farming, etc., can solve the problems of low survival rate, slow seed growth, germplasm degradation, etc. Achieve the effect of avoiding low survival rate and slow growth of seedlings
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Embodiment 1
[0040] The molecular marker-assisted breeding method of the fast-growing new species of pomfret provided by the present embodiment comprises the following steps:
[0041] (1) Promoting maturation, oxytocin and seedling cultivation of the parents, the specific steps are as follows:
[0042] Two months before the breeding season, parental reproductive regulation of the breeding population of Pompano ovata was carried out. The parent fish bait (made of a mixture of chilled squid, miscellaneous fish, skipjack tuna and multivitamins, etc., conventional bait) is used to promote the development of the brood fish gonads, and human chorionic gonadotropin (HCG) and ovulation induction are used. Oxytocin No. 2 (LRH-A2), ovulation-stimulating hormone No. 3 (LRH-A3) and 0.9% sodium chloride solution were used to prepare oxytocin reagents in proportion, and the doses of the three oxytocin hormones were 1000IU / kg, 1000IU / kg, 10mg / kg and 10mg / kg. After the broodstock is injected with the ox...
Embodiment 2
[0065] The specific steps of breeding effect evaluation and subbreeding implementation are as follows:
[0066] Refined broodstock culture was carried out on 500 retained seed individuals, and after they had grown to a certain size, gonadal maturation, induced production and seed cultivation were carried out to obtain selected seedlings. At the same time, unselected commercial seedlings of similar age and specifications were collected as a control group to carry out comparative tests of productivity in sea areas. After 6 months of breeding in the sea area, 30 fish were randomly selected from the breeding group and the control group to measure their body weight. The body weight data of the two groups of fish are shown in Table 3. The comparison test results show that the average fish body weight of the breeding group is The average body weight of the control group was 554g, and the average weight of the control group was 464g. The body weight of the selected seedlings increased...
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