Molecular marker-assisted breeding method for new rapid growth variety of trachinotus ovatus

A molecular marker-assisted, oval pomfret technology, applied in biochemical equipment and methods, microbial determination/inspection, fish farming, etc., can solve the problems of low survival rate, slow seed growth, germplasm degradation, etc. Achieve the effect of avoiding low survival rate and slow growth of seedlings

Active Publication Date: 2021-12-31
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a molecular marker-assisted breeding method for the rapid growth of new species of pomfret ovata, which can effectively solve the existing genetic breeding work of pomfret ovata Difficulties in genetic evaluation and selection of candidate parents, blind inbreeding with unclear kinship and sex lead to slow growth of seedlings, low survival rate, poor resistance to stress and disease, and germplasm degradation

Method used

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  • Molecular marker-assisted breeding method for new rapid growth variety of trachinotus ovatus
  • Molecular marker-assisted breeding method for new rapid growth variety of trachinotus ovatus
  • Molecular marker-assisted breeding method for new rapid growth variety of trachinotus ovatus

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Effect test

Embodiment 1

[0040] The molecular marker-assisted breeding method of the fast-growing new species of pomfret provided by the present embodiment comprises the following steps:

[0041] (1) Promoting maturation, oxytocin and seedling cultivation of the parents, the specific steps are as follows:

[0042] Two months before the breeding season, parental reproductive regulation of the breeding population of Pompano ovata was carried out. The parent fish bait (made of a mixture of chilled squid, miscellaneous fish, skipjack tuna and multivitamins, etc., conventional bait) is used to promote the development of the brood fish gonads, and human chorionic gonadotropin (HCG) and ovulation induction are used. Oxytocin No. 2 (LRH-A2), ovulation-stimulating hormone No. 3 (LRH-A3) and 0.9% sodium chloride solution were used to prepare oxytocin reagents in proportion, and the doses of the three oxytocin hormones were 1000IU / kg, 1000IU / kg, 10mg / kg and 10mg / kg. After the broodstock is injected with the ox...

Embodiment 2

[0065] The specific steps of breeding effect evaluation and subbreeding implementation are as follows:

[0066] Refined broodstock culture was carried out on 500 retained seed individuals, and after they had grown to a certain size, gonadal maturation, induced production and seed cultivation were carried out to obtain selected seedlings. At the same time, unselected commercial seedlings of similar age and specifications were collected as a control group to carry out comparative tests of productivity in sea areas. After 6 months of breeding in the sea area, 30 fish were randomly selected from the breeding group and the control group to measure their body weight. The body weight data of the two groups of fish are shown in Table 3. The comparison test results show that the average fish body weight of the breeding group is The average body weight of the control group was 554g, and the average weight of the control group was 464g. The body weight of the selected seedlings increased...

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Abstract

The invention discloses a molecular marker-assisted breeding method of a new variety for rapid growth of trachinotus ovatus. According to the method, by efficiently integrating technologies such as individual phenotype value selection, microsatellite molecular marker, additive genetic correlation matrix, linear hybrid model, quantity genetics, individual breeding value selection, gender specific molecular marker, inter-individual genetic distance analysis, group mating scheme and the like, the problems of slow fry growth, low survival rate, poor stress resistance and disease resistance, germplasm degeneration and the like caused by difficulty in candidate parent genetic evaluation and optimal selection and blind inbreeding due to unclear genetic relationship and sex in existing trachinotus ovatus genetic breeding work can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of aquatic animal breeding, and in particular relates to a molecular marker-assisted breeding method for a fast-growing new species of pomfret. Background technique [0002] Oval pomfret (Trachinotusovatus), commonly known as golden pomfret, is mainly distributed in the Indian Ocean, Indonesia, Australia, Japan, tropical and temperate Atlantic Ocean, the Mediterranean Sea and offshore islands in the Americas, the west coast of Africa, and the South China Sea, East China Sea, Yellow Sea, Bohai Sea and other sea areas. Oval pomfret has a bright body color, no thorns in the fish, tender meat, delicious taste, rich nutrition, suitable for all ages, and is more and more recognized and loved by consumers. In 2020, the aquaculture output of ovate pomfret in my country is estimated to have exceeded 150,000 tons, and the output value of the entire industry is nearly 10 billion yuan. It has become one of the important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10C12Q1/6879C12Q1/6888
CPCA01K61/10C12Q1/6879C12Q1/6888C12Q2600/156Y02A40/81
Inventor 刘宝锁张殿昌郭梁郭华阳朱克诚张楠
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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