2 results about "Ribosomal protein E-L30" patented technology

The application provides a preparation method of 30S ribosomal protein S1 and use of the 30S ribosomal protein S1 as an antibacterial synergist, and belongs to the technical field of biological medicines. The 30S ribosomal protein S1 in the application is a bioactive protein produced by Acinetobacter bacteria, and has the effect of enhancing the antibacterial capacity of antibiotics. In the process of being combined with streptomycin sulfate, the 30S ribosomal protein S1 greatly enhances the inhibiting capacity of streptomycin sulfate on avian pathogenic E.coli without showing the effect of inhibiting bacteria itself, which lays a foundation for future practical application, and also plays a reference role for the development of new antibacterial synergists.

The application belongs to the technical field of genetic engineering, and particularly relates to a ribosomal protein N' mutant and application thereof. The ribosomal protein N' mutant is subjected to Y190D mutation. When the ribosomal protein N' expressed by a production strain is subjected to Y190D mutation, the production of LNT II is increased by 28.6%, the production of LNnT is increased by 50%, the production of LNT is increased by 92.3%, the production of 3'-SL is increased by 13.8%, and the production of 6'-SL is increased by 20%. It can be seen that after the 190th amino acid of PriA is changed from tyrosine to aspartic acid, the production capacity is improved. When the ribosomal protein N' mutant is applied to an Escherichia coli for producing human milk oligosaccharides, the production of human milk oligosaccharides is significantly improved.