12results about How to "Improve translation efficiency" patented technology

This application relates to the field of biomedical technology, specifically disclosing a highly liquid-stable SUMO fusion influenza B NP antigen and its preparation method. The antigen is a fusion protein of a SUMO-tagged peptide and an influenza B virus nucleoprotein NP. The SUMO-tagged peptide is directly linked to the N-terminus of the influenza B virus nucleoprotein NP via peptide bonds. The antigen is dissolved in a storage buffer for liquid preservation. The preparation method includes key steps such as fusion gene construction, vector engineering construction, host transformation screening, induced expression culture, and bacterial cell disruption and purification. This antigen can be used for clinical detection of influenza B virus, preparation of quality control materials, and immunogen development. It exhibits excellent liquid preservation stability, maintaining good immunoreactivity even after long-term storage under low-temperature liquid conditions. The preparation method of this application is simple and controllable, requiring no inclusion body refolding step, and can efficiently obtain high-purity and homogeneous soluble fusion antigen.

The embodiment of the invention discloses a file conversion method and device. The specific embodiment of the method comprises the following steps: detecting the validity of a text file and a list file in response to a received request for converting the text file into the list file; in response to the detection that the text file and the list file are valid, detecting whether the original text content of the original text column of the list file is empty or not; in response to detection that the original text content of the original text column of the list file is not empty, matching the original text content of the original text column of the list file with the original text content of the original text item of the text file; and in response to successful matching, assigning the translation content of the translation item corresponding to the original text content of the original text item of the text file to the translation column of the current row where the original text content of the original text item of the list file is located. According to the embodiment, after the text file is converted into the list file, the data of the list file are clearer in row and column, and the readability is higher. And the content can be paged to facilitate item summarization and statistics. And after the list file is converted into the text file, the error rate is low, and the problems of copying serialization and the like are avoided.

This invention discloses a 5'UTR sequence and its application in improving mRNA translation efficiency. This invention belongs to the field of biotechnology. The 5'UTR sequence designed in this invention has excellent function in promoting mRNA expression. Compared with the commonly used HBA1 gene 5'UTR, the expression level of FLuc mRNA fused with the 5'UTR in this invention can be increased by 2 to 3.5 times.

The invention belongs to the technical field of in-vitro transcription, and particularly discloses a 5 'UTR element with a protein expression enhancing function, an expression vector and application. The 5 'UTR is selected from any one of an SPP1d element, a TSPAN1u1 element and an SPP1d-TSPAN1u1 series element; the nucleotide sequence of the SPP1d element is as shown in SEQ ID NO: 1, the nucleotide sequence of the TSPAN1u1 element is as shown in SEQ ID NO: 2, and the nucleotide sequence of the SPP1d-TSPAN1u1 tandem element is as shown in SEQ ID NO: 3. The 5 'UTR element can significantly improve translation efficiency, has a wide enhancement effect on functional protein expression, is high in biological safety, and provides a standardized and high-performance translation regulation tool for the fields of efficient recombinant protein production, gene therapy vector optimization, vaccine research and development and the like.

The application relates to the field of audio and video real-time translation technology and discloses an audio and video real-time intelligent shunting translation method and system combined with a buffering strategy, which comprises the following steps: analyzing real-time acquired audio and video data, shunting the audio and video data through a preset data shunting model to obtain audio data and video data; configuring a dynamic buffering mechanism, performing semantic analysis and data division on the audio data of an audio buffering area to obtain an audio data block set; analyzing the semantic importance of the data to calculate a corresponding priority, performing key frame division on the video data to obtain a video key frame priority and an audio block priority; respectively translating the video key frame and the audio data block through a preset translation model to obtain a first translation result and a second translation result; and dynamically adjusting the display time of the translation result to obtain a real-time translation result; through dynamic adjustment of the buffering area size and the hierarchical structure, the application can reduce the waiting time and improve the accuracy and real-time performance of the translation result.

The invention relates to the technical field of computers, and particularly provides an address mapping table updating method and device, equipment, a medium and a program product. The method comprises the following steps: in response to a received first write-in request, determining a first logic address to be written in first data in the first write-in request; determining a first physical address corresponding to the first logic address according to a multi-level address mapping table; writing first data according to the first physical address; and in response to successful writing of the first data, updating a root-level address mapping table in the multi-level address mapping table according to the first logic address, the first physical address and the adjacent idle physical address field, so as to add mapping information for mapping to the adjacent idle physical address field in the root-level address mapping table. In this way, the address mapping table is optimized, and then the data writing efficiency can be improved.

The embodiment of the invention provides a data processing method and device, equipment, a medium and a product. The method comprises the steps that a target interface is displayed, and at least part of to-be-processed data is displayed on the target interface; and in response to a data entry request for the target interface, determining translation associated data according to the to-be-processed data, and entering the translation associated data into the data record document. According to the technical scheme of the embodiment of the invention, the to-be-processed data of the display target interface can be automatically analyzed and processed to obtain the translation associated data, and the translation associated data is further automatically input into the data recording document; the technical problems that data entry is prone to errors and low in efficiency due to the fact that translation documents are manually filled in in related technologies are solved, manual operation is effectively reduced, the error probability of data entry operation is reduced, the data recording documents containing the translation associated data can be rapidly generated, and the data recording efficiency is improved. And the efficiency and accuracy of data translation are greatly improved.

This invention discloses a 5'UTR sequence and its application in improving mRNA translation efficiency. This invention belongs to the field of biotechnology. The 5'UTR sequence designed in this invention has excellent function in promoting mRNA expression. Compared with the commonly used HBA1 gene 5'UTR, the expression level of FLuc mRNA fused with the 5'UTR in this invention can be increased by 2 to 3.5 times.

This disclosure relates to the field of biotechnology, specifically to a 5'UTR sequence for promoting mRNA translation and its uses. This disclosure provides a 5'UTR sequence comprising the nucleotide sequence shown in any one of SEQ ID NO: 1-6 or its complementary sequence, or a nucleotide sequence having at least 80% homology with the nucleotide sequence shown in any one of SEQ ID NO: 1-6 or its complementary sequence. This disclosure provides a novel RNA molecule design scheme that, through rational optimization of the nucleic acid sequence of functional regions, achieves the advantage of optimized translation, making this RNA molecule promising for broad applications in gene therapy, vaccine development, and other fields.

The application discloses a Bantu language geographical name machine translation method and related device, and relates to the field of language translation. The method comprises the following steps: adopting a forward maximum matching algorithm and a reverse maximum matching algorithm to divide a Bantu language geographical name word group to be translated into letter combinations, and respectively calculating mutual information values of the obtained corresponding forward letter combination division result and reverse letter combination division result; determining an intermediate division result according to the comparison result of the mutual information values, and using a Bantu language Chinese translation and writing table to transliterate the intermediate division result to obtain a transliteration result. The application cuts the word group to be translated, obtains the intermediate division result which can be directly compared with the Bantu language Chinese translation and writing table for accurate proper name transliteration, and has high correlation between adjacent word groups, thereby solving the problem of proper name transliteration in Bantu language geographical name translation, enabling the machine translation model to independently perform Bantu language geographical name translation, reducing the human consumption during geographical name generation, and improving the machine translation efficiency.

The application discloses a kind of high-efficiency synthesis lactalbumin recombinant Pichia pastoris and its construction method and application, belong to biotechnology field.Original gene of lactalbumin is first doublet codon optimization, and optimization gene is obtained;Then construct the multicopy expression vector containing at least 6 copies of optimization gene and at least one high-yield auxiliary gene, by Cre-LoxP recombination technology and co-transformation, and the positive recombinant Pichia pastoris integrated with multicopy optimization gene and high-yield auxiliary gene and genetically stable is screened.The recombinant Pichia pastoris is fermented by high-density culture and methanol induction, and the expression amount of lactalbumin can reach 3.8 g / L or more, and the purity is higher than 95%.The synthesis efficiency and product activity of lactalbumin are significantly improved by the application, and are suitable for large-scale production in infant formula, health products and biomedicine field.