Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins

a technology of trichothecene and toxins, which is applied in the field of transgenic plants expressing l3 delta proteins that are resistant to trichothecene fungal toxins, can solve the problems of reducing economic value, higher cost of goods to ultimate purchasers, and pap, however, has not shown antifungal activity in vitro, so as to reduce toxicity

Inactive Publication Date: 2006-01-05
RUTGERS THE STATE UNIV
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Benefits of technology

[0022] A further aspect of the present invention is directed to a method of reducing toxicity associated with a ribosome inactivating protein (RIP) that targets a eucaryotic ribosomal L3 protein, in an animal in need thereof, comprising administering to an animal in need thereof, a composition comprising an effective amount of a polypeptide having at least the first 21 to about 99 N-terminal amino acid residues of a full-length eucaryotic L3 protein, or an analog thereof. In some embodi...

Problems solved by technology

Many commercially valuable agricultural crops are prone to infection by plant viruses and fungi capable of inflicting significant damage to a crop in a given season, and drastically reducing its economic value.
The reduct...

Method used

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  • Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins
  • Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins
  • Transgenic plants expressing L3 delta proteins are resistant to trichothecene fungal toxins

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Embodiment Construction

[0046] A primary aspect of the present invention is directed to DNA sequence that encodes a polypeptide having at least the first 21 to about 99 N-terminal amino acid residues of a full-length eucaryotic L3 protein (hereinafter “L3 N-terminal polypeptides”, or “L3 N-terminal polypeptide fragments,” or an analog of the L3 polypeptide. Eucaryotic L3 proteins include, but are not limited to human, yeast, bovine, mice, rat and higher plant (e.g., rice wheat, barley, and tobacco) and Arabidopsis L3 proteins. An alignment of the amino acid sequences of full-length L3 proteins from Arabidopsis (i.e., AtRPL3A and AthRPL3B), Nicotiana tabacum (i.e., NtRPL3-8d and NtRPL3-10d), yeast (i.e., YRPL3), and rice (i.e., HvRPL3) various L3 proteins, and their first 100 amino acid residues, are illustrated in FIGS. 11A and B. Nucleotide and corresponding amino acids sequences of the yeast wild-type L3 gene (rpl3), the tobacco “8d” L3 and “10d) proteins, and the mutant tcml gene, are illustrated in FIG...

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Abstract

Disclosed are specific mutants of L3 and transgenic plants that produce them. The plants exhibit increased resistance to fungal toxins that target ribosomal L3 protein. Also disclosed are transgenic plants that co-produce L3 mutant and an RIP protein, and exhibit increased resistance to various fungal toxins and viruses, while reducing toxicity normally associated with production of the RIP. Uses of the L3 mutants in animals are further disclosed.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application claims the benefit of the filing date of U.S. Provisional Patent Application No. 60 / 529,348 filed Dec. 12, 2003, the disclosure of which is hereby incorporated herein by reference.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH [0002] The development of this invention was supported in part by the United States Department of Agriculture grant USDA-RS-58-5325-758. Therefore, the Government may have certain rights in the invention.BACKGROUND OF THE INVENTION [0003] The subject of plant protection against pathogens remains the area of utmost importance in agriculture. Many commercially valuable agricultural crops are prone to infection by plant viruses and fungi capable of inflicting significant damage to a crop in a given season, and drastically reducing its economic value. The reduction in economic value to the farmer in turn results in a higher cost of goods to ultimate purchasers. [0004] Fungal pathogens contribute sig...

Claims

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Application Information

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IPC IPC(8): A01H1/00C12N15/82
CPCC12N15/8282
Inventor TUMER, NILGUNDI, RONG
Owner RUTGERS THE STATE UNIV
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