Molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of molecular marker

A colorectal cancer, application technology, applied in the field of tumor biodiagnosis and treatment, can solve the problem that there is no colorectal cancer metastasis molecular marker, etc.

Inactive Publication Date: 2017-08-29
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to detect specific gene products associated with disease states such as colonic carcinoma or other diseases by comparing their DNA/protein expressions against healthy controls from individuals who have had previous medical conditions like those caused by these genes. By analyzing this data over time it will help predict how well certain people are likely to develop them during future stages of illness.

Problems solved by technology

This patented technical problem addressed in this patents relates to identifying specific changes caused or associated with increased levels of certain factors involved during colorection development for improved diagnoses and treatments.

Method used

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  • Molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of molecular marker
  • Molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of molecular marker
  • Molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of molecular marker

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Embodiment 1

[0021] Example 1 Analyzing the mRNA expression level of RPL22L1 in clinical colorectal cancer samples through the database

[0022] The mRNA expression level of RPL22L1 in the expression profiling chip of clinical colorectal cancer samples from GEO and TCGA database was analyzed by Oncomine database. The result is as figure 1 shown, from figure 1 The results showed that the mRNA expression level of RPL22L1 in clinical colorectal cancer samples was significantly higher than that in paracancerous tissues.

Embodiment 2

[0023] The detection of embodiment 2 clinical sample

[0024] 1. Sample

[0025] 10 pairs of clinically matched colorectal cancer (1-10T) and adjacent (1-10N) tissues and pathological sections.

[0026] 2. Method

[0027] 2.1 Detection of RPL22L1 mRNA level in clinical colorectal cancer samples

[0028] 2.1.1 Extraction of RNA from colorectal cancer tissue

[0029] (1) Take a 2ml Eppendorf tube, add 100mg frozen tissue, 100μl 0.5mm zirconium silicate beads, 1ml Trizol.

[0030] (2) Put it in a tissue breaker, and break it at the maximum speed for 5 minutes.

[0031] (3) Add 0.2 mL of chloroform, shake vigorously for 30 s, and let stand on ice for 3 to 5 min.

[0032] (4) Centrifuge at 4°C, 12 000g, 15min.

[0033] (5) Transfer the upper aqueous phase to a new Eppendorf tube, add an equal volume of isopropanol to the supernatant, mix gently, and place at room temperature for 10 minutes.

[0034] (6) Centrifuge, 4°C, 12 000g, 15min.

[0035] (7) Discard the supernatant, ...

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Abstract

The invention discloses a molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of the molecular marker. The molecular marker is a ribosomal protein analog RPL22L1 (Ribosomal protein L22-like 1). A research proves that the ribosomal protein analog RPL22L1 is associated with occurrence and development of tumors, expression levels of the ribosomal protein analog in clinical colorectal carcinoma tissues rise, the expression levels of the ribosomal protein analog are obviously associated with metastasis occurrence and poor prognosis, and the colorectal carcinoma metastasis can be promoted by multiple regulation pathways. According to the molecular marker associated with colorectal carcinoma metastasis and prognosis disclosed by the invention, by detecting the expression levels of RPL22L1 genes in the colorectal carcinoma, the RPL22L1 as a tumor molecular diagnosis marker is applied to clinical diagnosis of the colorectal carcinoma metastasis and prognosis evaluation, so that a new molecular marker is provided for early diagnosis on the colorectal carcinoma metastasis and prognosis evaluation of colorectal carcinoma patients.

Description

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Claims

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Application Information

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Owner HARBIN MEDICAL UNIVERSITY
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