Application of antisense nucleotide sequences of ribosomal protein analogues RPL22L1 in preparing medicines capable of suppressing growth of ovarian cancer cells
A technology of RPL22L1 and ribosomal protein, applied in the direction of drug combination, introduction of foreign genetic material by carrier, anti-tumor drug, etc., can solve the problems of poor chemotherapy effect of ovarian cancer, improve specificity, reduce malignancy, and inhibit growth Effect
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Embodiment 1
[0056] The construction of embodiment 1 pSuper-shRNA-RPL22L1 gene eukaryotic expression vector
[0057] (1) Synthesize the following oligonucleotide sequences (oligos) containing the antisense nucleotide sequence of the ribosomal protein analogue RPL22L1:
[0058] hRPL22L1-SiR(333)+:GATCCCCAAGAAATACCTTAAGAAGAACTTCAAGAGAGTTTCTTCTTAAGGTATTTCTTTTTTTA (shown in SEQ ID NO.4)
[0059] hRPL22L1-SiR(333)-:AGCTTAAAAAAAGAAATACCTTAAGAAGAACTCTCTTGAAGTTTCTTCTTAAGGTATTTCTTGGG (shown in SEQ ID NO.5)
[0060] or
[0061] hRPL22L1-SiR(687)+:GATCCCCAAAATCTTTTATGTACTCAGGTTCAAGAGACCTGAGTACATAAAAAGATTTTTTTTTA (shown in SEQ ID NO.6)
[0062] hRPL22L1-SiR(687)-:AGCTTAAAAAAAAATCTTTTATGTACTCAGGTCTCTTGAACCTGAGTACATAAAAAGATTTT GGG (shown in SEQ ID NO.7)
[0063] or
[0064] hRPL22L1-SiR(926)+:GATCCCCAAAGTGCTGATTATAGCTGTGTTCAAGAGACACAGCTATAATCAGCACTTTTTTTTA (shown in SEQ ID NO.8)
[0065] hRPL22L1-SiR(926)-:AGCTTAAAAAAAAGTGCTGATTATAGCTGTGTCTCTTGAACACAGCTATAATCAGCACTTT GGG (shown in SEQ ID NO.9)
...
Embodiment 2
[0088] Example 2 Effect of Inhibiting the Expression of RPL22L1 on the Biological Behavior of UACC-1598 Cells
[0089] 1. Detection of RPL22L1 amplification in tumor cells
[0090] (1) Conventional cell culture: the human ovarian cancer cell line UACC-1598 containing double microsomes was selected as the research object, and RPMI-1640 medium containing 10% fetal bovine serum was used in CO 2 Cultured in an incubator at 37°C.
[0091] (2) Detection of RPL22L1 amplification in UACC-1598 cells by DNA and mRNA levels:
[0092] DNA level, using normal human peripheral blood DNA and human embryonic kidney cell HEK293DNA as a control, to detect the amplification of RPL22L1 in UACC-1598; Amplification in UACC-1598. The controls used in the above two levels are the samples that have been extracted in our laboratory earlier.
[0093] The DNA was extracted according to the QIAamp DNA Extract Kit (QIAGEN) instructions, and the NCBIGene database was searched to obtain the RPL22L1 gene ...
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