Application of antisense nucleotide sequences of ribosomal protein analogues RPL22L1 in preparing medicines capable of suppressing growth of ovarian cancer cells
A technology of RPL22L1 and ribosomal protein, which is applied in the direction of drug combination, the use of carriers to introduce foreign genetic material, anti-tumor drugs, etc., can solve the problems of poor chemotherapy effect of ovarian cancer and other problems, so as to improve specificity, inhibit growth and reduce the degree of malignancy Effect
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Embodiment 1
[0056] The construction of embodiment 1 pSuper-shRNA-RPL22L1 gene eukaryotic expression vector
[0057] (1) Synthesize the following oligonucleotide sequences (oligos) containing the antisense nucleotide sequence of the ribosomal protein analogue RPL22L1:
[0058] hRPL22L1-SiR(333)+:GATCCCCAAGAAATACCTTAAGAAGAACTTCAAGAGAGTTTCTTCTTAAGGTATTTCTTTTTTTA (shown in SEQ ID NO.4)
[0059] hRPL22L1-SiR(333)-:AGCTTAAAAAAAGAAATACCTTAAGAAGAACTCTCTTGAAGTTTCTTCTTAAGGTATTTCTTGGG (shown in SEQ ID NO.5)
[0060] or
[0061] hRPL22L1-SiR(687)+:GATCCCCAAAATCTTTTATGTACTCAGGTTCAAGAGACCTGAGTACATAAAAAGATTTTTTTTTA (shown in SEQ ID NO.6)
[0062] hRPL22L1-SiR(687)-:AGCTTAAAAAAAAATCTTTTATGTACTCAGGTCTCTTGAACCTGAGTACATAAAAAGATTTT GGG (shown in SEQ ID NO.7)
[0063] or
[0064] hRPL22L1-SiR(926)+:GATCCCCAAAGTGCTGATTATAGCTGTGTTCAAGAGACACAGCTATAATCAGCACTTTTTTTTA (shown in SEQ ID NO.8)
[0065] hRPL22L1-SiR(926)-:AGCTTAAAAAAAAGTGCTGATTATAGCTGTGTCTCTTGAACACAGCTATAATCAGCACTTT GGG (shown in SEQ ID NO.9)
[...
Embodiment 2
[0088] Example 2 Effect of Inhibiting the Expression of RPL22L1 on the Biological Behavior of UACC-1598 Cells
[0089] 1. Detection of RPL22L1 amplification in tumor cells
[0090] (1) Routine cell culture: the human ovarian cancer cell line UACC-1598 containing double microsomes was selected as the research object, and RPMI-1640 medium containing 10% fetal bovine serum was used in CO 2 Cultured in an incubator at 37°C.
[0091] (2) Detection of RPL22L1 amplification in UACC-1598 cells by DNA and mRNA levels:
[0092] For DNA level, normal human peripheral blood DNA and human embryonic kidney cell HEK293DNA were used as controls to detect the amplification of RPL22L1 in UACC-1598; for mRNA level, normal human ovarian tissue cDNA and human embryonic kidney cell HEK293cDNA were used as controls to detect RPL22L1 amplification Amplification in UACC-1598. The controls used in the above two levels are the samples that have been extracted in our laboratory earlier.
[0093] The ...
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