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38kDa antigen for Baylisascaris schroederi of giant pandas, preparation method for antigen and application of antigen

A technology of Ascaria schnitzel and giant panda, which is applied in the field of prevention and control of giant panda ascariasis, can solve the problem of lack of targeted protein antigens, etc., and achieve the effect of reducing the emergence of drug-resistant strains and reducing repeated infections

Inactive Publication Date: 2012-09-19
SICHUAN AGRI UNIV
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Problems solved by technology

However, to date, the number of protein antigens that can be used as specific vaccines for Ascaris schnitzel is still lacking; importantly, the precise immune mechanism involved remains to be determined, although previously published studies have shown that most stomach Intestinal parasitic nematode infection mainly induces a strong Th2 immune response in the host

Method used

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  • 38kDa antigen for Baylisascaris schroederi of giant pandas, preparation method for antigen and application of antigen
  • 38kDa antigen for Baylisascaris schroederi of giant pandas, preparation method for antigen and application of antigen
  • 38kDa antigen for Baylisascaris schroederi of giant pandas, preparation method for antigen and application of antigen

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Embodiment Construction

[0032] The following description will be made in conjunction with the examples and accompanying drawings. This example only takes experimental mice and rabbits as the implementation objects to illustrate the practicability of the Bsc38 antigen.

[0033] The gene of the antigen prepared in this example is the Bsc38 gene, and the nucleotide sequence of the gene is shown in Table 1: the nucleotide sequence of the mature peptide encoded by Bsc38, and the corresponding amino acid sequence is shown in Table 2.

[0034] 1. Extraction of RNA and amplification of Bsc38 gene

[0035] Extract the total RNA of Ascaris schnitzel, the concentration of the extracted RNA is about 1 μg / μL, and use the extracted 5 μL total RNA as a template, Oigo(dT) 18 For reverse transcription primers, the first cDNA synthesis was performed. Then use the synthesized cDNA as a template to amplify the Bsc38 gene; the amplification primers are designed as follows:

[0036] Upstream primer: 5'-TAAAGATGGCATTGGCC...

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Abstract

The invention discloses a 38kDa antigen for Baylisascaris schroederi of giant pandas, a preparation method for the antigen and application of the antigen. A gene of the antigen is a Bsc38 gene; a nucleotide sequence of the gene is shown in a table 1; the Bsc38 gene encodes a nucleotide sequence of mature peptide; and a corresponding amino acid sequence is shown in a table 2. A candidate antigen which is used for preparing a genetic engineering vaccine for preventing and controlling Baylisascaris schroederi disease of the giant pandas is identified and screened to solve the problems that Baylisascaris schroederi disease of the giant pandas is prepared and controlled mainly by medicines at present, medicine-resistant worm strains are caused by the frequent use of medicines, the pollution of food chains and environments is caused by chemical medicines, and the like. Compared with the prior art, the invention has the advantages that the antigen can reduce the medicine-resistant worm strains and the pollution of the medicines to the food chains and the environments in the medicine control process of Baylisascaris schroederi disease of the giant pandas, and can reduce the repeated infection of pandas in natural environments. The invention discloses a molecular immunologic mechanism after larvae of the Baylisascaris schroederi are infected with a host for the first time at home and abroad.

Description

technical field [0001] The invention belongs to the research field of prevention and treatment of giant panda ascariasis, and relates to an antigen of Ascaria schwae and the preparation and application of the antigen. Background technique [0002] As a national treasure of our country and a flagship species of wild animal protection, the giant panda is recognized as one of the most endangered species in the world. Currently, it is only distributed in five areas, namely Qinling Mountains, Mingshan Mountains, Daxiangling Mountains, Xiaoxiangling Mountains and Liangshan Mountains, east of the Qinghai-Tibet Plateau. Mountain system, the population is 2,500-3,000. Baylisascaris schroederi is the most common intestinal parasite in giant pandas, which is extremely harmful to wild and captive giant pandas. The adult worms of Ascaris schwali usually parasitize in the intestinal tract of giant pandas, but occasionally they can also appear in the mouth, larynx, trachea, stomach, pancr...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N15/12C12N15/70A61K39/00A61P33/10
Inventor 杨光友谢跃彭雪蓉陈世界余华严玉宝古小彬王淑贤
Owner SICHUAN AGRI UNIV
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