Panda oestrone peak time prediction method and application thereof

Abstract

The invention discloses a panda oestrone peak time prediction method and application thereof. The panda oestrone peak time prediction method comprises the steps of: when a female panda enters an oestrus season and the vulva color becomes red, collecting panda urine, and measuring the content of creatinine, oestrone and progesterone in the panda urine respectively; when the creatinine content is greater than 0.2 mg/mL, calculating the oestrone content after creatinine correction; when the oestrone content after creatinine correction is greater than 20 ng/mg Cr for the first time, indicating panda oestrus starting, recording a panda urine collection time point as oestrus starting time, extending backwards for 4 days from the starting time, performing E/P value calculation on panda urine of which the creatinine content is greater than 0.2 mg/mL and the progesterone content is greater than 2.5 ng/mL, and determining a target E/P value according to the E/P value; and determining appearancetime of a panda oestrone peak value according to the panda urine collection time point corresponding to the target E/P value. The panda oestrone peak time prediction method is high in prediction timeperiod accuracy degree, good in repeatability, simple to operate and small in workload.

Description

technical field [0001] The invention relates to the technical field of giant panda reproduction, in particular to a method for predicting the peak time of estrone in giant pandas and its application. Background technique [0002] The determination of the peak estrogen time is an important basis for the selection of timely mating time for giant pandas. However, there is still a lack of simple and reliable prediction methods, resulting in some individuals missing key samples and test results. choice poses difficulties. Giant pandas only estrus once a year, ovulate once a year, and have only one chance to reproduce a year. Timely prediction of the peak estrogen time will help timely detection and timely mating of giant pandas, avoiding wrong samples and testing. In the breeding of giant pandas in captivity, it is important to choose the right time to breed giant pandas after the peak of estrogen in the urine of female giant pandas. Therefore, finding the time of peak estrogen ...

AI Technical Summary

Problems solved by technology

For example, (Barbara S.Durran, 2006. Newtechnologies for the study of carnivore reproduction. It was reported that 3 female giant pandas showed cytological characteristics using vaginal cytology examination, of which 1 giant panda cytology examination was consistent with urine estrogen. The hormone peak relationship is as follows: on the 9th day before the estrogen peak, the basophils in the vaginal smear decreased by 50%, and the number of eosinophils became the dominant cell; on the 2nd day before the estrogen peak, the keratinocyte number became dominant cells, the other two have not been reported); however, this method has not been used by other giant panda breeding institutions, and it is not feasible when the number of giant pandas is large, and the training of giant pandas is difficult, and most individuals cannot collect cell samples; In addition, sampling from the giant panda’s vagina increases the stress response of giant pandas, which may affect the estrus and reproduction of giant pandas. Moreover, the identification and counting of cell types is subjective, and the results have large errors, which are difficult to quantify accurately. Time to estrogen peak results are inaccurate

[0004] The real-time detection method of estrogen requires intensive sampling and high-frequency estrogen level measurement, which increases the work intensity; for individual female giant pandas whose urine samples are difficult to collect, due to insufficient sampling, the urine sample corresponding to the estrogen peak may not be collected arrive

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