A method for predicting the peak time of estrone in giant pandas and its application

Abstract

The invention discloses a method for predicting the peak time of estrone in giant pandas and its application. The method includes collecting the urine of giant pandas when the female giant panda enters the estrous season, and measuring the urine of the giant pandas when the vulva turns red. The content of creatinine, estrone and progesterone in the medium; when the creatinine content is greater than 0.2mg / mL, calculate the creatinine-corrected estrone content; when the creatinine-corrected estrone content is greater than 20ng / mg Cr for the first time, it indicates the start of estrus in giant pandas , record the time point of this giant panda urine collection as the start of estrus time, and extend back 4 days from the enlightenment time, for the giant panda urine with creatinine content greater than 0.2 mg / mL and progesterone content greater than 2.5 ng / mL Calculate the E / P value, and determine the target E / P value according to the E / P value; determine the peak estrone occurrence time of the giant panda with the giant panda urine collection time point corresponding to the target E / P value; the prediction method predicts the time period High accuracy, good repeatability, simple operation and small workload.

Description

technical field [0001] The invention relates to the technical field of giant panda reproduction, in particular to a method for predicting the peak time of estrone in giant pandas and its application. Background technique [0002] The determination of the peak estrogen time is an important basis for the selection of timely mating time for giant pandas. However, there is still a lack of simple and reliable prediction methods, resulting in some individuals missing key samples and test results. choice poses difficulties. Giant pandas only estrus once a year, ovulate once a year, and have only one chance to reproduce a year. Timely prediction of the peak estrogen time will help timely detection and timely mating of giant pandas, avoiding wrong samples and testing. In the breeding of giant pandas in captivity, it is important to choose the right time to breed giant pandas after the peak of estrogen in the urine of female giant pandas. Therefore, finding the time of peak estrogen ...

AI Technical Summary

Problems solved by technology

For example, (Barbara S.Durran, 2006. Newtechnologies for the study of carnivore reproduction. It was reported that 3 female giant pandas showed cytological characteristics using vaginal cytology examination, of which 1 giant panda cytology examination was consistent with urine estrogen. The hormone peak relationship is as follows: on the 9th day before the estrogen peak, the basophils in the vaginal smear decreased by 50%, and the number of eosinophils became the dominant cell; on the 2nd day before the estrogen peak, the keratinocyte number became dominant cells, the other two have not been reported); however, this method has not been used by other giant panda breeding institutions, and it is not feasible when the number of giant pandas is large, and the training of giant pandas is difficult, and most individuals cannot collect cell samples; In addition, sampling from the giant panda’s vagina increases the stress response of giant pandas, which may affect the estrus and reproduction of giant pandas. Moreover, the identification and counting of cell types is subjective, and the results have large errors, which are difficult to quantify accurately. Time to estrogen peak results are inaccurate

[0004] The real-time detection method of estrogen requires intensive sampling and high-frequency estrogen level measurement, which increases the work intensity; for individual female giant pandas whose urine samples are difficult to collect, due to insufficient sampling, the urine sample corresponding to the estrogen peak may not be collected arrive

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