Method for measuring content of protein nitrogen in liquid

A determination method and technology for nitrogen content, applied in the field of analysis and determination of protein nitrogen, can solve the problems of inaccurate determination results of protein nitrogen content, insufficient digestion of nitrogen-containing compounds, affecting the digestion effect of nitrogen-containing compounds, etc. The effect of mild conditions and short treatment cycles
CN102539367AActive Publication Date: 2012-07-04CHINA TOBACCO GUANGDONG IND
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA TOBACCO GUANGDONG IND
Publication Date
2012-07-04

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Abstract

The invention provides a method for measuring content of protein nitrogen in liquid, which includes the following steps: separating protein nitrogen in liquid to obtain a to-be-measured sample containing nonprotein nitrogen; adding potassium persulfate into the to-be-measured sample that is being heated to obtain a mixed solution; ultraviolet catalyzing and high-temperature heating the mixed solution sequentially to obtain a digestion product; measuring the digestion product to obtain the content of nonprotein nitrogen in the liquid; and obtaining the content of protein nitrogen in the liquid as per the predeterminate total nitrogen content in the liquid and the content of nonprotein nitrogen obtained through measurement. Through adopting the method provided by the invention, the nitrogenous component in the liquid can be radically and completely digested, the digestion effect is better, the nitrogenous component as well as the reducing component in the liquid are digested, so that the liquid loses the reducing action, the interference in the digestion of the nitrogenous component is reduced, the uniformity of the digestion result is improved, the recovery rate in the content measurement of the protein nitrogen is improved, and the accuracy of the testing result is improved.
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Description

technical field

[0001] The invention relates to the technical field of analysis and determination of protein nitrogen, in particular to a method for determining protein nitrogen content in liquid. Background technique

[0002] Protein is an important substance that constitutes the structure of cells and organisms. It has the functions of catalysis, transportation, and information transmission. It can regulate the life activities of the body, enhance the body's resistance, regulate osmotic pressure, and supply heat energy. It plays a vital role in life activities. effect. Sufficient protein can maintain normal vital signs of living organisms, therefore, it is of great significance for the determination of protein content.

[0003] Each protein has its constant nitrogen content, but due to the complexity of protein composition and properties, the total nitrogen content is usually used to express the protein nitrogen content, so that the obtained protein nitrogen content also ...

Claims

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