Method for measuring content of protein nitrogen in liquid

A determination method and technology for nitrogen content, applied in the field of analysis and determination of protein nitrogen, can solve the problems of inaccurate determination results of protein nitrogen content, insufficient digestion of nitrogen-containing compounds, affecting the digestion effect of nitrogen-containing compounds, etc. The effect of mild conditions and short treatment cycles

Active Publication Date: 2012-07-04
CHINA TOBACCO GUANGDONG IND
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The above-mentioned high-pressure digestion has a good digestion effect on nitrogen-containing compounds in water bodies. However, for liquids with complex components, such as milk, this high-pressure digestion method has a good effect on certain nitrogen-containing substances such as plant alkaloids, amino acids, and certain substances. The digestion of some water-soluble protein and other nitrogen-containing components is not complete and thorough, and the obtained digestion products are not uniform, which brings large errors to the determination of nitrogen content; moreover, the liquid also contains reducing components, such as sugars, these reducing components It will react with oxidants, thereby affecting the digestion of nitrogen-containing components, making the digestion of nitrogen-containing compounds in the liquid not thorough enough, seriously affecting the digestion effect of nitrogen-containing compounds in the liquid, and making the determination of protein nitrogen content in the liquid inaccurate

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  • Method for measuring content of protein nitrogen in liquid
  • Method for measuring content of protein nitrogen in liquid
  • Method for measuring content of protein nitrogen in liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] 11 g of sodium nitrate was dissolved in 100 mL of distilled water to obtain a sodium nitrate stock solution with a mass concentration of 10.0%. Accurately pipette 1.0mL, 2.0mL, 4.0mL, 6.0mL, 8.0mL, 10.0mL of the standard stock solution, and use 0.5% acetic acid solution to dilute it to 100mL respectively to obtain a series of sodium nitrate standards solution to be tested. Take 2 mL of the standard solution to be tested respectively, pour it into the sample tube of the continuous flow analyzer and measure it in parallel three times respectively, and get the average value. In the continuous flow analyzer, the sample injection volume is 0.1mL / min, the injection time is 100s, and the flushing time is 120s. First at 90°C, add 2mL of potassium persulfate solution with a molar concentration of 0.3mol / L in the standard solution to be tested, the injection volume of potassium persulfate solution is 0.8mL / min, then the mixed solution obtained at 90 Under the acidic conditions ...

Embodiment 2～6

[0075] 2 mL of 5 kinds of milk samples were respectively selected, and the production dates of the milk samples were 20111010, 20111022, 20111031, 20111115, and 20111118 respectively. Add 50mL of acetic acid solution with a volume fraction of 0.5% to it, heat to boil for 15 minutes, filter quickly with nitrogen-free qualitative filter paper, wash the precipitate with 0.5% acetic acid solution by volume fraction, combine the obtained filtrates, and dilute to 200mL after cooling , to obtain the test solution of milk. Pipette 2 mL of the test solution of the milk, pour it into the sample tube of the continuous flow analyzer and measure it in parallel three times respectively, and get the average value. In the continuous flow analyzer, the sample injection volume is 0.1mL / min, the injection time is 100s, and the flushing time is 120s. First at 90 DEG C, in the solution to be tested of the milk, adding 2mL of potassium persulfate solution with a molar concentration of 0.3mol / L, th...

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Abstract

The invention provides a method for measuring content of protein nitrogen in liquid, which includes the following steps: separating protein nitrogen in liquid to obtain a to-be-measured sample containing nonprotein nitrogen; adding potassium persulfate into the to-be-measured sample that is being heated to obtain a mixed solution; ultraviolet catalyzing and high-temperature heating the mixed solution sequentially to obtain a digestion product; measuring the digestion product to obtain the content of nonprotein nitrogen in the liquid; and obtaining the content of protein nitrogen in the liquid as per the predeterminate total nitrogen content in the liquid and the content of nonprotein nitrogen obtained through measurement. Through adopting the method provided by the invention, the nitrogenous component in the liquid can be radically and completely digested, the digestion effect is better, the nitrogenous component as well as the reducing component in the liquid are digested, so that the liquid loses the reducing action, the interference in the digestion of the nitrogenous component is reduced, the uniformity of the digestion result is improved, the recovery rate in the content measurement of the protein nitrogen is improved, and the accuracy of the testing result is improved.

Description

technical field [0001] The invention relates to the technical field of analysis and determination of protein nitrogen, in particular to a method for determining protein nitrogen content in liquid. Background technique [0002] Protein is an important substance that constitutes the structure of cells and organisms. It has the functions of catalysis, transportation, and information transmission. It can regulate the life activities of the body, enhance the body's resistance, regulate osmotic pressure, and supply heat energy. It plays a vital role in life activities. effect. Sufficient protein can maintain normal vital signs of living organisms, therefore, it is of great significance for the determination of protein content. [0003] Each protein has its constant nitrogen content, but due to the complexity of protein composition and properties, the total nitrogen content is usually used to express the protein nitrogen content, so that the obtained protein nitrogen content also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/44
Inventor 孔浩辉程志颖张心颖马青
Owner CHINA TOBACCO GUANGDONG IND
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