Method for manufacturing raw materials of fodder with rich proteins from amino acid fermentation residual liquor

A protein feed and fermentation residue technology, which is applied in the field of amino acid fermentation residue fermentation to prepare protein-rich feed raw materials, can solve the problems of insufficient non-protein nitrogen nitrogen source, decline in the quality of amino acid products, waste, etc., and achieve high-efficiency protein feed The effect of raw material production method, feed cost reduction, and drying cost reduction

Inactive Publication Date: 2015-08-12
河南双成生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In amino acid (including glutamic acid, lysine, threonine, arginine, tryptophan, valine, etc.) NH4 + , controlling the appropriate non-protein nitrogen nitrogen source is a very delicate technology. Insufficient non-protein nitrogen nitrogen source will cause a part of the accumulation of α-ketoglutarate, which will eventually lead to low amino acid conversion rate; excessive non-protein nitrogen nitrogen source If there are too many amino acids, it will cause some amino acids to be converted into glutamine, or remain in the fermentation broth, resulting in a decline in the quality of amino acid products
In order to fully convert carbon sou

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The method for preparing a protein-rich feed material by fermenting arginine fermentation residue is as follows:

[0019] 1) Arginine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). The medium composition: Glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68-72 h.

[0020] 2) Extraction of arginine: cool the above fermented liquid to low temperature (4~8°C), add sodium hydroxide to adjust the pH to 10.76, crystallize crude arginine, and extract arginine with a belt filter. The rest is arginine fermentation residue.

[0021] 3) Preparation of ...

Embodiment 2

[0027] The method for preparing protein-rich feed material by fermentation of threonine fermentation residue is as follows:

[0028] 1) Threonine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into the liquid fermentation medium (filling volume: 60%). Medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12MPa for 20min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68~72h.

[0029] 2) Extraction of threonine: Cool the above fermented liquid to low temperature (4~8°C), adjust the pH to 6.16, crystallize crude threonine, extract threonine with a belt filter, and the rest is threonine Amino acid fermentation residue.

[0030] 3) Preparation of Candida tropicalis seed solution: Pic...

Embodiment 3

[0036] The method for preparing protein-rich feed material by fermentation of glutamic acid fermentation residue is as follows:

[0037] 1) Glutamic acid fermentation: Carry out glutamic acid fermentation according to the glutamic acid fermentation method introduced in "Condiment Fermentation Technology" (Edited by Song Andong, published by Chemical Industry Press in 2009).

[0038] 2) Extraction of glutamic acid: cool the above fermentation broth to low temperature (4~8°C), adjust the pH to 3.22, crystallize crude glutamic acid, extract glutamic acid with a belt filter, and the rest is gluten Amino acid fermentation residue.

[0039] 3) Preparation of Candida tropicalis seed solution: Pick an appropriate amount of strains from the activated Candida tropicalis slant numbered CICC1318 and put them into a culture bottle (250mL Erlenmeyer flask), add 40mL of wort medium into the culture bottle, 28 Cultivate with shaking at ℃ for 22 h, and cultivate at a rotational speed of 100 r...

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PUM

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Abstract

The invention discloses a method for manufacturing raw materials of fodder with rich proteins from amino acid fermentation residual liquor. The method includes inoculating and cultivating candida tropicalis or candida utilis and inoculating the candida tropicalis or the candida utilis into the amino acid fermentation residual liquor to obtain fermentation mash; adding honey into soybean meal and the like to manufacture solid fermentation raw materials; proportionally mixing the fermentation mash and the solid fermentation raw materials with one another, then carrying out solid-state fermentation on the fermentation mash and the solid fermentation raw materials, and drying and crushing the fermentation mash and the solid fermentation raw materials. The method has the advantages that a characteristic that residual non-protein nitrogen and residual sugar in the amino acid fermentation residual liquor can be converted into microbial cells by the candida tropicalis or the candida utilis is utilized, and the candida tropicalis or the candida utilis is inoculated into the amino acid fermentation residual liquor, so that the non-protein nitrogen and carbon sources which remain in the amino acid fermentation residual liquor during amino acid production can be converted into proteins of the microbial cells by the aid of the candida tropicalis or the candida utilis, and resources of the amino acid fermentation residual liquor can be sufficiently utilized; the non-protein nitrogen utilization rate further can be increased owing to solid-state fermentation, accordingly, the cost of the fodder can be reduced, and the method for efficiently manufacturing the raw materials of the fodder with the proteins is provided for developing the breeding industries.

Description

technical field [0001] The invention relates to the technical field of biological feed production, in particular to a method for preparing protein-rich feed raw materials by fermentation of amino acid fermentation residue. Background technique [0002] In amino acid (including glutamic acid, lysine, threonine, arginine, tryptophan, valine, etc.) NH4 + , controlling the appropriate non-protein nitrogen nitrogen source is a very delicate technology. Insufficient non-protein nitrogen nitrogen source will cause a part of the accumulation of α-ketoglutarate, which will eventually lead to low amino acid conversion rate; excessive non-protein nitrogen nitrogen source If there are too many amino acids, it will cause a part of the amino acid to be converted into glutamine, or remain in the fermentation broth, resulting in a decrease in the quality of the amino acid product. In order to fully convert carbon source substrates to form amino acids, non-protein nitrogen is usually added...

Claims

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Application Information

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IPC IPC(8): A23K1/00
Inventor 曹胜炎宋安东李伟苏丽娟杨森谢慧
Owner 河南双成生物科技有限公司
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