Method for manufacturing raw materials of fodder with rich proteins from amino acid fermentation residual liquor
A protein feed and fermentation residue technology, which is applied in the field of amino acid fermentation residue fermentation to prepare protein-rich feed raw materials, can solve the problems of insufficient non-protein nitrogen nitrogen source, decline in the quality of amino acid products, waste, etc., and achieve high-efficiency protein feed The effect of raw material production method, feed cost reduction, and drying cost reduction
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Embodiment 1
[0018] The method for preparing a protein-rich feed material by fermenting arginine fermentation residue is as follows:
[0019] 1) Arginine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). The medium composition: Glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68-72 h.
[0020] 2) Extraction of arginine: cool the above fermented liquid to low temperature (4~8°C), add sodium hydroxide to adjust the pH to 10.76, crystallize crude arginine, and extract arginine with a belt filter. The rest is arginine fermentation residue.
[0021] 3) Preparation of ...
Embodiment 2
[0027] The method for preparing protein-rich feed material by fermentation of threonine fermentation residue is as follows:
[0028] 1) Threonine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into the liquid fermentation medium (filling volume: 60%). Medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH7.0, sterilized at 0.12MPa for 20min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68~72h.
[0029] 2) Extraction of threonine: Cool the above fermented liquid to low temperature (4~8°C), adjust the pH to 6.16, crystallize crude threonine, extract threonine with a belt filter, and the rest is threonine Amino acid fermentation residue.
[0030] 3) Preparation of Candida tropicalis seed solution: Pic...
Embodiment 3
[0036] The method for preparing protein-rich feed material by fermentation of glutamic acid fermentation residue is as follows:
[0037] 1) Glutamic acid fermentation: Carry out glutamic acid fermentation according to the glutamic acid fermentation method introduced in "Condiment Fermentation Technology" (Edited by Song Andong, published by Chemical Industry Press in 2009).
[0038] 2) Extraction of glutamic acid: cool the above fermentation broth to low temperature (4~8°C), adjust the pH to 3.22, crystallize crude glutamic acid, extract glutamic acid with a belt filter, and the rest is gluten Amino acid fermentation residue.
[0039] 3) Preparation of Candida tropicalis seed solution: Pick an appropriate amount of strains from the activated Candida tropicalis slant numbered CICC1318 and put them into a culture bottle (250mL Erlenmeyer flask), add 40mL of wort medium into the culture bottle, 28 Cultivate with shaking at ℃ for 22 h, and cultivate at a rotational speed of 100 r...
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