Method for preparing fatty acid-rich protein feed raw material from amino acid fermentation residual liquor

A technology of fermentation residue and protein feed, applied in the direction of microorganism-based methods, biochemical equipment and methods, animal feed, etc., can solve problems such as waste, quality degradation of amino acid products, and environmental pollution, so as to reduce drying costs and improve utilization rate, the effect of protecting the environment

Inactive Publication Date: 2015-08-26
河南双成生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In amino acid (including glutamic acid, lysine, threonine, arginine, tryptophan, valine, etc.) NH4 + , controlling the appropriate non-protein nitrogen nitrogen source is a very delicate technology. Insufficient non-protein nitrogen nitrogen source will cause a part of the accumulation of α-ketoglutarate, which will eventually lead to low amino acid conversion rate; excessive non-protein nitrogen nitrogen source If there are too many amino acids, it will cause some amino acids to be converted into glutamine, or remain in the fermentation broth, resulting in a decline in the quality of amino acid products
In order to fully convert carbon sou

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for preparing a fatty acid-rich protein feed material by using arginine fermentation residue is as follows:

[0021] 1) Arginine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). The medium composition: Glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L, pH7.0, sterilized at 0.12MPa for 20min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68-72 h.

[0022] 2) Extraction of arginine: cool the above fermented liquid to low temperature (4~8°C), add sodium hydroxide to adjust the pH to 10.76, crystallize crude arginine, and extract arginine with a belt filter. The rest is arginine fermentation residue.

[0023] 3) Preparation...

Embodiment 2

[0029] The method for preparing a fatty acid-rich protein feed material by utilizing threonine fermentation residue is as follows:

[0030] 1) Threonine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). Medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L, pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68~72h.

[0031] 2) Extraction of threonine: Cool the above fermented liquid to low temperature (4~8°C), adjust the pH to 6.16, crystallize crude threonine, extract threonine with a belt filter, and the rest is threonine Amino acid fermentation residue.

[0032] 3) Preparation of Trichosporium viscoscensus see...

Embodiment 3

[0038] The method for preparing a fatty acid-rich protein feed material by using glutamic acid fermentation residue is as follows:

[0039] 1) Glutamic acid fermentation: Carry out glutamic acid fermentation according to the glutamic acid fermentation method introduced in "Condiment Fermentation Technology" (Edited by Song Andong, published by Chemical Industry Press in 2009).

[0040] 2) Extraction of glutamic acid: cool the above fermentation broth to low temperature (4~8°C), adjust the pH to 3.22, crystallize crude glutamic acid, extract glutamic acid with a belt filter, and the rest is gluten Amino acid fermentation residue.

[0041] 3) Preparation of Trichosporium viscoscensus seed liquid: Pick an appropriate amount of strains from the slant of activated Trichosporium viscoscens, put it into 25ml of wort seed medium in a 250ml Erlenmeyer flask, culture it with shaking at 28°C for 22 hours, and rotate at 150 r / Min, to obtain the first-level seed liquid; then through the ...

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Abstract

The invention discloses a method for preparing a fatty acid-rich protein feed raw material from amino acid fermentation residual liquor. The method comprises the following steps: performing inoculated culture on trichosporon mucoides first and then inoculating a trichosporon mucoides seed solution into the amino acid fermentation residual liquor to obtain fermented liquor; adding a proper amount of molasses into bean pulp and the like, and mixing uniformly to prepare a solid-state fermentation raw material; mixing the fermented liquor with the solid-state fermentation raw material at a certain ratio, and performing aerobic solid-state fermentation; drying a fermented material and grinding. Wastes are reasonably utilized, the utilization rate of the fermentation residual liquor in amino acid production is increased, the trichosporon mucoides capable of efficiently utilizing non-protein nitrogen is applied to the fermentation of the amino acid fermentation residual liquor, and residual non-protein nitrogen and sugar in the amino acid fermentation residual liquor are fully utilized for microbial conversion. Moreover, in combination with solid-state fermentation, the utilization rate of non-protein nitrogen is further increased, the drying cost is greatly reduced, the environment is protected, resources are saved, and the low-cost and fatty acid-rich protein feed raw material is obtained.

Description

technical field [0001] The invention relates to the technical field of biological feed production, in particular to a method for preparing feed raw materials rich in fatty acid protein by using amino acid fermentation residue. Background technique [0002] In amino acid (including glutamic acid, lysine, threonine, arginine, tryptophan, valine, etc.) NH4 + , controlling the appropriate non-protein nitrogen nitrogen source is a very delicate technology. Insufficient non-protein nitrogen nitrogen source will cause a part of the accumulation of α-ketoglutarate, which will eventually lead to low amino acid conversion rate; excessive non-protein nitrogen nitrogen source If there are too many amino acids, it will cause a part of the amino acid to be converted into glutamine, or remain in the fermentation broth, resulting in a decrease in the quality of the amino acid product. In order to fully convert carbon source substrates to form amino acids, non-protein nitrogen is usually a...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/16C12N1/20C12R1/645
Inventor 宋安东曹胜炎苏丽娟李伟杨森王风芹
Owner 河南双成生物科技有限公司
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