Method for preparing fatty acid-rich protein feed raw material from amino acid fermentation residual liquor
A technology of fermentation residue and protein feed, applied in the direction of microorganism-based methods, biochemical equipment and methods, animal feed, etc., can solve problems such as waste, quality degradation of amino acid products, and environmental pollution, so as to reduce drying costs and improve utilization rate, the effect of protecting the environment
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Embodiment 1
[0020] The method for preparing a fatty acid-rich protein feed material by using arginine fermentation residue is as follows:
[0021] 1) Arginine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). The medium composition: Glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L, pH7.0, sterilized at 0.12MPa for 20min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68-72 h.
[0022] 2) Extraction of arginine: cool the above fermented liquid to low temperature (4~8°C), add sodium hydroxide to adjust the pH to 10.76, crystallize crude arginine, and extract arginine with a belt filter. The rest is arginine fermentation residue.
[0023] 3) Preparation...
Embodiment 2
[0029] The method for preparing a fatty acid-rich protein feed material by utilizing threonine fermentation residue is as follows:
[0030] 1) Threonine liquid fermentation: use 200ml of sterile saline to make a suspension of Corynebacterium glutamicum on beef extract peptone, and inoculate it into a liquid fermentation medium (filling volume: 60%). Medium composition: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O 0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L, pH7.0, sterilized at 0.12 MPa for 20 min. Culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 68~72h.
[0031] 2) Extraction of threonine: Cool the above fermented liquid to low temperature (4~8°C), adjust the pH to 6.16, crystallize crude threonine, extract threonine with a belt filter, and the rest is threonine Amino acid fermentation residue.
[0032] 3) Preparation of Trichosporium viscoscensus see...
Embodiment 3
[0038] The method for preparing a fatty acid-rich protein feed material by using glutamic acid fermentation residue is as follows:
[0039] 1) Glutamic acid fermentation: Carry out glutamic acid fermentation according to the glutamic acid fermentation method introduced in "Condiment Fermentation Technology" (Edited by Song Andong, published by Chemical Industry Press in 2009).
[0040] 2) Extraction of glutamic acid: cool the above fermentation broth to low temperature (4~8°C), adjust the pH to 3.22, crystallize crude glutamic acid, extract glutamic acid with a belt filter, and the rest is gluten Amino acid fermentation residue.
[0041] 3) Preparation of Trichosporium viscoscensus seed liquid: Pick an appropriate amount of strains from the slant of activated Trichosporium viscoscens, put it into 25ml of wort seed medium in a 250ml Erlenmeyer flask, culture it with shaking at 28°C for 22 hours, and rotate at 150 r / Min, to obtain the first-level seed liquid; then through the ...
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