<15>N SIP (stable isotope probing) vibrio parahaemolyticus culture medium and cultivation method thereof

An isotope labeling and stable isotope technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high cost, many side reactions, complex process, etc., and achieve low cost and simple medium components. , The process is simple and reasonable

Inactive Publication Date: 2015-04-29
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former generally uses a medium containing heavy stable isotope essential amino acids to cultivate microorganisms, but no matter the method of microbial fermentation or organic chemical synthesis of stable isotope-labeled amino acids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • &lt;15&gt;N SIP (stable isotope probing) vibrio parahaemolyticus culture medium and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1:

[0029] The Vibrio parahaemolyticus strain is Vibrio parahaemolyticus ATCC 17802.

[0030] 1) 15 Preparation of N stable isotope labeling medium:

[0031] In this example 15 N stable isotope labeling medium is composed of NH 4 Cl, glucose, KH 2 PO 4 、K 2 HPO4, MgSO 4 , NaCl, distilled water; where NH 4 Cl content is 2g / L, glucose content is 10g / L, KH 2 PO 4 The content is 0.6g / L, K 2 HPO4 content is 0.9g / L, MgSO 4 The content of NaCl is 0.2g / L, the content of NaCl is 20g / L, and the content of distilled water is 1L; the pH value of the medium is 7.0-7.5; 15 NH in N stable isotope labeled medium 4 The nitrogen atom of Cl adopts 15 N was labeled (abundance, 98%).

[0032] 2) Continuous multiple transfer culture of Vibrio parahaemolyticus:

[0033] (1) Vibrio parahaemolyticus ATCC17802 was inoculated into 3% sodium chloride alkaline peptone water for the first enrichment, and cultured at 30°C for 18 hours on a shaking table. The formula of 3% so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a <15>N SIP (stable isotope probing) vibrio parahaemolyticus culture medium and a cultivation method thereof. A formula of the culture medium comprises components as follows: 1-5 g/L of a nitrogen source, 5-18 g/L of a carbon source, 0.5-2.0 g/L of KH2PO4, 0.6-2.0 g/L of K2HPO4, 0.1-1.0 g/L of MgSO4, 10-30 g/L of NaCl and 1L of distilled water, the pH is in a range from 7.0 to 7.5, and nitrogen atoms of the nitrogen source are labeled by adopting <15>N. The cultivation method of the <15>N SIP vibrio parahaemolyticus comprises steps as follows: 1), continuously and repeatedly cultivating the vibrio parahaemolyticus through transferring; 2), verifying the labeling efficiency of protein nitrogen atoms in labeled vibrio parahaemolyticus cells with LC-MS/MS (high-performance liquid chromatography-tandem mass spectrometry). The process is simple and reasonable, the culture medium has simple components and is low in cost, and the novel culture medium preparation method is provided for industrial preparation of the <15>N SIP vibrio parahaemolyticus culture medium.

Description

technical field [0001] The present invention relates to a 15 N stable isotope labeled Vibrio parahaemolyticus culture medium and its cultivation method. Background technique [0002] In the marine environment, most Vibrio species are the most common type of bacteria that maintain normal marine habitats, but some Vibrio species are harmful to humans and marine animals. In 1995, the sixth edition of the American Manual of Clinical Microbiology identified 12 types of pathogenic bacteria in Vibrio, the most important of which were Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus. FDA in the United States, NMKL in Europe, and the Ministry of Health in China have all formulated corresponding detection methods. Diseases caused by pathogenic Vibrio include gastroenteritis, wound infection, etc. For example, food poisoning caused by Vibrio parahaemolyticus is transmitted through improperly cooked seafood or salted products. The common ones are jellyfish, sea fish, s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12R1/63
CPCC12N1/20
Inventor 贾俊涛李正义魏晓棠姜英辉赵丽青王骏张健
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap