Method for rapidly detecting protein content in tobacco leaves

Abstract

The invention discloses a method for rapidly detecting the content of protein in tobacco leaves, which comprises the following steps: taking a certain amount of tobacco leaf powder to be detected in a homogenizer, adding 0.05 mol / L phosphate buffer solution containing SDS (sodium dodecyl sulfate) with the pH value of 6.8, fully grinding to obtain homogenate, carrying out ultrasonic oscillation on the homogenizer, then carrying out centrifugal separation, then carrying out centrifugal separation at the rotating speed of 4800r / min, taking the supernatant, filtering, and carrying out freeze drying to obtain the protein content in the tobacco leaves. According to the method, cell tissues are fully damaged in the homogenizing process, exudation of protein is facilitated, protein can be efficiently separated out from the screened protein precipitation solution, the influence of non-protein nitrogen is avoided, and the result is more accurate; compared with a Kendall method and a continuous flow analysis method, the method has the advantages that the steps are simplified, the digestion time is obviously shortened, and the total consumed time is less; the ultraviolet and visible spectrophotometer can realize simultaneous detection of a plurality of samples and has the characteristics of high efficiency and large flux, and other devices such as a centrifugal machine and a water bath kettle are standing devices in a laboratory.

Description

technical field [0001] The invention relates to the fields of tobacco leaf processing, alcoholization, cigarette production and the like, in particular to a method for rapidly detecting protein content in tobacco leaves. Background technique [0002] Tobacco is an important economic crop in my country and plays a very important role in the process of national economic and social development. Tobacco leaves contain many chemical substances, and the content of protein is the most abundant. After the protein is burned, with the generation of aza compounds, the smoke shows a bitter, pungent and irritating taste. Excessive protein has an adverse effect on the smoking taste of cigarettes, but if the protein content is too low, the smoke will appear flat, eating Taste and aroma also deteriorate. At the same time, protein can be decomposed into amino acids, and the content of amino acids has an obvious positive correlation with the aroma and fullness of smoke. Therefore, in the p...

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Problems solved by technology

The above methods all have their own advantages, but to a certain extent, there are problems such as cumbersome operation, long detection cycle, and unfriendly to the environment.

[0003] However, the existing technology has problems such as long detection period, unfriendly environment, and high requirements for detection instruments. Although the results of the Keldahl method and the Kjeldahl method are relatively accurate, the operation is time-consuming and complicated; the Lowry method has a narrow measurement range and a large amount of reagents. Long; at the same time, the Coomassie brilliant blue method is not suitable for mass detection

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