Detection method for on-site fast detection protein nitrogen

A detection method, protein nitrogen technology, which is applied in the field of food analysis, can solve the problems of high detection cost, high price, unsuitable detection, etc., and achieve the effect of simple, convenient and fast operation and low use cost

Inactive Publication Date: 2009-02-18
崔杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, this method uses specific large-scale equipment, which is expensive and the detection cost is very high, and it is not suitable for a large number of on-site detection

Method used

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  • Detection method for on-site fast detection protein nitrogen
  • Detection method for on-site fast detection protein nitrogen
  • Detection method for on-site fast detection protein nitrogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A detection method for rapidly detecting protein nitrogen on the spot, which is made by using the result obtained after the color reaction of protein standard substance with protein nitrogen content of 10-40% and iTAG reagent or Coomassie brilliant blue staining reagent or Folin-phenol reagent Standard color comparison card, take 0.2 g of milk powder of the sample to be tested, dilute to 10 ml with distilled water, take 2 g of the solution and add it to a 50 ml beaker, then add iTAG reagent or Coomassie brilliant blue staining reagent or Folin-phenol reagent 2 After mixing evenly, the color develops, and the content of protein nitrogen in the sample is read by comparing the card reader with the standard color card.

Embodiment 2

[0026] A detection method for on-site rapid detection of protein nitrogen, using a standard colorimetric card made from the result of the color reaction between a protein standard with a protein nitrogen content of 10-40% and iTAG reagent, and setting it on a handheld In the colorimeter, take 2 grams of milk as the sample to be tested, add 2 grams of distilled water to dilute, then take 2 grams of sample and add it to the sample hole of the hand-held colorimeter, then add 2 grams of iTAG reagent, mix well and develop color, and The content of protein nitrogen in the sample is read by comparison with the standard color card.

Embodiment 3

[0028] A colorimetric test paper, a standard colorimetric card made from the results obtained after the color reaction of a protein standard with a protein nitrogen content of 10-40% and iTAG reagent or Coomassie brilliant blue staining reagent or Folin-phenol reagent It consists of two parts, the test area coated with iTAG reagent or Coomassie brilliant blue staining reagent or Folin-phenol reagent.

[0029] The following samples are each taken in an appropriate amount (0.2 grams of solid samples are fixed to 10 milliliters and 2 grams are taken; 2 grams of liquid samples are taken), and the protein nitrogen content is measured by using this test paper, and then the nitrogen content is measured by the Kjeldahl method. See Table 1:

[0030] Table 1

[0031]

[0032] Then take the same sample and add 1% "melamine" according to the mass ratio, and use this test paper and the Kjeldahl method to detect the nitrogen content respectively. The results are shown...

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Abstract

Disclosed is a detection method of swiftly detecting protein nitrogen in situ, after the iTAG reagent which adopts the iTAG protein-labeled technique, or the coomassie brilliant blue method dyeing reagent, or the Folin- hydroxybenzene reagent swiftly reacts on the sample to be tested and presents color, the content of the protein nitrogen in the sample can be obtained through comparing the presented color with the standard colourimetric card. The cost of the detecting method is greatly lower than that of large-scale precision instrument, and the operation is simple and swift; although the precision is not enough, the content of protein nitrogen in milk from different batches can be swiftly obtained when food enterprises purchase mass raw material such as milk in situ, according to coloring result of the sample, whether the true protein (protein nitrogen) reaches the standard or not can be directly obtained; thereby being suitable for rough detection of mass sample in situ.

Description

technical field [0001] The invention relates to a food analysis method, in particular to a method for detecting protein nitrogen in food. Background technique [0002] At present, false proteins (such as "melamine" and its analogues) are illegally added to food and animal feed in the market, and traditional protein detection methods are helpless. The U.S. Food and Drug Administration (FDA) has adopted a pilot GC / MS method for the analysis of "melamine" in protein raw materials to screen for "melamine", "melamine diamide", "melamine monoamide" and "cyanuric acid" . [0003] "Melamine" (see picture for molecular formula) has a nitrogen content of 66.67%, is white and tasteless, and looks similar to protein powder. Adding 1% "melamine" can increase the nitrogen content of the test result by more than 4.1% in the nitrogen determination method. Driven by huge profits, lawbreakers add a large amount of non-protein nitrogen (ie: fake protein) to food and feed. [0004] [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/78
Inventor 崔杰崔蕴文
Owner 崔杰
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