Method for preparing mycoprotein by taking glycerin residue as raw material

A technology of bacterial protein and residue, which is applied in animal feed, animal feed, application, etc., can solve the problems of secondary pollution, increased cost investment, etc., and achieve the goal of reducing preparation cost, low cost, significant economic and social benefits Effect

Active Publication Date: 2013-12-25
惠州长龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned methods all deal with glycerin residues by adopting complex processes, which increases the input of various costs in the secondary recycling of glycerin residues, and produces secondary pollution at the same time.

Method used

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  • Method for preparing mycoprotein by taking glycerin residue as raw material
  • Method for preparing mycoprotein by taking glycerin residue as raw material
  • Method for preparing mycoprotein by taking glycerin residue as raw material

Examples

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Effect test

Embodiment 1

[0023] Embodiment 1 The method for preparing bacterial protein with glycerol residue as raw material

[0024] Take 80g of glycerin residue and dilute it with water to 400mL to prepare a glycerin residue dilution, add 1% peptone, 1% dipotassium hydrogen phosphate, 0.5% potassium dihydrogen phosphate, 0.05 % magnesium sulfate, 5% sodium chloride, after mixing evenly, adjust the pH value to 6.5 with hydrochloric acid. Distribute the culture medium into four 500 mL Erlenmeyer flasks with a liquid volume of 100 mL, and the remaining medium as seed culture medium into 50 mL Erlenmeyer flasks with a liquid volume of 10 mL, and autoclave at 121°C for 20 min to obtain the fermentation medium and seed medium.

[0025] Take 1% glucose, 0.5% peptone, 1% sodium chloride and 1.5% agar to make slant medium. Geotrichum candidum and Botrytis cinerea were selected and placed in the slant medium to cultivate the slant strains. The slant strains cultivated by Geotrichum candidum one-ring and...

Embodiment 2

[0029] Embodiment 2 is the method for raw material preparation bacterial protein with glycerol residue

[0030] Take 72 g of glycerin residue and dilute it to 400 mL with water to prepare a glycerin residue dilution, add 2% ammonium sulfate, 0.5% ammonium nitrate, and 1% dipotassium hydrogen phosphate to it, respectively. 0.5% potassium dihydrogen phosphate, 0.5% magnesium sulfate, 2.5% sodium chloride, after mixing evenly, adjust the pH value to 7 with hydrochloric acid. Distribute the medium into a 500 mL Erlenmeyer flask with a liquid volume of 100 mL, and the remaining medium as a seed medium into a 50 mL Erlenmeyer flask with a liquid volume of 10 mL, and autoclave at 121°C for 20 min. Fermentation medium and seed medium are obtained.

[0031] Take 2% glucose, 1% peptone, 1% sodium chloride and 1.5% agar to make slant medium. Candida tropicalis and Botrytis cinerea were selected and placed in the slant medium to cultivate the slant strains. Take one ring of Candida t...

Embodiment 3

[0035] Embodiment 3 is the method for raw material preparation bacterial protein with glycerol residue

[0036] Take 160g of glycerin residue and dilute it to 400ml with water to prepare a glycerin residue dilution, add yeast extract, 0.25% ammonium nitrate, 1% ammonium sulfate, 0.5% Sodium dihydrogen phosphate, 0.5% magnesium chloride, 1% sodium chloride, after mixing thoroughly, adjust the pH value to 7.5 with hydrochloric acid. Distribute the medium into a 500 mL Erlenmeyer flask with a liquid volume of 100 mL, and the remaining medium as a seed medium into a 50 mL Erlenmeyer flask with a liquid volume of 10 mL, and autoclave at 121°C for 20 min. Fermentation medium and seed medium are obtained.

[0037] Take 1.5% glucose, 0.75% peptone, 1% sodium chloride and 2% agar to make slant medium. Candida tropicalis and Geotrichum candidum were selected and placed in the slant medium to cultivate the slant strains. The slants cultured by two rings of Candida tropicalis and one...

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Abstract

The invention discloses a method for preparing mycoprotein by taking glycerin residue as a raw material, belonging to the field of biological feed. The method takes the glycerin residue as the main raw material, and concretely comprises the steps of diluting the glycerin residue by water to form glycerin residue solution with a certain concentration; adjusting the potential of hydrogen (pH) of the solution to be within the range from 6.5 to 7.5 by hydrochloric acid; then, adding non-protein nitrogen source, phosphate, magnesium salt and sodium chloride into the solution; carrying out liquid fermentation by microorganism; culturing for 72-96 hours at the temperature of 30-32 DEG C to obtain the mycoprotein. After the mycoprotein is added into the feed, the nutritive value of the basic feed can be enhanced, and the quality of animal products can be improved. The method takes the glycerin residue with low price as the main raw material, and can convert the glycerin residue waste produced in the glycerin distillation preparation into the mycoprotein by microbial fermentation, thus avoiding the environmental pollution caused by the glycerin residue on the one hand, and improving the economic benefit of the glycerin residue on the other hand.

Description

technical field [0001] The invention relates to a method for preparing bacterial protein by microbial fermentation and its application, in particular to a method for preparing bacterial protein by using glycerin residue as a raw material and its application in preparing animal feed, belonging to the field of biological feed. Background technique [0002] Glycerin residue is a mixture containing glycerin, polyglycerol, water, salt and other components produced after glycerin is processed by distillation process. Due to the complex components of the glycerin distillation residue, it is very difficult to remove impurities and decolorize it, resulting in the bottleneck of complicated and cumbersome subsequent treatment processes and high treatment costs. Therefore, glycerin residues have been diluted as waste for a long time and discharged or landfilled, causing serious environmental pollution. [0003] At present, the treatment and utilization of glycerin distillation residue ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/00
Inventor 陈列欢缪昕鑫张炜
Owner 惠州长龙生物技术有限公司
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