Preparation method for simple enzyme biosensor

A biosensor, a simple technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of long preparation cycle and unsuitable for large-scale production, and achieve good performance, accelerated electron transmission rate, and good reproducibility.

Active Publication Date: 2015-08-12
NANJING MEMBRANE IND TECH RES INST CO LTD
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Problems solved by technology

For example, the electrodeposition method is not suitable for large-scale production; the self-assembly method

Method used

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  • Preparation method for simple enzyme biosensor

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Effect test

Embodiment 1

[0023] A method for preparing an enzyme biosensor, comprising the following preparation steps:

[0024] (1) Configure 1mM·L -1 K 4 Fe(CN) 6 and FeCl 3 , synthesize Prussian blue by chemical method at equimolar concentration, the centrifugation rate is 3000r / min, and the centrifugation time is 30min;

[0025] (2) Mix the synthesized Prussian blue and carbon ink at a mass ratio of 1:1, and make it evenly mixed to prepare carbon ink / Prussian blue mixed ink;

[0026] (3) Enzyme biosensors were prepared on the support PVC by screen printing technology;

[0027] (4) preparing a glucose oxidase solution with a concentration of 500U / ml, and adding glutaraldehyde to the solution so that the mass percentage of glutaraldehyde in the glucose oxidase solution is 1%;

[0028] (5) Pipette the enzyme solution and apply it evenly on the working electrode 3 so that there is 20 μl of the enzyme solution per square centimeter of the working electrode 3, and place it in a refrigerator to dry ...

Embodiment 2

[0031] A method for preparing an enzyme biosensor, comprising the following preparation steps:

[0032] (1) Configure 100mM·L -1 K 4 Fe(CN) 6 and FeCl 3 , Prussian blue was synthesized by chemical method at equimolar concentration, the centrifugation rate was 10000r / min, and the centrifugation time was 3min;

[0033] (2) Mix the synthesized Prussian blue and carbon ink in a mass ratio of 1:99, and make it evenly mixed to prepare carbon ink / Prussian blue mixed ink;

[0034] (3) Enzyme biosensors were prepared on the support PET by screen printing technology;

[0035] (4) preparation concentration is the glucose oxidase solution of 5000U / ml, and adds glutaraldehyde in the solution, makes the mass percent concentration that glutaraldehyde accounts for in the glucose oxidase solution be 6%;

[0036] (5) Pipette the enzyme solution and apply it evenly on the working electrode 3 so that there is 200 μl of the enzyme solution per square centimeter of the working electrode 3, and...

Embodiment 3

[0039] A method for preparing an enzyme biosensor, comprising the following preparation steps:

[0040] (1) Configure 80mM·L -1 K 3 Fe(CN) 6 and (NH 4 ) 2 Fe(SO 4 ) 2 , Prussian blue was synthesized by chemical method at equimolar concentration, the centrifugation rate was 8000r / min, and the centrifugation time was 15min;

[0041] (2) Mix the synthesized Prussian blue and carbon ink in a mass ratio of 2:3, and make it evenly mixed to prepare carbon ink / Prussian blue mixed ink;

[0042] (3) Enzyme biosensors were prepared on the support alumina by screen printing technology;

[0043] (4) preparation concentration is the glucose oxidase solution of 2500U / ml, and adds glutaraldehyde in the solution, makes the mass percent concentration that glutaraldehyde accounts for in the glucose oxidase solution be 10%;

[0044] (5) Pipette the enzyme solution and apply it evenly on the working electrode 3 so that there is 120 μl of enzyme solution on the working electrode 3 per squar...

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Abstract

The invention discloses a preparation method for a simple enzyme biosensor. The method includes: mixing prussian blue ink with carbon ink in certain proportion, then preparing a substrate electrode by a screen printing technique, using glutaraldehyde as the cross-linking agent to fix the bio-oxidase corresponding to a detected substance so as to realize preparation of the enzyme sensor. The method is easy to operate and low in cost, can realize batch preparation, and the loaded enzyme is difficult to leak, thus ensuring high-activity of bio-enzyme. The enzyme biosensor has high sensitivity and stable electric signal response, can realize mass production, and is easy to achieve industrialization. The prepared enzyme biosensor is applicable to hydrogen peroxide or glucose detection in food analysis, clinical medicine, and bioengineering.

Description

technical field [0001] The invention relates to a simple preparation method of an enzyme biosensor, which is suitable for the detection of hydrogen peroxide or glucose in food analysis, clinical medicine and bioengineering. Background technique [0002] With the development of science and technology, people pay more and more attention to their own health and the detection of physiological substances. Therefore, the application of electrochemical biosensors has been unprecedentedly developed. Among electrochemical biosensors, enzyme sensors are the most representative, with the advantages of good selectivity, fast response and high sensitivity. Enzyme biosensors use enzymes as biological sensitive elements, and capture the measurable signals that are proportional to the concentration of the target substance generated by the reaction between the target substance and the sensitive element through various physical and chemical signal converters. Analytical instruments for the ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 金万勤储震宇姜丹锋
Owner NANJING MEMBRANE IND TECH RES INST CO LTD
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