55 results about "Chitin binding" patented technology

Chimeric genes encoding lectins exhibiting pesticidal activity (for example, insecticidal and / or fungicidal activity) are disclosed which can be used to transform cotton to yield cotton cells, plants, and seeds in which the chimeric genes are expressed. Such transformed cotton cells are pesticidal when ingested by cotton pests.

Chimeric genes encoding lectins exhibiting pesticidal activity (for example, insecticidal and / or fungicidal activity) are disclosed which can be used to transform cotton to yield cotton cells, plants, and seeds in which the chimeric genes are expressed. Such transformed cotton cells are pesticidal when ingested by cotton pests.

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

The invention discloses an insect-resistant fusion gene and an encoded protein as well as application thereof. The gene comprises a nucleotide sequence of encoding chitin binding protein and a nucleotide sequence of encoding Vip3 toxin in turn from 5' to 3'. The two nucleotide sequences are located in one opening reading frame. Compared with original chitin binding protein and by Vip3 protein, the artificial protein molecule formed by fusing one chitin binding protein and one Vip3 toxin designed by the invention has the advantages that the insecticidal spectrum is wide, various important pests (such as beet armyworm, armyworm and mealywing) in lepidoptera and hemiptera can be simultaneously prevented and controlled and the insecticidal efficiency reaches up to 50%-100%; the combined utilization of the proteins with different functions and other insect-resisting proteins (such as ICPs) is benefited, the insecticidal spectrum is further widened, and the generation of pest resistance is delayed.

The invention discloses a method for producing 1,5-pentanediamine through immobilized lysine decarboxylase. The method comprises the following steps of connecting a chitin binding domain (ChBD) gene to the N end of a lysine decarboxylase gene (CadA) to construct a fusion protein; fermenting the fusion protein to obtain a crude lysine decarboxylase enzyme solution; adding chitin into the crude enzyme solution to obtain immobilized lysine decarboxylase; finally, enabling the obtained immobilized lysine decarboxylase to participate in a decarboxylation reaction of L-lysine to prepare 1,5-1,5-pentanediamine. According to the method, the specific affinity effect of the substrate chitin and the fusion protein is skillfully utilized to adsorb the lysine decarboxylase, the cost of the immobilizedcarrier chitin is low, the immobilization efficiency is high, the activity stability of enzymes is high, and meanwhile, the procedure of protein purification can be omitted through specific affinity adsorption. The provided method achieves repeated production of 1,5-1,5-pentanediamine and can reduce the production cost, simplify the separation procedure of downstream products and enzymes and achieve remarkable economic benefits.

Compositions and methods are provided for creating and identifying mutant carbohydrate-binding proteins that reversibly bind to carbohydrate substrates under conditions where the native protein remains bound. Examples of modified chitin-binding domains are provided which can be eluted from chitin in the presence of a reducing agent or at a pH within the range of 5-10.

The invention relates to a chitin binding protein CBP58, and an encoding gene and an application thereof, and belongs to the technical field of gene engineering. The encoding gene of the chitin binding protein CBP58 is cloned from a strain pseudoalteromonas sp.DL-6 for the first time; and a high-activity expression product is obtained by construction of a prokaryotic expression vector and imidazole elution. The preparation process is simple; a simple and convenient method is provided for subsequent research on the chitin protein CBP58; the chitin protein CBP58 is low in production cost and easy to store, has a chitin binding function, is applied to purification and auxiliary identification of chitin, has a good inhibiting effect on aspergillus niger (Aspergillus niger) and cytospora mandshurica miura (Cytospora mandshurica miura), has a potential biological control function and has wide application value; a novel research direction is provided for research and development of anti-fungal medicines in the field of agriculture; and great industrial benefits and economic value are generated.

The present invention relates to chitin degradative systems, in particular to systems containing enzymes that bind to and depolymerize chitin. These systems have a number of applications. The present invention also describes enzymes with at least two catalytic domains in which the domains are separated by poly-amino acid linkers.

The present invention discloses genetically modified plants, such as potato plants. The plants are more resistant to a pathogen of interest following transformation of plant cells with a chimeric gene comprising a chitinase gene and β-1,3-glucanase gene. The invention also provides a method of enhancing the resistance of plants to pathogens by introducing a Brassica chitinase gene encoding two or more chitin-binding domains and β-1,3-glucanase gene and expressing the chitinase gene and β-1,3-glucanase gene.