The invention discloses a method for producing 1,5-pentanediamine through immobilized
lysine decarboxylase. The method comprises the following steps of connecting a
chitin binding domain (ChBD)
gene to the N end of a
lysine decarboxylase
gene (CadA) to construct a
fusion protein; fermenting the
fusion protein to obtain a crude
lysine decarboxylase
enzyme solution; adding
chitin into the crude
enzyme solution to obtain immobilized
lysine decarboxylase; finally, enabling the obtained immobilized
lysine decarboxylase to participate in a
decarboxylation reaction of L-lysine to prepare 1,5-1,5-pentanediamine. According to the method, the specific affinity effect of the substrate
chitin and the
fusion protein is skillfully utilized to adsorb the
lysine decarboxylase, the cost of the immobilizedcarrier chitin is low, the immobilization efficiency is high, the activity stability of enzymes is high, and meanwhile, the procedure of
protein purification can be omitted through specific affinity adsorption. The provided method achieves repeated production of 1,5-1,5-pentanediamine and can reduce the production cost, simplify the
separation procedure of downstream products and enzymes and achieve remarkable
economic benefits.