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A method for preparing animal feed by using distiller's grains and puffed corn flour to cultivate Bacillus licheniformis

A technology of Bacillus licheniformis and puffed corn flour, which is applied in animal feed, animal feed, microbial-based methods, etc., can solve the problems of insufficient animal nutrition supply, limited protein content improvement, and affecting animal growth, etc.

Active Publication Date: 2021-04-20
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, distiller's grains have been used for animal feed after solid-state fermentation. However, the protein content in the fermented material is limited. When fed as animal feed alone, it will still lead to insufficient animal nutrition supply and affect the technical problems of animal growth. Therefore, it is often necessary to mix and feed other high-protein raw materials to meet the nutritional needs of animals for growth. How to further increase the nutrient content in fermented distiller's grains so that they can meet the nutrient requirements necessary for animal growth when fed alone important meaning

Method used

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  • A method for preparing animal feed by using distiller's grains and puffed corn flour to cultivate Bacillus licheniformis
  • A method for preparing animal feed by using distiller's grains and puffed corn flour to cultivate Bacillus licheniformis
  • A method for preparing animal feed by using distiller's grains and puffed corn flour to cultivate Bacillus licheniformis

Examples

Experimental program
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Effect test

Embodiment 1

[0036] (1) Preparation of Bacillus licheniformis seed liquid:

[0037] The lyophilized powder of Bacillus licheniformis was inoculated into the slant medium of potato medium for activation culture, and then transferred to liquid medium for cultivation. The number of viable bacteria detected was not less than 1×10 9 After cfu / mL, it was used as Bacillus licheniformis seed solution;

[0038]Liquid medium: 200g of potatoes, cut into pieces, add 1000ml of water, boil for 30min, filter through gauze, add 20g of sucrose, add water to 1000ml, adjust the pH value to 7.0, and autoclave;

[0039] (2) Preparation of fermented raw materials: mix distiller's grains and puffed corn flour evenly with a mass ratio of 8:2, and then adjust the pH to 7.0 with calcium oxide;

[0040] (3) Fermentation culture: Add the Bacillus licheniformis seed liquid prepared in step (1) into the fermentation raw materials, mix and spread it in the fermentation tank, the thickness of the laying is 4-10cm, and c...

Embodiment 2

[0043] The steps are the same as in the embodiment (1), except that the puffed corn flour in the fermentation raw material is replaced with corn flour to prepare the feed.

Embodiment 3

[0045] The steps are the same as in Example (1), except that 10 g of distiller's grains and 2 g of puffed corn flour are added to the liquid medium to prepare the feed.

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Abstract

The invention discloses a method and application for preparing feed by cultivating Bacillus licheniformis by using distiller's grains and expanded corn flour. Distiller's grains, a solid by-product of fermented liquor, are used as main raw materials, added with expanded corn flour, and activated Bacillus licheniformis is used as seeds Liquid, solid-state fermentation, distiller's grains through microbial fermentation can increase its protein content, reduce cellulose content, degrade anti-nutritional substances, thereby improving the nutritional value and palatability of distiller's grains, the final fermentation product of Bacillus licheniformis spores can reach 1×10 9 cfu / mL (wet weight), Bacillus is distributed in the feed in the form of endospores. After the spores enter the stomach of animals, they secrete a large amount of digestive enzymes, which enhance the ability of animals to digest complex compounds such as vegetable protein, fat, and starch, and stimulate intestinal peristalsis. , Strengthen the health of intestinal microorganisms and promote the growth and development of immune organs, improve the antibody level of the body, promote the growth of animals, reduce breeding costs and increase benefits.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for cultivating bacillus licheniformis by using distiller's grains and puffed corn flour to prepare animal feed. Background technique [0002] Distiller's grains are the main by-product produced in the brewing process, and the price is low. my country is a big producer of liquor, with an annual output of more than 27 million tons of various distiller's grains, and the supply is very sufficient. Due to the high water content, high acidity and strong viscosity of distiller's grains, it is easy to spoil and deteriorate, it is not easy to store, and the BOD value is extremely high. If it is directly discharged into the river without treatment, it will not only waste precious resources, but also cause serious environmental pollution. The main components of distiller's grains are protein, cellulose, fat, vitamins and various trace elements, so liquor production enterpris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/38A23K10/30A23K20/24A23K20/26A23K20/20C12N1/20C12R1/10
CPCA23K10/12A23K10/30A23K10/38A23K20/24A23K20/26A23K20/30C12N1/20Y02P60/87
Inventor 彭楠常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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