Method for regulating and controlling grain size of microbial-induced deposited calcite

A grain size, induced deposition technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, complex process, single size of calcium carbonate particles, etc., to achieve stable mineral properties, slow down The effect of the greenhouse effect

Inactive Publication Date: 2020-04-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of depositing calcium carbonate is mainly chemical method, including carbonization method, calcium chloride method and caustic alkali method, etc. The particle size of calcium carbonate synthesized by these methods is relatively single, and the process is complicated and the cost is high

Method used

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  • Method for regulating and controlling grain size of microbial-induced deposited calcite
  • Method for regulating and controlling grain size of microbial-induced deposited calcite
  • Method for regulating and controlling grain size of microbial-induced deposited calcite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Inoculate the photosynthetic bacteria into the sterilized medium solution, and each liter of medium contains NH 4 Cl 1.0 g, CH 3 COONa 3.0g, MgCl 2 0.1g, CaCl 2 0.1g, KH 2 PO 4 0.5g, K 2 HPO 4 0.4g, 0.1g of yeast extract, and control the pH to 7, shake culture at 30°C for 24 hours to obtain a bacterial liquid containing photosynthetic bacteria, and the concentration of the bacterial cells contained in the bacterial liquid is 10 6 individual / mL.

[0033] (2) Add calcium source and urea to the above-mentioned microbial bacterial liquid respectively, place in a constant temperature and humidity environment, the temperature is 30°C, and the standing time is 120 hours, wherein the mass ratio of calcium source, urea, and bacterial liquid is 1:0.6 :96.8;

[0034](3) After the precipitation occurs, the sediment is fully washed, filtered, dried, dried to a constant weight, and the grain size analysis is performed to obtain the characteristics of the photosyntheti...

Embodiment 2

[0046] (1) Inoculate the photosynthetic bacteria into the sterilized medium solution, and each liter of medium contains NH 4 Cl 1.5g, CH 3 COONa 4.0g, MgCl 2 0.2g, CaCl 2 0.2g, KH 2 PO 4 0.6g, K 2 HPO 4 0.5g, 0.2g of yeast extract, and control the pH to 8, shake culture at 35°C for 24h to obtain a bacterial liquid containing photosynthetic bacteria, and the concentration of the bacterial cells contained in the bacterial liquid is 10 7 individual / mL.

[0047] (2) Add calcium source and urea to the above-mentioned microbial bacterial liquid respectively, place in a constant temperature and humidity environment, the temperature is 35°C, and the standing time is 150 hours; wherein the mass ratio of calcium source, urea, and bacterial liquid is 2:1.2 :98.4

[0048] (3) After the precipitation occurs, the sediment is fully washed, filtered, dried, dried to a constant weight, and the grain size analysis is carried out to obtain the characteristics of the photosynthetic ba...

Embodiment 3

[0050] (1) Inoculate the photosynthetic bacteria into the sterilized medium solution, and each liter of medium contains NH 4 Cl 1.3g, CH 3 COONa 3.5g, MgCl 2 0.15g, CaCl 2 0.15g, KH 2 PO 4 0.55g, K 2 HPO 4 0.45g, 0.15g of yeast extract, and control the pH to 7.5, shake culture at 32°C for 24 hours to obtain a bacterial liquid containing photosynthetic bacteria, and the concentration of the bacterial cells contained in the bacterial liquid is 10 6 individual / mL.

[0051] (2) Add calcium source and urea to the above-mentioned microbial bacterial liquid respectively, place in a constant temperature and humidity environment, the temperature is 32°C, and the standing time is 130 hours; wherein the mass ratio of calcium source, urea, and bacterial liquid is 1.5:0.8 :97.7

[0052] (3) After the precipitation occurs, the sediment is fully washed, filtered, dried, dried to a constant weight, and the grain size analysis is carried out, measured and calculated according to th...

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Abstract

The invention provides a method for regulating and controlling the grain size of microbial-induced deposited calcite. The method includes the following steps: 1) inoculating endospores of Bacillus mucilaginosus or endospores of photosynthetic bacteria or endospores of Bacillus pasteurii to corresponding culture mediums separately so as to obtain a concentrated bacterial solution of Bacillus mucilaginosus or a concentrated bacterial solution of photosynthetic bacteria or a concentrated bacterial solution of Bacillus pasteurii separately; 2) adding a calcium source and urea to the concentrated bacterial solution of Bacillus mucilaginosus or the concentrated bacterial solution of photosynthetic bacteria or the concentrated bacterial solution of Bacillus pasteurii obtained in the step 1), andperforming still standing; and 3) after production of precipitates, washing the precipitates, performing filtration and drying, performing drying until constant weight, and then analyzing the grain size so as to prepare the calcite with a grain size of 20-60 nm. The microbial method has the characteristics of high efficiency and environmental protection, the grain size of the calcite can be adjusted according to needs, the formed calcite mineral has stable properties, and effective capture and utilization of carbon dioxide can be achieved in the process, so that the greenhouse effect is sloweddown.

Description

technical field [0001] The invention relates to a method for regulating the performance of a base material, in particular to a method for regulating the crystal grain size of calcite induced by microorganisms. Background technique [0002] Biomineralization refers to the process of generating inorganic minerals by organisms through the regulation of biological macromolecules. The biggest difference from general mineralization lies in the participation of biological cells, metabolites or organic substrates. As the most abundant and widely distributed life form on the earth, microorganisms have the ability to induce the deposition of carbonate, phosphate, sulfate and other minerals, so microorganisms play an important role in the process of biomineralization. The most important difference between the process of biomineralization and ordinary mineralization is that biominerals with special multi-level structures and assembly methods are generated through the interface interacti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12R1/07
CPCC12P3/00C12P39/00
Inventor 钱春香陈燕强张旋芮雅峰张耀明王凯
Owner SOUTHEAST UNIV
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