Microbial soil improvement agent for petroleum polluted soil

A soil conditioner and oil pollution technology, applied in the field of soil environment, can solve problems such as high cost, ineffective effect, and slow processing

Inactive Publication Date: 2016-11-16
麟州(巨野)孵化器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a microbial soil conditioner for petroleum-contaminated soil in view of the problems that the existing oil-contaminated soil remediation process is very slow, the effect is not obvious and the cost is high, and various types of microorganisms in the conditioner cooperate with each other Cooperate with it and work together with its carrier raw materials, using this modifier to treat different types of crude oil contaminated soil in Shengli Oilfield, the total removal rate of petroleum hydrocarbons in the contaminated soil reached 77.68% to 89.76%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The microbial soil improvement agent used for oil-contaminated soil is composed of the following raw materials with the weight of g: Mycelia of Structur perforatus

[0047] 7g, Umbrella burr 4g, Acinetobacter calcoacetate 12g, Sphingomonas 17g, Pyrene-degrading bacteria 10g, Glomus 0.5g, Cephalosporium pink 0.9g, Aureobasidium pullulans 0.4g, Nitrile Hydrolase 0.08g, long-chain alkane degrading enzyme 2g, integral membrane diferric alkane hydroxylase 1.5g, phenylalanine deaminase 0.15g, arginine dihydrolase 0.05g, Rhodococcus erythropolis 0.21g, aeruginosa Pseudomonas 0.18g, sodium oleate 1g, thiamine hydrochloride 0.08g, calcium pantothenate 0.11g, oyster shell powder 7g, vermiculite powder 4g, broadleaf paspalum powder 3g, Kentucky bluegrass powder 2g, pistachio powder 1.2 g, alfalfa powder 2.8g, pig manure grain 5g, sheep manure grain 13g, γ-polyglutamic acid 0.5g, malic acid 0.7g, citric acid 0.5g and Nostoc algae powder 3g.

[0048] Both the oyster shell powder an...

Embodiment 2

[0054] The microbial soil improvement agent used for oil-contaminated soil is composed of the following raw materials by weight g: Column mushroom

[0055] 15g of silk, 9g of velvety umbrella, 23g of Acinetobacter calcoacetate, 25g of Sphingomonas, 16g of Pyrene-degrading bacteria, 1.2g of Glomus spp., 1.8g of Cephalosporium pink, 1.0g of Aureobasidium pullulans, Nitrilase 0.15g, long-chain alkane degrading enzyme 5g, integral membrane diferric alkane hydroxylase 4.5g, phenylalanine deaminase 0.27g, arginine dihydrolase 0.09g, Rhodococcus erythropolis 0.35g, Pseudomonas aeruginosa 0.25g, sodium oleate 5g, thiamine hydrochloride 0.14g, calcium pantothenate 0.18g, oyster shell powder 13g, vermiculite powder 8g, paspalum powder 6g, Kentucky bluegrass powder 7g, pistachio powder 2.1g, 3.5g of alfalfa powder, 15g of pig manure grains, 20g of sheep manure grains, 1.8g of γ-polyglutamic acid, 1.3g of malic acid, 1.5g of citric acid and 9g of nostoc powder.

[0056] Both the oyster she...

Embodiment 3

[0062] The microbial soil improvement agent used for oil-contaminated soil is composed of the following raw materials by weight g: Column mushroom

[0063] 13g of silk, 7g of velvety umbrella, 20g of Acinetobacter calcoacetate, 21g of Sphingomonas, 13g of Pyrene-degrading bacteria, 0.9g of Glomus japonicus, 1.4g of Cephalosporium pink, 0.7g of Aureobasidium pullulans, Nitrilase 0.11g, long-chain alkane degrading enzyme 3.5g, integral membrane diferric alkane hydroxylase 3g, phenylalanine deaminase 0.21g, arginine dihydrolase 0.07g, Rhodococcus erythropolis 0.29g, Pseudomonas aeruginosa 0.21g, sodium oleate 3.5g, thiamine hydrochloride 0.11g, calcium pantothenate 0.13g, oyster shell powder 9g, vermiculite powder 6g, broadleaf paspalum powder 4.5g, Kentucky bluegrass powder 5.5g, five Mango powder 1.8g, alfalfa powder 3.1g, pig manure grains 9g, sheep manure grains 17g, γ-polyglutamic acid 1.0g, malic acid 0.9g, citric acid 1.1g and nostoc powder 7.5g.

[0064] Both the oyster ...

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Abstract

The present invention belongs to the technical field of soil environments, and specifically relates to a microbial soil improvement agent for petroleum polluted soil. The microbial soil improvement agent comprises the following raw materials: Paxillus involutus Hypha, Hebeloma mesophaeusm, Acinetobacter calcoaceticus, Sphingomonas paucimobilis, pyrene degrading bacteria, Glomus etunicatum, Cephalosporium roseum, Aureobasidium pullulans, nitrilase, long-chain alkane degradation enzyme, integration membrane di iron alkane hydroxylase, phenylalanine deaminase, arginine dihydrolase, Rhodococcus erythropolis, pseudomonas aeruginosa, sodium oleate, thiamine hydrochloride, calcium pantothenate, oyster shell powder, vermiculite powder, paspalum wettsteinii hackel powder, kentucky eupatorium fortunei powder, miscanthus floridulus powder, medicago sativa powder, pig manure granules, goat manure granules, gamma-polyglutamic acid, malic acid, citric acid, and Nostocales powder. According to the present invention, various types of the microorganisms in the improvement agent synergistically coordinate, and co-act with the carrier raw materials; and with the application of the improvement agent to treat different types of crude oil polluted soil in the Shengli oil field, the total removal rate of the petroleum hydrocarbon in the polluted soil achieves 77.68-89.76%.

Description

technical field [0001] The invention belongs to the technical field of soil environment, and in particular relates to a microbial soil improver for oil-polluted soil. Background technique [0002] With the exploration and development of petroleum and the continuous development of regional economy, a large amount of solid waste such as crude oil, oil sand, cuttings, mud, etc. have been produced everywhere in the oil field. These pollutants can cause changes in the physical and chemical properties of the soil and deteriorate the soil structure. , Reduce permeability, block soil pores, change the composition and structure of soil organic matter, cause changes in the structure of soil microbial communities, and then affect the ecological environment of the soil. [0003] Since the main components of petroleum are alkane, benzene, toluene, xylene and other complex aromatic hydrocarbons, these substances are highly toxic, some have carcinogenic and mutagenic effects, and are diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40
CPCC09K17/40
Inventor 荣琨郑焕强邹美玲罗杰张晨曦李学平
Owner 麟州(巨野)孵化器有限公司
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