Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof

A technology of LH-N13 and Rhodococcus erythrococcus, which is applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, can solve the problems of less research on denitrification and denitrification, and no literature reports on microbial strains, so as to solve the problem of total nitrogen hard-to-reach effect

Active Publication Date: 2015-05-13
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on denitrification and denitrification under high-salt conditions, and there are no literature rep

Method used

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  • Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof
  • Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, a kind of Rhodococcus red city ( Rhodococcus erythropolis ) LH-N13, its deposit number is CGMCC No. 6974.

Embodiment 2

[0017] Embodiment 2, a kind of microbial inoculum of Rhodococcus erythropolis, this microbial inoculum comprises the Rhodococcus erythrococcus described in embodiment 1 ( Rhodococcus erythropolis ) LH-N13. Prepared by conventional methods.

[0018] Rhodococcus red city described in embodiment 1 ( Rhodococcus erythropolis ) LH-N13 or a microbial agent of Rhodococcus red city described in Example 2 can be used to treat wastewater containing ammonia nitrogen. Especially high-salt wastewater containing ammonia nitrogen. When treating wastewater containing ammonia nitrogen, denitrification is carried out in the form of aerobic denitrification. The salt concentration of the high-salt wastewater is calculated as NaCl in the range of 1% to 10%, preferably ≤5%.

Embodiment 3

[0019] Embodiment 3, Rhodococcus red city ( Rhodococcus erythropolis ) Screening and identification method of LH-N13:

[0020] (1) Take 1g of a saltworks sludge sample and put it in 250ml of nitrogen-containing medium ((NH 4 ) 2 SO 4 0.5g; Glucose 1g; Na 2 CO 3 0.25g; FeCl 2 0.05g; MgSO 4 0.02g; MnSO 4 0.01g; NaCl (≤10%); pH: 7.0~8.0, distilled water 1L) Erlenmeyer flask, 150rpm, 30 ℃ shaker culture, 2d as a cycle, transfer to fresh medium at the end of each cycle, culture three cycle, domestication and enrichment of bacteria;

[0021] (2) After the cultivation, the plate dilution method was used for the primary screening of the strains. First, the enriched bacteria were diluted in equal proportions, and diluted to 10 -1 、10 -2 、10 -3 ,... 10 -7 bacterial suspension. then take 10 -5 、10 -6 、10 -7 Each 0.1mL of bacterial suspension was evenly spread on the pre-prepared high-salt denitrification solid medium (sodium succinate 0.5g, KNO 3 0.3g, NaNO 2 0.2...

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Abstract

The invention relates to a salt-tolerant aerobic denitrification bacterium rhodococcus erythropolis LH-N13 with a collection number of CGMCC No. 6974. The invention also discloses a microbial agent of rhodococcus erythropolis and an application of the microbial agent in high-salinity nitrogen-containing wastewater. The strain is screened from salt field sludge and has strong tolerance against high salinity; under the condition of the salinity (based on NaCl) less than or equal to 15%, nitrate nitrogen and nitrite nitrogen in the sewage can be removed, the microbial agent prepared from the strain can be directly used for denitrifying high-salinity wastewater, can be used for solving the difficult problem that the biological nitrogen removal total nitrogen is difficult to meet the standard under a high-salinity environment and has good application prospects. The salt-tolerant denitrification bacterium rhodococcus erythropolis LH-N13 or the microbial agent prepared from rhodococcus erythropolis LH-N13 can be used for the biological treatment of the high-salinity nitrogen-containing wastewater produced in food processing, bio-brewing, pesticides and leather.

Description

technical field [0001] The invention relates to a salt-tolerant aerobic denitrifying Rhodococcus erythrococcus ( Rhodococcus erythropolis ) LH-N13, its microbial agent and its application in the treatment of high-salt nitrogen-containing wastewater. Background technique [0002] In industries such as seafood processing, salted products processing, chemical industry, petroleum and aquaculture, most of the discharged nitrogen-containing wastewater has the characteristics of high salt. For example, metal refining wastewater contains a large amount of nitrate nitrogen, and seafood processing wastewater generally contains a large amount of protein and ammonia nitrogen. High-salt wastewater generally refers to wastewater with a total salt content of at least 1%. In many industrial practices such as seawater substitution, the salt content is sometimes as high as 15-30%. At present, the production methods and output of high-salt wastewater are increasing. The amount of wastewater ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 田凤蓉王开春李学字张彬彬杨志林董自斌何一群刘娟王克云
Owner BLUESTAR LEHIGH ENG INST CO LTD
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