Astragalus growth-promoting endophytic bacteria and its growth-promoting method and application

A technology of endophytic bacteria and astragalus, applied in the direction of microorganism-based methods, biochemical equipment and methods, plant growth regulators, etc.

Active Publication Date: 2021-09-28
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the current problem of promoting growth and improving quality of Astragalus membranaceus, the present invention provides endophytic bacteria for promoting growth of Astragalus membranaceus and its growth-promoting method and application

Method used

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  • Astragalus growth-promoting endophytic bacteria and its growth-promoting method and application
  • Astragalus growth-promoting endophytic bacteria and its growth-promoting method and application
  • Astragalus growth-promoting endophytic bacteria and its growth-promoting method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Determination of the volatile components of the main green leaves of Astragalus membranaceus

[0017] The 2, 5, and 7-year-old fresh Astragalus membranaceus from Hunyuan, Shanxi Province were used as materials, and aseptically separated into periderm, secondary phloem, and secondary xylem. After grinding with liquid nitrogen, 0.2 g samples were taken, and headspace GC-MS was used to analyze its Volatile Spectrum. With n-hexanal, E - 2 -Hexenal and n-hexanol were used as reference substances, and the relevant substances in the samples were identified according to their representative ion fragments and retention time. According to the peak areas of the three in the sample, calculate their relative content. GraphPad Prism (5.01) was used for data processing and significant difference analysis, and the threshold was set at P<0.05.

[0018] For a representative total ion current chromatogram see figure 1 . It can be seen from the figure that different anato...

Embodiment 2

[0023] Example 2: Determination of endophytic strains containing ACC deaminase and other life-promoting activities in Radix Astragali

[0024] The 5-year-old fresh roots of Astragalus membranaceus in Hunyuan, Shanxi Province were used as materials, and the endophytic bacteria of Astragalus membranaceus were isolated by tissue block separation method. The specific operation is as follows: wash the harvested fresh Astragalus membranaceus several times with tap water, wash away the soil remaining on the root surface, and then rinse it with sterile water containing 10% Tween-20; Cut into 1 cm long sections, soak in 75% alcohol for 1 min, rinse with sterile water for 3 times, soak in 5% NaClO for 10 min, rinse with sterile water for 3 times, and then rinse with 75% alcohol for 40 seconds. Rinse with sterile water for 5 times, absorb excess water with sterile filter paper, put the processed material in a sterile mortar, add 2 mL of normal saline to mash, and grind.

[0025] Take 10...

Embodiment 3

[0050] Example 3: Determination of n-hexanal growth-promoting bacterial strain and its concentration range

[0051] With 50% ethanol as solvent, prepare 2, 10, 50, 250, 1250, 6250 mM E-2 - The hexenal stock solution is added to the LB liquid medium according to the addition amount of 1‰ volume. 1‰ of the inoculum, the above strains were inoculated with different concentrations of E- 2 - LB medium of hexenal, cultured on a shaker at 28°C until logarithmic growth phase, and measured OD600. Those who added the same volume of sterile water and 50% ethanol were used as blank control and solvent control respectively. Three biological replicates were set up for each concentration.

[0052] Taking the average value of OD600 of the blank control as a reference, calculate E-2 - Effect of hexenal addition on strain growth. the result shows, E-2 -Hexenal has the effect of promoting the growth of Sphingobacterium strain KSC01, see image 3 .

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Abstract

The invention belongs to the field of astragalus growth-promoting technology, and provides astragalus growth-promoting endophytic bacteria and its growth-promoting method and application in order to solve the current problems of astragalus membranaceus growth promotion and quality improvement. Promoting endophytes are: Sphingobacterium ( Sphingobacterium sp. ) strain KSCO1 and Pseudomonas phrysalis ( Pseudomonas poae ) strain KSCO2; on November 21, 2017, it was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation numbers are: CGMCC No.14945, CGMCC No.14946. 2~10μM E‑2 ‑Hexenal and 5-625 μM n-hexanol promoted the growth of KSC01, and 25-625 μM n-hexanol promoted the growth of KSCO2. A green, environment-friendly pesticide component with no medicinal material residue is provided for the cultivation of Chinese medicinal material Astragalus membranaceus.

Description

technical field [0001] The invention belongs to the field of astragalus growth-promoting technology, and in particular relates to astragalus growth-promoting endophytic bacteria and a growth-promoting method and application thereof. Background technique [0002] Green leaf volatiles (Green leaf volatiles, GLVs) are a class of volatile organic compounds with 6 carbon atoms produced when plants are gnawed by insects, mechanically damaged, or infected by pathogenic bacteria, mainly including: n-hexanal, E - 2 -Hexenal, n-hexanol, etc. [1] . A series of studies on the ecophysiological effects of GLVs have shown that such volatiles not only mediate plant biotic and abiotic stress defense responses, but also promote the accumulation of secondary metabolites such as anthocyanins. [2] , Gueldner and Nakamura and other research groups also found that GLVs also have the functions of inhibiting the reproduction of various pathogenic bacteria and inhibiting the production of aflatoxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02A01P21/00C12R1/01C12R1/38
CPCC12N1/02C12N1/20C12N1/205C12R2001/01C12R2001/38
Inventor 孙海峰孔留利高红柴智郭兰萍高建平
Owner SHANXI UNIV
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