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Sphingobacterium multivorum and application thereof

A technology of sphingosine bacteria and microbial preparations, applied to bacteria, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., to achieve the effect of restoring the self-purification ability of river channels

Active Publication Date: 2019-01-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on facultative heterotrophic nitrification and aerobic denitrification bacteria, and the only few studies are mostly focused on their degradation mechanism

Method used

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  • Sphingobacterium multivorum and application thereof
  • Sphingobacterium multivorum and application thereof
  • Sphingobacterium multivorum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The aerobic fermentation tank of landfill leachate operates stably and the activated sludge of a certain period is used as the separation sludge. On the heterotrophic nitrification medium, the pure Sphingobacterium BT1 strain was isolated by dilution and mixing plate method. The genome of the strain Sphingobacterium BT1 was extracted for 16S rDNA sequencing. The similarity comparison between the sequencing results and the known sequences in the Genbank database showed that the strain Sphingobacterium BT1 had the highest sequence homology with the genus Sphingobacterium, and was identified as Sphingobacterium.

Embodiment 2

[0031] Example 2 Determination of Ammonia Nitrogen Degradation Ability of Sphingobacterium Sphingobacterium BT1

[0032] After activation and enrichment of Sphingobacterium BT1, pipette 1ml of the suspension into 100ml of agar-free heterotrophic nitrification medium, and culture it at 30°C and 160rpm / min for 48 Hour. Take the culture medium every 8 hours. A part of the culture solution was taken to measure the growth curve of Sphingobacterium BT1 at 600nm, and then the remaining culture solution was filtered through a 0.22 μm microporous membrane to detect the indicators of ammonia nitrogen, nitrite nitrogen, nitrate nitrogen and COD in the filtrate. The results are shown in Table 1 and figure 1 shown.

[0033] Table 1 Changes in the degradation rate of ammonia nitrogen by Sphingobacterium BT1

[0034]

[0035] From Table 1OD 600 Index can get the growth curve of the strain. The strain only needs to go through the lag phase of 8 hours to enter the exponential phase. W...

Embodiment 3

[0039] Example 3 Sphingobacterium (Sphingobacterium) BT1 aerobic denitrification capacity determination

[0040] (1) The denitrification performance of Sphingobacterium BT1 was screened and characterized by nitrate medium.

[0041] After activation and enrichment of Sphingobacterium BT1, pipette 1ml of the suspension into 100ml of agar-free nitrate medium or nitrite medium, and culture at 30°C and 160rpm / min , the culture solution was taken every 8 hours to measure the bacterial concentration (OD 600 ), nitrate nitrogen, nitrite nitrogen.

[0042] Table 2 Changes in the degradation of nitrate nitrogen by Sphingobacterium BT1

[0043]

[0044] From Table 2 and figure 2 It can be seen that Sphingobacterium BT1 began to grow at 46 hours and reached the peak at 80 hours. Within 100 hours, nitrate nitrogen dropped from 240.42mg / L to 133.32mg / L, the degradation rate of nitrate nitrogen was 44.5%, and nitrite accumulated from 0 to 40.59mg / L, indicating that Sphingobacterium B...

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Abstract

The invention discloses sphingobacterium multivorum and application thereof, and belongs to the technical field of bioengineering and environmental engineering. The sphingobacterium multivorum is collected in the China General Microbiological Culture Collection Center (CGMCC) on October 17, 2018 at the collection number of CGMCC No. 16596. The strain has heterotrophic nitrification and aerobic denitrification characteristics, can be applied to the field of nitrogen-containing sewage treatment and river treatment, has an ammonia nitrogen removal rate more than 90 percent, is pollution-free to the environment, achieves effects of quickly biologically removing nitrogen and recovering the self-cleaning capacity of a river, and has great significance for the field of environmental water treatment.

Description

technical field [0001] The invention relates to a strain of sphingobacterium and its application, and belongs to the technical fields of bioengineering and environmental engineering. Background technique [0002] Excessive ammonia nitrogen content in rivers and lakes often leads to eutrophication of water bodies and a decrease in dissolved oxygen in water, which has become one of the main causes of water pollution in my country. The ammonia nitrogen in the water body mainly comes from the use of farmland chemical fertilizers, animal feces and sewage produced by the breeding industry, etc. These pollutants enter the lakes and rivers through surface runoff and bring a large amount of nitrate nitrogen and ammonia nitrogen, resulting in deterioration of water quality and damage to the structure of the water ecological environment. . [0003] In the traditional biological denitrification theory, ammonia nitrogen is gradually converted into nitrite nitrogen and nitrate nitrogen b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12N11/10C12N11/14C12R1/01
CPCC02F3/34C02F2101/38C12N1/20C12N11/10C12N11/14C12N1/205C12R2001/01
Inventor 邓敬轩阮文权黄振兴施万胜单晓红
Owner JIANGNAN UNIV
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