Aerobic denitrifying strain and its application
An aerobic denitrifying bacteria and bacillus technology, applied in the field of microorganisms, can solve the problems of harsh screening conditions, high reagent requirements, and complicated steps, and achieve the effect of avoiding environmental discomfort and large application potential.
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Embodiment 1
[0020] Water samples were collected from a depth of 0.5m in Meiliang Bay, Taihu Lake, poured into sterilized sampling bottles, and brought back to the laboratory immediately. Take 100mL of the water samples, add 0.284g of sodium succinate, NaNO 3 10mM, KH 2 PO 4 0.136g, (NH 4 ) 2 SO 4 0.027g, yeast extract 0.1g, MgSO 4 ·7H 2 O 0.019g, trace element solution 0.1mL, adjust pH 7.2, namely SM medium. Put some SM and 1mm sterilized glass beads into a sterilized 500mL Erlenmeyer flask, seal it with a sterilized cotton plug, and place it in a shaker at 30°C and 180rpm for enrichment of good denitrifying bacteria. After culturing for 24 hours, take the bacterial suspension and insert it into fresh sterile SM medium (distilled water configuration) according to 10% inoculum amount, and enrich by continuous passage for three times, and the culture conditions are the same as above. The enriched mixed bacterial suspension was serially diluted and applied to the BTB plate. The fo...
Embodiment 2
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