Aerobic denitrifying strain and its application

An aerobic denitrifying bacteria and bacillus technology, applied in the field of microorganisms, can solve the problems of harsh screening conditions, high reagent requirements, and complicated steps, and achieve the effect of avoiding environmental discomfort and large application potential.

Active Publication Date: 2013-07-24
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have harsh screening conditions, cumbersome steps, and high reagent requirements.

Method used

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  • Aerobic denitrifying strain and its application
  • Aerobic denitrifying strain and its application
  • Aerobic denitrifying strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Water samples were collected from a depth of 0.5m in Meiliang Bay, Taihu Lake, poured into sterilized sampling bottles, and brought back to the laboratory immediately. Take 100mL of the water samples, add 0.284g of sodium succinate, NaNO 3 10mM, KH 2 PO 4 0.136g, (NH 4 ) 2 SO 4 0.027g, yeast extract 0.1g, MgSO 4 ·7H 2 O 0.019g, trace element solution 0.1mL, adjust pH 7.2, namely SM medium. Put some SM and 1mm sterilized glass beads into a sterilized 500mL Erlenmeyer flask, seal it with a sterilized cotton plug, and place it in a shaker at 30°C and 180rpm for enrichment of good denitrifying bacteria. After culturing for 24 hours, take the bacterial suspension and insert it into fresh sterile SM medium (distilled water configuration) according to 10% inoculum amount, and enrich by continuous passage for three times, and the culture conditions are the same as above. The enriched mixed bacterial suspension was serially diluted and applied to the BTB plate. The fo...

Embodiment 2

[0024]

[0025]

[0026]

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Abstract

The invention discloses an aerobic denitrifying strain, which is classified and named as Acinetobacter sp. Strain N2. The strain is preserved in China General Microbiological Culture Collection Center on March 19, 2013, and the preservation number is CCTCC NO: M2013093. The invention also discloses application of the aerobic denitrifying strain. The invention adopts the high efficiency aerobic denitrifying strain screened from a eutrophic lake water body. The strain has a heterotrophic nitrification effect, and can simultaneously remove nitric nitrogen and ammonia nitrogen from the water body. The denitrification process accumulate little nitrite and has no secondary pollution. After expansion culture, the strain can be directly applied to denitrification of lake water, and environment inadaptation of non-indigenous strains can be avoided. Thus, the strain has great application potential in practical engineering.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to an aerobic denitrifying bacterium and its application. Background technique [0002] Aerobic denitrifying bacteria are a type of denitrifying bacteria that use aerobic denitrifying enzymes to perform denitrification under aerobic conditions. In recent years, aerobic denitrifying bacteria have been continuously screened from environmental samples such as wastewater, activated sludge, soil, and farms, confirming the widespread existence of this effect. According to current literature reports, there are three main screening methods for aerobic denitrifying bacteria: (1) Intermittent aeration method: According to the fact that aerobic denitrifying bacteria can simultaneously use O 2 and NO 3 - , frequent switching between aerobic and anoxic is conducive to its advantage in the competition. (2) Selective medium or respiratory inhibitor method: For example, potassium c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 杨柳燕郭丽芸胡志新方菲陈乾坤时飞
Owner NANJING UNIV
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