Sphingobacterium multivolume and method for conversing effective teichomycin by using the same

A technology of Sphingobacterium and Sphingobacterium, which is applied in the field of Sphingobacterium multivorum and its transformation into effective mycin, to achieve the effect of stable performance and low bacterial infection

Inactive Publication Date: 2008-09-10
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Transformation of effective mycin into effective mycylamine and eff

Method used

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  • Sphingobacterium multivolume and method for conversing effective teichomycin by using the same
  • Sphingobacterium multivolume and method for conversing effective teichomycin by using the same

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Embodiment 1

[0028] The formula of the seed medium is as follows (g / L): glucose 10.0, yeast powder 10.0, K 2 HPO 4 7.0, KH 2 PO 4 3.0, MgSO 4 0.001, prepared with tap water, sterilized at 121°C for 30 minutes. Transformation medium formula is as follows (g / L): validamycin 20.0, yeast powder 10.0, K 2 HPO 4 7.0, KH 2 PO 4 3.0, MgSO 4 0.01, prepared with tap water, sterilized at 121°C for 30 minutes. The solid medium, ie the seed medium, was added with 2% agar.

[0029] Cultivation method: Strain CGMCC NO.2341, activated by culturing on solid medium for 2 days at 37°C, inserted into seed medium, cultured at 25°C-37°C, pH 6.8-7.2, cultured under ventilation and stirring conditions, culture time After 24 hours, transfer the cultured seed culture medium to the transformation medium at a rate of 5% of the seeding amount, at a temperature of 25°C-37°C, pH6.8-7.2, and cultivate under ventilation and stirring conditions, and the culture time is 48-168 Hour.

[0030] Product detect...

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Abstract

The invention belongs to the biotechnological field and discloses a Sphingobacterium multivorum and a method for conversion of the Sphingobacterium multivorum into validamycin. The strain or thallus of the strain can convert the validamycin into valid amine and valid enamine; after being cultured in a seed culture medium, the strain or the thallus of the strain is inoculated into a transformation culture medium which contains substrate validamycin for decomposition of the substrate, or the thallus is obtained by separation after being cultured in the seed culture medium and the thallus obtained is added into validamycin solution for decomposition of the substrate; after separation and purification of decomposition liquor, the valid amine and the valid enamine are obtained. The invention also discloses a detection and separation method for the valid amine and the valid enamine. The strain Sphingobacterium multivorum is well-grown in the culture medium which contains the validamycin and the solution culture and transformation effect is good; moreover, the strain has the advantages of innocuity, no pathogenicity, stable performance, difficulty in contamination and so on.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a Sphingobacteriumm ulvorum and a method for transforming effectivemycin, in particular to screening and isolating a strain of Sphingobacterium ulvorum capable of degrading effectivemycin from soil The invention relates to Sphingobacterium polyphagous of the genus Sphingobacterium, a method for transforming effective mycin into effective mycamine and effective mycylamine by using the strain, and a method for separating and detecting effective mycamine and effective mycylamine. Background technique [0002] The present invention relates to valid mycamine (validamine) and valid mycylamine (valiednmine), structural formula is as follows: [0003] [0004] Valid amine (validamine) Valid amine (valienamine) [0005] Effective mycoamine, the molecular formula is C 7 h 15 NO 4 , the important groups are: a primary amino group (-NH 2 ), a hydroxymethyl group (-CH 2 OH), three hydroxyl...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00C12R1/01
Inventor 张怡轩吴春福
Owner SHENYANG PHARMA UNIVERSITY
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