Noni sphingomonas xenophaga preserving culture medium and preparation method thereof
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The technology of sphingomonas and noni sphingosine is applied in the field of preservation medium of noni sphingomonas and its preparation, and can solve the problem that sphingomonas is late and research stays in the primary stage , ecological value and economic value are rarely paid attention to
Inactive Publication Date: 2016-04-20
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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Problems solved by technology
However, due to the late understanding of Sphingomonas, the ecological value and economic value of this...
Method used
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Embodiment 1
[0011] The formula of the culture medium is: 0.8% dry powder of nutrient broth, dilute to 1000ml with 1% noni dried fruit aqueous solution, and adjust the pH to 4.0-6.0.
[0012] Take 10.0g noni dried fruit, add 1000ml water to boil for 10min, add water to 1000ml, add 8.0g nutrient broth dry powder, boil to dissolve, adjust pH to 4.0-6.0, then subpackage and sterilize at 121°C for 20min.
[0013] Use an inoculation loop to pick a loop from the seed slant to inoculate, and store it in a 4°C refrigerator after culturing for 2 days. Take it out after storage for 70 days, the culture medium is brown-black, and a large amount of off-white bacteria is deposited on the bottom of the test tube. After shaking well, dip an inoculation loop bacteria solution and streak it onto a fresh solid medium. After 2 days of cultivation, off-white colonies grow on the streaked place It shows that the Sphingomonas noni preserved in this medium still has activity.
Embodiment 2
[0015] The formula of the culture medium is: dry powder of nutrient broth, 0.8% agar, 0.5%, dilute to 1000ml with 5% noni dried fruit aqueous solution, and adjust the pH to 4.0-6.0.
[0016] Take 50.0g of noni dried fruit, add 1000ml of water and boil for 10min, add water to 1000ml, add 8.0g of nutrient broth dry powder and 5.0g of agar, boil and dissolve, adjust the pH to 4.0-6.0, then subpackage and sterilize at 121°C for 20min.
[0017] Use an inoculation needle to pick the bacteria from the seed slope for puncture inoculation. After 2 days of cultivation, the bacteria can be seen growing along the puncture line, and then stored in a refrigerator at 4°C. After 70 days of storage, take it out, use an inoculation loop to take the thallus on the puncture line and streak it onto a fresh solid medium, and grow gray-white colonies after 2 days of cultivation, indicating that the thallus is still active.
[0018] The preservation culture medium prepared by the method of Embodiment...
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Abstract
The invention discloses a noni sphingomonas xenophaga preserving culture medium. The culture medium is prepared from 0.8% of dry powder of nutrient broth, 0-0.5% of agar and 1-5% of a noni dried fruit aqueous solution, wherein the volume of the noni dried fruit aqueous solution is settled to be 1000ml, and the pH is adjusted to be 4.0-6.0; the preparation method of the 1-5% of noni dried fruit aqueous solution comprises the following steps of taking 10.0-50.0g of dried fruits, adding 1000ml of water, boiling over the dried fruits and the water for 10 minutes, and replenishing water to 1000ml. After the noni sphingomonas xenophaga cultured on a traditional nutrient agar culture medium is preserved for 15d, the phenomenon that the preserved noni sphingomonas xenophaga is inactive during inoculation appears. According to the preserving culture medium disclosed by the invention, the preserving time of the noni sphingomonas xenophaga can be prolonged, the longest preservation time can achieve 70d, compared with the preservation time of an ordinary nutrient agar culture medium, the preservation time of the noni sphingomonas xenophaga preserving culture medium is prolonged by 3.7 times, and the purpose of long-term preservation is achieved.
Description
technical field [0001] The invention relates to the field of preservation of microbial strains, in particular to a preservation medium for Sphingomonas noni and a preparation method thereof. Background technique [0002] Noni (Morinda officinalis) is one of the most important plants brought to Hawaii by the early Polynesians, and it is the most commonly used Chinese medicinal material by ancient Hawaiians. Noni and its related extracts play an active role in anti-tumor, improving immune regulation function, reducing liver damage and so on. Especially the use of noni fruit alone or in combination with other medicines as a remedy for many ailments such as diabetes, hypertension and cancer treatment. Scientific studies have confirmed that noni juice can prolong the lifespan of mice with Lewis lung cancer and inhibit the growth of tumor cells by stimulating the immune system. The noni juice alcohol precipitate (Nonippt) has anticancer and antibiotic activity. However, the cur...
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IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/205C12R2001/01
Inventor 杨娟都海渤刘洋陈建国李辉梁寒峭程池吴甡崔楚民闻红左山
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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