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Sphingomonas strain xp and application of sphingomonas strain xp to degradation of phenolic compounds in tobacco products

A kind of technology of polyphenolic compound and sphingosine unit cell, applied in the field of microorganisms

Active Publication Date: 2014-04-30
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the content of polyphenolic compounds exceeds a certain range, the tobacco leaves have various quality defects in varying degrees, such as: green miscellaneous and irritating, monotonous aroma, insufficient aroma, etc., and cannot be directly used to produce cigarettes
More importantly, excessive polyphenol compounds are toxic to the human body and will affect the health of smokers

Method used

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  • Sphingomonas strain xp and application of sphingomonas strain xp to degradation of phenolic compounds in tobacco products
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  • Sphingomonas strain xp and application of sphingomonas strain xp to degradation of phenolic compounds in tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 : Determination of the ability of Sphingomonas xp to utilize chlorogenic acid for growth

[0037] 1) Preparation of seed culture medium: Resuscitate the strain xp with LB medium, then inoculate it into inorganic salt medium containing 0.5g / L chlorogenic acid, culture it on a shaker at 30°C for 24 hours (rotation speed 200rpm), and collect the bacteria by centrifugation , washed 3 times with inorganic salt medium, then resuspended the cells with inorganic salt medium, adjusted to OD 600 When the value is 3.0, the resulting suspension is the seed culture solution.

[0038] 2) The growth ability of Sphingomonas: Add the seed culture solution to the inorganic salt medium to obtain the initial OD 600 value of 0.15, then add 0.5g / L chlorogenic acid, take a sample at 48h, detect the turbidity of the bacterial liquid and the content of chlorogenic acid (the content of chlorogenic acid is determined by high performance liquid chromatography), the results are shown...

Embodiment 2

[0040] Example 2: Determination of the ability of Sphingomonas xp to grow using tobacco extract

[0041] 1) Preparation of tobacco extract: Weigh 1g of flue-cured tobacco leaves, crush them, add 20ml of 60% ethanol, extract in a water bath at 50°C for 50 minutes, shake well every 10 minutes, filter after extraction, and the filtrate is the tobacco extract.

[0042] 2) Preparation of seed culture medium: resuscitate the strain xp with LB medium, then inoculate it into the inorganic salt medium containing 0.1 ml / L tobacco extract, cultivate it on a shaker at 30°C for 24 h (rotation speed 200rpm), and collect the bacteria by centrifugation cells, washed 3 times with inorganic salt medium, then resuspended the cells with inorganic salt medium, adjusted to OD 600 When the value is 3.0, the resulting suspension is the seed culture solution.

[0043] 3) The growth ability of Sphingomonas: Add the seed culture solution to the inorganic salt medium to obtain the initial OD 600 valu...

Embodiment 3

[0045] Example 3: After the Sphingomonas strain xp was cultivated with chlorogenic acid as the sole carbon source, the determination of the degradation ability of polyphenols in flue-cured tobacco leaves and the influence on the taste of tobacco leaves

[0046] 1) Preparation of seed culture solution: the preparation method is the same as in Example 1.

[0047] 2) Degradation ability of Sphingomonas xp: 50ml seed culture solution was evenly sprayed on the surface of 500g flue-cured tobacco leaves at 30°C and humidity 60%, and samples were taken at different times to detect the content of polyphenols in flue-cured tobacco leaves. For the control group, 50ml of distilled water was evenly sprayed on the surface of 500g of flue-cured tobacco leaves. The tobacco leaves used in the experiment were selected from the 2009 tobacco leaves in Jia County, Henan Province.

[0048] The polyphenol content was determined by the Folin phenol method: 1g of tobacco powder was put into a centr...

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Abstract

The invention belongs to the technical field of microbial strains and in particular relates to a sphingomonas strain xp. The sphingomonas strain xp is classified and named as Sphingomonas sp., and the collection number of the strain in the China center for type culture collection (CCTCC) is CCTCC No:M 2011453. The invention further relates to an application of the sphingomonas strain xp to degradation of phenolic compounds in tobacco products, and the degradation effect is good.

Description

[0001] technical field [0002] The invention belongs to the technical field of microorganisms, and in particular relates to a sphingomonas strain xp and its application in degrading polyphenol compounds in tobacco products. Background technique [0003] Sphingomonas is widely distributed in the environment and has strong tolerance to harsh environments (for example, Sphingomonas has been found in extreme environments such as polar regions and strong radiation). Sphingomonas can degrade a wide range of substrates, such as the degradation of aromatic compounds such as dioxins, phenols, azo dyes, and heterogeneous biomass polymers. Because Sphingomonas has a very wide range of metabolic capabilities for aromatic compounds, and some species of this genus can synthesize valuable extracellular biopolymers, Sphingomonas has become a concern in recent years. and research hotspots. [0004] Tobacco polyphenols are aromatic ring compounds with one or more hydroxyl groups synthesized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20C12R1/01
Inventor 马宇平周浩戴建国苏东赢王墨染唐鸿志
Owner CHINA TOBACCO HENAN IND
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