3results about How to "High polysaccharide yield" patented technology

The invention relates to the technical field of biological medicine, and particularly discloses a polysaccharide content detection method and application. The polysaccharide content detection method provided by the invention comprises the following steps: preparing a solution, drawing a standard curve and detecting a sample, the step of preparing the solution comprises preparation of a standard solution, preparation of a standard serum diluent and preparation of a sample diluent; wherein the specific steps of preparing the standard serum diluent are as follows: diluting polysaccharide standard serum by 3 times with a buffer solution to obtain the standard serum diluent; the preparation method of the sample diluent comprises the following specific steps: centrifuging a sample to be detected at 8000-10000 g for 15-30 minutes, taking supernate, and diluting to obtain the sample diluent. The detection method provided by the invention has the advantages of simplicity in operation, high sensitivity, good repeatability and the like, and can provide powerful support for culture condition optimization in a pneumococcus production and fermentation process and quality control of pneumococcus polysaccharide vaccines.

The invention discloses a method for improving mycelium yield, polysaccharide yield and polysaccharide efficacy in liquid fermentation of pilose antler mushrooms, and belongs to the technical field of liquid fermentation of pilose antler mushrooms. The radix astragali aqueous extract is added into the pilose antler mushroom liquid fermentation culture medium, so that the growth speed of pilose antler mushroom hyphae is effectively promoted, the hypha yield is increased, the liquid fermentation time is shortened, meanwhile, the yield and titer of pilose antler mushroom polysaccharides can be increased, and the problems that the pilose antler mushroom liquid fermentation time is long, and the fermentation effect is poor are solved. The efficiency of extracting the antler mushroom polysaccharide from the sporocarp is relatively low; the functional titer of the antler mushroom polysaccharide is relatively low; and the development of the antler mushroom planting industry is limited.

This invention relates to the field of microbial fermentation technology, and particularly to *Phellinus linteus* mycelium, a method for increasing the polysaccharide yield of *Phellinus linteus* mycelium, and their applications. The method involves placing *Phellinus linteus* mycelium in an alternating magnetic field environment with a magnetic field strength of 2 mT to 20 mT and a frequency of 2 Hz to 100 Hz during the late logarithmic growth phase to the early stationary phase of its cultivation. Compared to a control group without magnetic field treatment, this method can increase the intracellular polysaccharide yield of *Phellinus linteus* mycelium by 10%–95%, while maintaining stable or slightly increased mycelial biomass. Furthermore, this method is a purely physical stimulation, introducing no chemical substances into the fermentation system, resulting in no pollution or residue, and aligns with the development direction of green biomanufacturing.