34results about How to "High polysaccharide yield" patented technology

The invention discloses a Grifola frondosa strain for producing polysaccharide with a composite raw material of rice bran and wheat bran, which belongs to the technical field of microbial application technology and food biology. The Grifola frondosa strain JSU10 is preserved in China general microbiological culture collection center (CGMCC) in October 8th, 2010 with the CGMCC No. 4179 and is identified to be Grifolasp. The invention improves the positive mutation rate of the Grifola frondosa strain for rapidly growing and producing Grifola frondosa polysaccharide on a composite culture medium of rice bran and wheat bran by composite mutagenesis of ultraviolet rays and microwave to obtain a high-yield strain; the strain and the original strain are respectively a liquid fermentation rice bran and wheat bran composite culture medium; and hypha dry weight and polysaccharide of the mutated strain are respectively improved by 39.24 percent and 42.58 percent compared with the original strain.

The invention belongs to the fields of microbe application technologies and food biotechnologies, and discloses a strain used for fermenting rice bran and wheat bran extracts for producing grifolan. The grifola frondosa strain is collected in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan, China on Aprial 7th, 2011, and has a strain collection number of CCTCC No: M2011113. The name of the strain is Grifolasp. JSU10-2. According to the invention, through protoplast laser mutation, the strain with high yield of mycelium polysaccharide produced from cheap raw materials is obtained. When the strain and an original strain are respectively used in liquid fermentation of a rice bran and wheat bran composite culture medium, the dry weight and polysaccharide of the mutant strain are respectively increased by 31.7% and 32.6% compared with those of the original strain.

The invention belongs to the technical field of microbe application and good bioscience and discloses a polysaccharide grifola strain produced through a rice bran and wheat bran complete feed liquid culture medium. grifola Grifola sp.JSU1301 is preserved in the China Typical Model Cultivation Center (CCTCC) in June 25th, 2013, and is numbered CCTCC NO: M 2013286 and named a grifola Grifola sp.CCTCC NO: M 2013286. According to the invention, a ultraviolet mutagenesis method is described with figures; a new strain capable of highly producing polysaccharide by starting with Grifola sp.CCTCC NO: M 2011113; the new strain quickly ferments and highly produces polysaccharide in the rice bran and wheat bran complete feed liquid culture medium; the mycelial dry weight and the mycelial polysaccharide productivity of a shake flask are increased by 16.8% and 8.57% compared with those of a starting strain, and those of fermentation in a tank are improved by 30.9% and 29.7%, which reach the highest level at present.

The invention discloses an extraction method of wolfberry polysaccharide, comprising the following steps: A picking wolfberry; B grinding the fresh wolfberry into homogeneous pulp using a pulp grinder; C pouring the homogeneous pulp into a container; D microwave processing the homogeneous pulp to 50-100 centigrade and boiling the homogeneous pulp for 10-60 minute; E stopping the microwave processing and adding the purified water into the boiled pulp and continuously stirring up the pulp until the temperature is reduced to 50-80 centigrade; F inserting an ultrasonic rod to perform ultrasonic treatment for 20-90 minute; G processing the pulp being subjected to ultrasonic treatment using an industry centrifuges at 3000-5000 r and spilling out the supernatant and decompression concentrating H at 60-90 degree and adding alcohol until the final alcohol concentration is 80-90% and separating and precipitating the mixture using the industry centrifuges at 3000-5000 r to obtain the polysaccharide raw powder or coarse paste. The extraction method has features of high efficiency, energy saving and quick extraction speed. The extraction method solves the problem that the work for drying the wolfberry is heavy and the energy source is waste in Northwest China in harvest season and the extraction technology of the wolfberry polysaccharide is increased and the wolfberry polysaccharide yield is increased.

The invention discloses a preparation method of carboxymethyl pachyman, and particularly provides a method for fermentation preparation of carboxymethyl pachyman by regulating the variety and proportion of a carbon source and a nitrogen source in a Tuckahoe culture medium. Consisting of the processes of Tuckahoe strain activation, seed solution preparation, fermentation cultivation and carboxymethyl pachyman extraction, the preparation method is characterized in that in the process of fermentation cultivation, the fermentation culture medium comprises the following components by weight percentage: the carbon source (2.0%-4.5% of glucose, 0.1%-4.5% of carboxymethyl cellulose), the nitrogen source (0.45%-0.65% of yeast extract and 0.35%-0.55% of peptone), 0.1% of K2HPO4, 0.046% of KH2PO4, 0.05% of MgSO4.7H2O, and the balance water. The method provided in the invention can directly obtain carboxymethyl pachyman with certain degree of substitution and no chemical residue, and the carboxymethyl pachyman can be widely used in medicine, food and other fields.

The invention relates to Xanthomonas sp. NYW79 and use thereof. NYW79 strain is used to produce non-pigmented xanthan gum via the following steps such as activation culturing, liquid seed culturing, fermentation culturing and xanthan gum precipitating. The yield of xanthan gum production using the NYW79 strain is higher than 14.9% as high as that by induction of starting NY07 strain; OD value of fermentation broth is decreased by higher than 77.1%, the production of xanthan gum with NYW79 strain can substantially solve the fading problem of xanthan gum products, and xanthan gum polysaccharide yield is significantly increased.

The invention provides an additive for a liquid nutrient medium for Irpex lacteus fermentation, relating to formula optimization of a liquid nutrient medium for Irpex lacteus fermentation. Calcium lignosulphonate and natural borneol are added into the broad liquid nutrient medium to enhance the transport efficiency of Irpex lacteus cells, and promote the absorption and utilization of the Irpex lacteus for nutrient substances, thereby enhancing the yield of the cellular metabolism products adenosin and polysaccharide and the dry weight of hypha. The total adenosin content is up to 0.135-0.149 g/L, which is enhanced by 8.2% on average as compared with the liquid nutrient medium without the additive; the total polysaccharide content is up to 2...381-2.605 g/L, which is enhanced by 11.0% on average as compared with the liquid nutrient medium without the additive; and the dry weight of hypha is up to 15.176-15.320 g/L, which is enhanced by 9.1% on average as compared with the liquid nutrient medium without the additive. The additive can be used as a liquid nutrient medium additive for industrially fermenting Irpex lacteus.

The invention discloses a culture medium for screening a strain capable of highly yielding a Hib vaccine. The formula of the culture medium comprises the following components: a yeast extract, disodium hydrogen phosphate, sodium dihydrogen phosphate, a 60% sodium lactate solution, magnesium chloride, calcium chloride dihydrate, ammonium sulfate, glucose, chlorhematin, a coenzyme I and/or agar. A preparation method of the culture medium for screening the strain capable of highly yielding the Hib vaccine comprises the steps of weighting, preparing a solution A, preparing a solution B and mixing. The high-yielding strain with a capsule is screened by virtue of carrying out passage growth on solid and liquid culture media for many times. Since the culture medium disclosed by the invention is free of animal blood and protein components derived from animals, the potential risk is avoided and the safety of the vaccine is improved; the preparation method of the culture medium has the advantages of simple operation, convenience in preparation and low cost; due to the screening method, the problem of strain screening in the scale production of the Hib vaccine is solved, the yield of the Hib capsular polysaccharide is greatly increased, the working efficiency is improved and the production cost is decreased.

The invention discloses a method for preparing and purifying grifola frondosa polysaccharide with high in-vitro antioxidant activity. According to the method, farnesol is added at beginning of or in a process of liquid fermentation of grifola frondosa. According to the method, farnesol is added in the liquid fermentation process of grifola frondosa mycelia, so that polysaccharide yield of grifola frondosa fermentation liquor is promoted to be changed, yield of acidic polysaccharide components generated by grifola frondosa fermentation is remarkably increased, the polysaccharide yield of the grifola frondosa fermentation liquor is remarkably increased on the basis of not increasing an original fermentation period, the maximum increase amplitude reaches 149%, content of the separated and purified acidic polysaccharide is increased from 7.4% to 58.2%, the in-vitro antioxidant activity of the separated and purified acidic polysaccharide is remarkably improved compared with that of a control group, and the exogenous additive farnesol is safe, non-toxic and high in stability, so that a foundation is laid for industrial production of polysaccharide in the grifola frondosa fermentation liquor.

The invention discloses a method for extracting low-molecular-weight kelp fucoidin, and belongs to the technical field of extraction of polysaccharide. The method for extracting the kelp fucoidin comprises the following steps: cell wall cracking, polysaccharide rough extraction, and polysaccharide extraction, separation and purification. The step of cell wall cracking is as follows: taking 10-16 parts by weight of fucus powder, soaking the fucus powder with anhydrous ethanol for degreasing, extracting solid residues with water under the condition that the solid-liquid ratio is 1: 10-15 m/V, stirring for 20-35 min, standing for 4-6 h in a sealed manner, adding active peptide, stirring for 5-8 min, carrying out steam blasting treatment, and maintaining pressure at 2-2.8 MPa for 30-40 S to obtain cell breakage liquid. The breakage rate of the kelp fucoidin is increased by the active peptide, the yield and purity of the fucoidin are improved, the extracted fucoidin is low in molecular weight, easy to absorb and good in anti-tumor and health-care effect, and the preparation cost of the fucoidin is low.

The invention discloses an application of dendrobium polysaccharide in the preparation of drugs for prevention and/or treatment of benign prostatic hyperplasia. The dendrobium polysaccharide has obvious therapeutic effect and no toxic and side effects, which can improve the antioxidant capacity of prostate and testes, restore normal expression of key genes of steroid synthesis in leydig cells of benign prostatic hyperplasis, inhibit the expression of 5 alpha-reductase gene in the benign prostatic hyperplasia tissue and effectively treats benign prostatic hyperplasia, especially benign prostatic hyperplasia induced by exogenous androgen. The dendrobium polysaccharide with wide source of raw materials is simple in extraction and purification method, high in sugar content and suitable for large-scale production and popularization and has a good prospect in the preparation and/or treatment of prostate hyperplasia.