34results about How to "High polysaccharide yield" patented technology

The invention discloses a preparation method of carboxymethyl pachyman, and particularly provides a method for fermentation preparation of carboxymethyl pachyman by regulating the variety and proportion of a carbon source and a nitrogen source in a Tuckahoe culture medium. Consisting of the processes of Tuckahoe strain activation, seed solution preparation, fermentation cultivation and carboxymethyl pachyman extraction, the preparation method is characterized in that in the process of fermentation cultivation, the fermentation culture medium comprises the following components by weight percentage: the carbon source (2.0%-4.5% of glucose, 0.1%-4.5% of carboxymethyl cellulose), the nitrogen source (0.45%-0.65% of yeast extract and 0.35%-0.55% of peptone), 0.1% of K2HPO4, 0.046% of KH2PO4, 0.05% of MgSO4.7H2O, and the balance water. The method provided in the invention can directly obtain carboxymethyl pachyman with certain degree of substitution and no chemical residue, and the carboxymethyl pachyman can be widely used in medicine, food and other fields.

The invention belongs to the field of microorganism application technology and food biotechnology, and discloses a bacterial strain for fermenting rice bran and bran extract to produce grifola frondosa polysaccharide. The grifola frondosa strain has been preserved in the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China on April 7, 2011. The preserved strain number is CCTCC No: M2011113, and the name is Grifolasp.JSU10-2. The present invention obtains a bacterial strain that utilizes cheap raw materials to produce high-yield mycelia polysaccharides through protoplast laser mutagenesis. The bacterial strain and the original strain respectively liquid-ferment rice bran and bran compound medium, and the mycelium dry weight and polysaccharide of the mutagenic strain are respectively higher than those of the original The strains improved by 31.7% and 32.6%.

The invention discloses the application of dendrobium polysaccharides in the preparation of drugs for preventing and / or treating hyperplasia of the prostate gland. The dendrobium polysaccharide has obvious therapeutic effects, no toxicity and side effects, can improve the antioxidant capacity of the prostate and testis, restore the normal expression of key genes for steroid synthesis in Leydig cells of benign prostatic hyperplasia, and inhibit 5α-reductase in benign prostatic hyperplasia tissue Gene expression, thereby effectively treating benign prostatic hyperplasia; especially benign prostatic hyperplasia caused by exogenous androgen. The Dendrobium polysaccharide of the present invention has wide sources of raw materials, simple extraction and purification method, high sugar content of the obtained Dendrobium polysaccharide, is suitable for large-scale production and popularization, and has good performance in the preparation of drugs for preventing and / or treating prostatic hyperplasia and clinical application. prospect.

The invention discloses a Naoxinshu oral liquid and a preparation method thereof. The preparation method comprises the following steps: S1, preparation of Armillaria concentrated solution: S11, inoculating Armillaria strains into fermentation culture liquid, fermenting and culturing , to obtain a fermented liquid; S12, inactivate the fermented liquid obtained in step S11, filter and concentrate to obtain a concentrated liquid of Armillaria armillaria; S2, heat and refine the honey, filter, let it cool, and mix it evenly with the ground royal jelly, Sieve, then add Armillaria concentrated solution prepared in step S1, sodium benzoate aqueous solution and vanilla essence ethanol solution in sequence, mix evenly, add purified water to volume, stir evenly, filter, and obtain; at the same time, protect the brain extract prepared by the above method Xinshu Oral Liquid. The invention has short fermentation period, good bacterial growth, high polysaccharide content and stable quality, improves the drug efficacy and quality stability of the product, can meet clinical needs, and the prepared product has better hypoglycemic effect.

The invention discloses a method for producing exopolysaccharides by promoting fermentation of lucid ganoderma liquid, and belongs to the field of microorganism fermentation. According to the method, liquid shallow fermentation of lucid ganoderma is adopted, the liquid level height of a fermentation culture medium is controlled to be 0.1-5cm, and the yield of ganoderan can be increased. The method has a relatively short fermentation cycle in comparison with solid-state fermentation, does not strictly need stirring, ventilating and other devices in comparison with liquid-state fermentation, the equipment and production cost can be reduced, the polysaccharide yield of unit thallus can be remarkably increased, and 0.086g / g thallus is increased to 0.243g / g thallus, which is increased by 1.83 times, so that industrial production is benefited.

The invention discloses a preparation and purification method of Grifola frondosa polysaccharide with high antioxidant activity in vitro. In the method of the invention, by adding farnesol in the liquid fermentation process of Grifola frondosa mycelium, the polysaccharide yield of the fermentation broth of Grifola frondosa is changed, the yield of acidic polysaccharide components produced by the fermentation of Grifola frondosa is remarkably increased, and the original polysaccharide component is not increased. On the basis of the fermentation cycle, the polysaccharide yield of the fermentation broth of Grifola frondosa was significantly improved, with a maximum increase of 149%, and the content of acidic polysaccharides after separation and purification increased from 7.4% to 58.2%. Compared with the control group, the oxidative activity was significantly improved, and the exogenous additive farnesol was safe, non-toxic and highly stable, thus laying the foundation for the industrial production of polysaccharides in the fermentation broth of Grifola frondosa.

The invention relates to the cultivation and accumulation of biomass and polysaccharides of spirulina, in particular to the cultivation of spirulina by controlling the addition of nutrient salts so that the biomass and polysaccharides can be accumulated simultaneously. The spirulina cells cultivated to the exponential growth phase are transferred to the nutrient-limited medium, with natural light or artificial light, and the algae cells are harvested after being cultivated to the stable phase. The yield is 0.8-20 times higher than that under the condition of rich nutrition, and the polysaccharide content reaches 45%-80% of the dry weight of algae, which is 2-6 times higher than that under the condition of rich nutrition. The invention solves the contradiction that spirulina biomass and polysaccharides cannot be accumulated at the same time, realizes the rapid and efficient production of spirulina polysaccharides, has the advantages of less nutrient salt consumption, low production cost, high polysaccharide content, etc., and is conducive to simplifying downstream processing operations and promoting spirulina Industrial production of algal polysaccharides.

The invention discloses a pigment-free low-molecular weight Welan gum production strain. Sphingomonas sp. HT-1 (CCTCC NO: M2012062) is taken as an original strain to respectively construct a pigment-deficient and decapsulated-structure sphingomonas genetically engineered strain WG-1, a pigment and degrading enzyme gene-deficient sphingomonas engineered strain WG-2, and a pigment and degrading enzyme gene-deficient and decapsulated-structure sphingomonas genetically engineered strain WG-3. The yield of the Welan gum produced by the sphingomonas strain WG-2 in the invention is increased by 10% to 30% compared with the original strain, reaching 35 to 45g / L, and the molecular weight is not significantly changed (Mn: 10000 to 20000kDa). The sphingomonas strain WG-1 and the sphingomonas strain WG-3 in the invention are fermented to produce low-molecular weight Welan gum (Mn: 500 to 1000kDa), and the yield of the Welan gum produced by the decapsulated-structure strain WG-3 is increased by 20%to 30% compared with the strain WG-1, reaching 20 to 25g / L. The strain constructed by the invention can obtain Welan gum products of different molecular weight ranges, and significantly improves thefermentation level of Welan gum.

The invention relates to a production method of a grifolan selenium compound, and relates to the food microbiological application technology field. The method comprises the steps that: rice bran and wheat bran are leached in water with a temperature of 90 DEG C to 98 DEG C for 2.5-3h under normal pressure; residuals are removed, and a juice is obtained; the obtained process liquid is used as a culture media for fermentation, wherein the rice bran application amount is 30-120g / L culture media, the wheat bran application amount is 30-120g / L culture media, an addition amount of selenium is 5-10mg / L culture media, an addition amount of potassium dihydrogen phosphate is 1.0-1.5g / L, an addition amount of magnesium sulfate is 0.5-0.80g / L, and a pH value is natural; mycelium obtained by liquid culturing is subjected to centrifugal separation, and is washed by using distilled water; the mycelium is dried to constant weight, such that the mycelium is obtained; the mycelium is subjected to conventional water extraction, deproteinization, ethanol precipitation, and lyophilization, such that a intracellular polysaccharide selenium compound can be obtained. With the method provided by the invention, the production of grifolan selenium compound by using cheap raw materials is better realized.

The invention belongs to the fields of microorganism application and food biotechnology and discloses a method for producing polysaccharide by rice husk bran composite raw material and grifola frondosa mutant strain. The method comprises the following steps of: (1) treating rice husk and bran by adopting plant hydrolytic enzyme; (2) fermenting a grifola frondosa strain obtained by mutagenizing with the obtained treating fluid as a culture medium, wherein the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.4179; (3) carrying out centrifugal separation on mycelia obtained by liquid culture, washing the mycelia with distilled water, and drying to be constant weight to obtain the mycelia; (4) carrying out the conventional water extraction, deproteinization and ethanol precipitation on the mycelia to obtain intracellular polysaccharide; and (5) sequentially carrying out deproteinization and ethanol precipitation on the supernate obtained after fermentation liquor is centrifuged to obtain intracellular polysaccharide. By adopting the method disclosed by the invention, the production cost can be reduced, the material cost can be saved, less land can be used, the production cycle can be shortened, and grifola frondosa polysaccharide with high value and auxiliary anti-tumor curative effect can be produced.