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Method for producing polysaccharide by rice husk bran composite raw material and grifola frondosa mutant strain

A technology for mutagenizing strains and Grifola frondosa, applied in the directions of fungi, fermentation, etc., can solve problems such as no reports, and achieve the effects of reducing production costs, high growth rate, and reducing production cycle.

Inactive Publication Date: 2013-04-17
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another key problem of the present invention is to obtain the technological method of fermenting high-yield Grifola frondosa polysaccharides on rice bran bran composite medium by using mutagenic strains, and this research has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The strain of Grifola frondosa was the JSU10 strain obtained by mutagenesis. Use the plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 34U / g raw material, use a solid-liquid ratio of 1:15, and enzymatic hydrolysis time is 12h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 3g / 100mL medium, the amount of bran used is 3g / 100mL medium, add potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH is natural, the sample volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 26 o C, rotation speed 150r / min, culture time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at...

Embodiment 2

[0017] Use plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 38FPU / g raw material, use a solid-liquid ratio of 1:25, and enzymatic hydrolysis time is 18h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 11g / 100mL medium, the amount of bran used is 11g / 100mL medium, add potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH natural, the sample loading volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 28 oC , rotation speed 180r / min, culture time 9d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a cert...

Embodiment 3

[0019] Use the plant hydrolase Viscozyme L produced by Novozymes, Denmark, with an enzyme activity of 1590.41IU / mL (FPU enzyme activity), add enzyme at 35FPU / g raw material, use a solid-liquid ratio of 1:18, and enzymatic hydrolysis time of 14h for rice bran Treat with bran to obtain the treatment solution as a medium for fermentation, the amount of rice bran used is 9g / 100mL medium, the amount of bran used is 5g / 100mL medium, and potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 0.75 g / L, pH natural, the sample loading volume of the shake flask is 40% volume, the inoculum volume is 10%, and the culture temperature is 28 oC , rotation speed 150r / min, culture time 7d. The mycelia obtained from liquid culture were centrifuged and washed 3 times with distilled water to remove the culture medium adhering to the surface of the mycelium, and then put into a blast drying oven and dried to constant weight at 60°C. The dry weight of mycelium was obtained by weighing. Add a ce...

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PUM

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Abstract

The invention belongs to the fields of microorganism application and food biotechnology and discloses a method for producing polysaccharide by rice husk bran composite raw material and grifola frondosa mutant strain. The method comprises the following steps of: (1) treating rice husk and bran by adopting plant hydrolytic enzyme; (2) fermenting a grifola frondosa strain obtained by mutagenizing with the obtained treating fluid as a culture medium, wherein the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.4179; (3) carrying out centrifugal separation on mycelia obtained by liquid culture, washing the mycelia with distilled water, and drying to be constant weight to obtain the mycelia; (4) carrying out the conventional water extraction, deproteinization and ethanol precipitation on the mycelia to obtain intracellular polysaccharide; and (5) sequentially carrying out deproteinization and ethanol precipitation on the supernate obtained after fermentation liquor is centrifuged to obtain intracellular polysaccharide. By adopting the method disclosed by the invention, the production cost can be reduced, the material cost can be saved, less land can be used, the production cycle can be shortened, and grifola frondosa polysaccharide with high value and auxiliary anti-tumor curative effect can be produced.

Description

Technical field [0001] The present invention involves the field of food microbial application technology, especially the use of a medium -to -be -growing gray tree -based altering strain suitable for the medium that is suitable for rice bran and bran composite to grow and high -yield polysaccharides. Background technique [0002] Scientific research and domestic markets at home and abroad have shown that edible bacteria polysaccharides can enhance human immunity and auxiliary treatment of tumor diseases, which has become auxiliary items for tumor biological therapy.Domestic medicines made of edible fungus entities include Ganoderma Capsules in Wuhu, Anhui, Gray Tree Flower Capsules in Qingyuan, Zhejiang. Non -drug products made from edible fungus extraction are also sold.New Zealand has a "GANOPOLY" (Ganno Pilli) polysaccharide extract series products, which are sold to the United States, Australia, China (including Hong Kong and Taiwan) and other places.The inventor of this prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12N1/14
Inventor 刘伟民张建郭春梅徐立平任晓锋张志才顾慧敏杨锁华赵杰文
Owner JIANGSU UNIV
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