A kind of Naoxinshu oral liquid and preparation method thereof

A technology of Naoxinshu oral liquid and concentrated liquid, which is used in pharmaceutical formulations, dispersion liquid delivery, cardiovascular system diseases, etc., can solve the problems of long fermentation period, low content of active ingredients, unstable quality, etc. growth, increase polysaccharide production, and meet clinical needs

Active Publication Date: 2021-10-22
GUANGDONG YILI LUODING PHARMA
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Problems solved by technology

[0011] But at present, the fermentation yield of armillaria concentrated solution is low and the production process is unstable, resulting in extremely unstable product quality, which is difficult to meet the needs of product quality control, drug efficacy and clinical effect
CN107213172A discloses a preparation method of Naoxinshu oral liquid. Armillaria armillaria concentrated liquid is prepared by a three-stage fermentation method, which can reduce polypeptide impurities and increase active ingredient N6-(5-hydroxyl-2-pyridylmethylamino) The content of -9-β-purine nucleoside improves the above problems, but it still has problems such as long fermentation period, low yield of fermented liquid and low content of active ingredients such as polysaccharides, resulting in poor efficacy and unstable quality of Naoxinshu Oral Liquid , it is difficult to meet the clinical needs

Method used

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  • A kind of Naoxinshu oral liquid and preparation method thereof
  • A kind of Naoxinshu oral liquid and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0038] A preparation method of Naoxinshu oral liquid, comprising the following steps:

[0039] S1, the preparation of armillaria concentrated solution:

[0040] S11. Inoculate the Armillaria strain into the fermentation broth at a temperature of 26-28°C and a tank pressure of 0.05-0.08×10 5 Pa, fermented and cultivated for 3-5 days to obtain a fermented liquid;

[0041] The fermentation culture liquid includes: fructose syrup 18-22g / L, sucrose 18-22g / L, vitamin B1 0.008-0.012g / L, magnesium sulfate 0.6-0.8g / L, potassium dihydrogen phosphate 1.2-1.6g / L L, peptone 0.8-1.2g / L, silkworm chrysalis powder 6-10g / L and soybean oil 0.8-1.2ml / L;

[0042] Preferably, the fermentation broth also includes 4-9g / L mannitol and 0.01-0.05g / L arginine;

[0043] S12, inactivating the fermented liquid obtained in step S11, filtering, and concentrating to obtain an Armillaria concentrated liquid;

[0044] The inactivation is as follows: after the fermentation is terminated, the temperature in t...

experiment example 1

[0068] 1. Extraction and determination of polysaccharides in fermentation broth

[0069] Take a certain volume of fermentation broth and centrifuge at 3000r / min for 10min, take 20mL of the supernatant and concentrate it to 10mL at 50°C, add 3 times the volume of ethanol to precipitate for 12h, centrifuge at 3000r / min for 15min, wash the precipitate with 75% ethanol Centrifuge until there is no reducing sugar reaction, and dissolve the precipitate fully with hot water at 60°C, centrifuge to remove the precipitate, and dilute the supernatant to a 100mL volumetric flask. The content of polysaccharides was determined by the phenol-sulfuric acid method.

[0070] 2. Measurement of the dry weight of mycelia in the fermentation broth

[0071] Filter the fermented product with two layers of gauze, take the mycelium and put it in air-drying at 45°C to constant weight, and convert it into the dry weight of mycelium per liter of fermentation broth.

[0072] The results are shown in Tabl...

experiment example 2

[0076] Experimental Example 2 Functional Evaluation of Lowering Blood Sugar

[0077] db / db diabetic mice: 5 weeks old, male, purchased from Institute of Model Animals, Nanjing University. Measure body weight, random blood sugar, remove rats with abnormal body weight and abnormal blood sugar, and randomly distribute them into 12 groups, 8 in each group; the Naoxinshu oral liquid prepared in Examples 1-7 and Comparative Examples 1-4, respectively, according to 1.08g The dose of / kg / d was administered to db / db diabetic mice by intragastric administration, once a day, for 4 consecutive weeks, and the model control group was given the same dose of normal saline. Fasting blood glucose was monitored weekly, that is, the night before the blood glucose measurement, the mice were fasted, and blood was taken from the tail vein for detection the next day. When measuring blood sugar, treat the mice gently, and try not to make the mice nervous, so as not to affect the accuracy. When measur...

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Abstract

The invention discloses a Naoxinshu oral liquid and a preparation method thereof. The preparation method comprises the following steps: S1, preparation of Armillaria concentrated solution: S11, inoculating Armillaria strains into fermentation culture liquid, fermenting and culturing , to obtain a fermented liquid; S12, inactivate the fermented liquid obtained in step S11, filter and concentrate to obtain a concentrated liquid of Armillaria armillaria; S2, heat and refine the honey, filter, let it cool, and mix it evenly with the ground royal jelly, Sieve, then add Armillaria concentrated solution prepared in step S1, sodium benzoate aqueous solution and vanilla essence ethanol solution in sequence, mix evenly, add purified water to volume, stir evenly, filter, and obtain; at the same time, protect the brain extract prepared by the above method Xinshu Oral Liquid. The invention has short fermentation period, good bacterial growth, high polysaccharide content and stable quality, improves the drug efficacy and quality stability of the product, can meet clinical needs, and the prepared product has better hypoglycemic effect.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and in particular relates to a Chinese medicine preparation Naoxinshu oral liquid which has the functions of nourishing and strengthening, calming the nerves and improving cardiovascular and cerebrovascular diseases and a preparation method thereof. Background technique [0002] Naoxinshu Oral Liquid is a nourishing, strong, calming and calming Chinese patent medicine preparation on the market. It is used for weak body, restlessness, insomnia and dreaminess, neurasthenia, headache and dizziness belonging to the above syndromes. At present, there are 110 production enterprises, involving 110 approval numbers. Its 1000ml prescription volume formula is as follows: [0003] [0004] Its preparation method is as follows: [0005] (1) Armillaria concentrated solution process: [0006] Put 60% of the volume of the culture medium in the fermenter, heat and steriliz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07A61K9/08A61K47/12A61P9/10A61P9/00A61P25/20A61P1/08A61K35/644
CPCA61K9/0095A61K35/644A61K36/07A61K47/12A61P1/08A61P9/00A61P9/10A61P25/20A61K2300/00
Inventor 梁烽焱黄信张坤张永谦程燕梁志军
Owner GUANGDONG YILI LUODING PHARMA
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