Application of dendrobium polysaccharide in preparation of drugs for preventing and/or treating benign prostatic hyperplasia

A technique for benign prostatic hyperplasia and dendrobium polysaccharides, applied in the biological field, to achieve the effects of high polysaccharide yield, low production cost, and high abundance

Active Publication Date: 2021-07-27
中科众康(广东)生物科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the water-soluble polysaccharides of Dendrobium officinale have preventive and recovery effects on benign prostatic hyperplasia, whether its adverse effects on spermatogenesis and function caused by benign prostatic hyperplasia, and whether the polysaccharides of Dendrobium candidum can maintain the stability of the prostate tissue physiological environment and improve its antioxidant capacity. However, it is still blank whether the prevention and adjuvant treatment of benign prostatic hyperplasia have medicinal effects

Method used

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  • Application of dendrobium polysaccharide in preparation of drugs for preventing and/or treating benign prostatic hyperplasia
  • Application of dendrobium polysaccharide in preparation of drugs for preventing and/or treating benign prostatic hyperplasia
  • Application of dendrobium polysaccharide in preparation of drugs for preventing and/or treating benign prostatic hyperplasia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Extraction of Tiepi Dendrobium

[0056] 1. Preparation of Dendrobium polysaccharide water extract

[0057] Weigh 4G Iron Dendrobium powder (purchased in the Guangdong Academy of Agricultural Sciences), 10x volume of distilled water, fully dissolved, water bath at 90 ° C water bath 100min, the mixture is stirred every 30 minutes, gauze is filtered filtrate. Repeat the above process 3 times, combined filtrate, was concentrated with a cryogenic dryer, add 4x volume anhydrous ethanol, 4 ° C for 12 h and deprecated from the experience, and then purified by 80% ethanol 2 to 3 times, 40 ° C Dry the dendrobium crude polysaccharide powder.

[0058] 2. Determination of polysaccharide content

[0059] 100 mg of anhydrous glucose was weighed, and it was placed in a dry 100 mL volumetric flask, and the distilled water was dissolved, and the distilled water to the scale was added to the scale using the dropper, which was obtained to obtain a glucose standard solution. 80% (w / ...

Embodiment 2

[0061] Example 2 Animal Experiment

[0062] Solution formulation

[0063] (1) Propionic acid testosterone injection (25 g / L), purchased from Ningbo second hormone factory (batch number 110251054).

[0064] (2) Propopionic testosterone injection applications: Testosterone injection of the propionic acid was diluted with soybean oil to 0.625 mg / ml, as a mouse skin injection.

[0065] (3) Preparation of Tilder Dendrobium Polysaccharide Solution:

[0066] The iron dendrobium-coated crude polysaccharide powder obtained in Example 1 was dissolved in 100 mL of distilled water, shake well, and made 10 mg / ml of iron dendrobial crude polysaccharide solution.

[0067] 2. Animal Experimental Steps

[0068] From the Guangdong Experimental Animal Center, 5 weeks old, healthy and male Kunming mice, with a weight of 25 ± 2g, and adapt to the start of experimentation after 1 week. The mice were raised in accordance with the national experimental animal feeding standards, room temperature 22 ...

Embodiment 3

[0078] Example 3 Detection of mouse precursor and testicular coefficient

[0079] The separated mouse precursor and testicular weigh separately, calculate the prostate coefficient and the testicular coefficient, as follows:

[0080] Organ index = organ wet weight mg / mouse weight g

[0081] The effect of 1DOP on the prostate coefficient of the mouse (unit: mg / g)

[0082]

[0083]

[0084] Effect of Table 2DOP on the syffice coefficient of mouse (unit: mg / g)

[0085]

[0086] Data n = 10. "A" indicates a significant difference in the blank control group of the same processing time (P 21 The propionic acid testosterone group has significant differences (P 21 Testosterone + Iron Dendrobium Sugar Polysaccharide (P <0.05).

[0087] Depend on figure 1 , figure 2 Table 1, Table 2, Table 2,, compared with the same period, Tiki dendrobium polysaccharide was treated separately from 21 days and 35 days, and there was no significant change in the prostate coefficient of mice (P> 0....

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Abstract

The invention discloses the application of dendrobium polysaccharides in the preparation of drugs for preventing and / or treating hyperplasia of the prostate gland. The dendrobium polysaccharide has obvious therapeutic effects, no toxicity and side effects, can improve the antioxidant capacity of the prostate and testis, restore the normal expression of key genes for steroid synthesis in Leydig cells of benign prostatic hyperplasia, and inhibit 5α-reductase in benign prostatic hyperplasia tissue Gene expression, thereby effectively treating benign prostatic hyperplasia; especially benign prostatic hyperplasia caused by exogenous androgen. The Dendrobium polysaccharide of the present invention has wide sources of raw materials, simple extraction and purification method, high sugar content of the obtained Dendrobium polysaccharide, is suitable for large-scale production and popularization, and has good performance in the preparation of drugs for preventing and / or treating prostatic hyperplasia and clinical application. prospect.

Description

Technical field [0001] The present invention belongs to the basis of biological technology, and in particular, the application of water-soluble polysaccharide in Dendrobium is prepared and / or treats prostate hyperplasia. Background technique [0002] Beneficial Prostate Hyperplasia (BPH) is the most common degenerative disease in middle-aged and elderly men. Among them, the prevalence of men over 40 years old is about 50%, and men over 60 years old have almost all of them have different degrees of prostate hyperplasia. There are many sources of pathogenesis, such as dihydro testosterone, male and female hormone associations say, but its pathogenesis is not fully understood. It is now recognized that male hormone plays an important role in the pathogenesis of BPH. Androgen Testosterone is a steroid hepormon, synthesized by male testicles or female ovaries, which has an important role in the occurrence and function of men, and excessive testosterone in the blood inhibits the form...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P13/08
CPCA61K31/715
Inventor 芦春斌仇平乐李春梦姚炜炜朱倍倍
Owner 中科众康(广东)生物科技研究院
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