Method for extracting polysaccharide from root of pungent litse fruit

A technology of wood ginger and polysaccharides, applied in the field of polysaccharide extraction, to achieve the effects of shortening extraction time, increasing yield, and low energy consumption

Inactive Publication Date: 2013-08-14
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no domestic patent related to the industrial extraction method of polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Grind the root of Caeberia japonica, take 1kg, add water to infiltrate, use ultra-high pressure method to extract, control particle size to be 60 mesh, solid-liquid volume ratio is 1:10, pressure is 400MPa, hold pressure for 5min, repeat 2 times, combine extracts at 50 Add protease to 10 activity units / L at ℃, enzymatically hydrolyze for 4 hours, filter the enzymolyzed solution with 6000 hollow cellulose membrane ultrafiltration, collect the permeate and concentrate it in vacuum below 85℃ until it is concentrated to 1 / 3 of the original volume , add 95% ethanol to the concentrated solution, stir, let it stand for precipitation, use a centrifuge to separate, and the centrifuge speed is 3000r / min to obtain the precipitate, put it in a vacuum drying box for vacuum drying, and the drying temperature is 75°C to obtain the root Polysaccharide 55g, content 71%.

Embodiment 2

[0019] Grind the roots of Caeberia japonica, take 1kg, add water to infiltrate, and extract by ultra-high pressure method, the particle size is controlled to be 60 mesh, the solid-liquid volume ratio is 1:18, the pressure is 400MPa, and the pressure is kept for 3min. Repeat 3 times, and the combined extracts are mixed at 50 Add protease to 20 activity units / L at ℃, enzymatically hydrolyze for 4 hours, filter the enzymolyzed solution with a 10000 hollow cellulose membrane ultrafiltration, collect the permeate and concentrate it in vacuum below 85℃ until it is concentrated to 1 / 3 of the original volume , add 95% ethanol to the concentrated solution, stir, let it stand for precipitation, use a centrifuge to separate, and the centrifuge speed is 3000r / min to obtain the precipitate, put it in a vacuum drying box for vacuum drying, and the drying temperature is 75°C to obtain the root Polysaccharide 49g, content 73%.

Embodiment 3

[0021] Grind the roots of Caeberia japonica, take 1kg, add water to infiltrate, and extract by ultra-high pressure method, the particle size is controlled to be 60 mesh, the solid-liquid volume ratio is 1:15, the pressure is 400MPa, the pressure is maintained for 4min, repeat 3 times, and the combined extracts are mixed at 50 Add protease to 20 activity units / L at ℃, enzymatically hydrolyze for 4 hours, filter the enzymolyzed solution with a 10000 hollow cellulose membrane ultrafiltration, collect the permeate and concentrate it in vacuum below 85℃ until it is concentrated to 1 / 3 of the original volume , add 95% ethanol to the concentrated solution, stir, let it stand for precipitation, use a centrifuge to separate, and the centrifuge speed is 3000r / min to obtain the precipitate, put it in a vacuum drying box for vacuum drying, and the drying temperature is 75°C to obtain the root Polysaccharide 43g, content 70%.

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Abstract

The invention discloses a method for extracting polysaccharide from the root of pungent litse fruit and belongs to the field of biotechnology. The method comprises the following steps: wetting the root of the pungent litse fruit with water; performing extraction twice or three time by using a super high-pressure method under the conditions that particle diameter is 60 meshes, the solid-liquid volume ratio is 1:10-1:18 and the pressure is 400MPa, and the pressure is kept for 3 to 5 minutes; performing enzymolysis on an extracting solution by using protease; performing ultrafiltration on an enzymatic filtering solution by using an ultrafiltration membrane; collecting a permeating solution and concentrating by using the ultrafiltration membrane; collecting a concentrate and precipitating with alcohol; and performing vacuum drying, thereby obtaining the polysaccharide from the root of the pungent litse fruit. The method is simple to operate, high in extracting ratio, low in energy consumption, free of pollution and easy for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting polysaccharides from the root of cayenne japonica. Background technique [0002] Mujiangzi, also known as Muxiangzi, is mainly distributed in the provinces and regions south of the Yangtze River in my country until Tibet. Nature and flavor are pungent, slightly bitter, fragrant, non-toxic. It blooms from November to April of the next year, and the branches and leaves are fragrant. The root bark and leaves are used medicinally. Mujiangzi has the effects of warming the kidney and invigorating the stomach, promoting qi and dispelling stagnation, and is used to treat stomach pain, vomiting, and unknown swelling and toxin embolism. [0003] Studies in recent years have shown that plant polysaccharides not only have unique effects in enhancing the body's immunity, anti-tumor, anti-virus, anti-oxidation, anti-aging, lowering blood sugar, lowering blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘东锋万冬梅
Owner NANJING ZELANG MEDICAL TECH
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