A kind of preparation and purification method of high antioxidant activity Grifola frondosa polysaccharide

A technology of Grifola frondosa polysaccharides and oxidation activity, applied in biochemical equipment and methods, methods based on microorganisms, using added compounds to stimulate growth, etc., can solve problems such as unsatisfactory market applications and production limitations of acidic polysaccharide components , to achieve the effect of improving in vitro antioxidant activity, safety, non-toxic stability, and increasing yield

Active Publication Date: 2022-07-26
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The efficacy of Grifola frondosa has been widely recognized, but the production of acidic polysaccharide components of Grifola frondosa limits the application of the market and cannot meet the growing demand of people

Method used

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  • A kind of preparation and purification method of high antioxidant activity Grifola frondosa polysaccharide
  • A kind of preparation and purification method of high antioxidant activity Grifola frondosa polysaccharide
  • A kind of preparation and purification method of high antioxidant activity Grifola frondosa polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 (control group)

[0065] Grifola frondosa seed culture: Cut the Grifola frondosa grown on PDA plates into 1cm 2 Take 3 to 5 small pieces of the same size and inoculate them into the seed medium of the conical flask with the liquid volume of 100 mL / 250 mL, and cultivate at 160 r / min and 28 °C for 5 days.

[0066] Grifola frondosa fermentation culture: add 150 mL of fermentation medium to a 500 mL conical flask, the inoculum volume is 15 mL of seed liquid, and cultivate at 160 r / min at 28 °C for 8 days.

[0067] Each L of the seed medium contains potato 200g, glucose 20g, peptone 5g, potassium dihydrogen phosphate 2g, magnesium sulfate heptahydrate 1g, vitamin B 1 0.02g, pH natural. Each L of the fermentation medium contains 22 g of glucose, 3 g of peptone, 1.2 g of potassium dihydrogen phosphate, 0.8 g of magnesium sulfate heptahydrate, and vitamin B 1 0.12g, pH natural.

[0068] The extraction method and determination method of Grifola frondosa polysacchar...

Embodiment 2

[0071]Substratum and cultivation method are the same as in Example 1, the difference is that the 0-2d of Grifola frondosa fermented culture, add exogenous additive farnesol 0-0.2mmol / L, add the Grifola frondosa fermented liquid after the addition of farnesol and continue to ferment to 8d. After the fermentation, the mycelium of Grifola frondosa was obtained and the polysaccharide in the fermentation broth was extracted. Wherein, the extraction method and determination method of the polysaccharide in the fermentation broth of Grifola frondosa are the same as those in Example 1, and the polysaccharide content in the fermentation broth of Grifola frondosa was determined to be (0.8371±0.0532) g / L, which was increased by 66% compared with the control group. like figure 2 shown.

Embodiment 3

[0073] The culture medium and the culture method are the same as in Example 1, except that the 0-2 d of the fermentation culture of Grifola frondosa, the addition of an exogenous additive farnesol 0.2-0.4 mmol / L, and the fermentation broth of the Grifola frondosa after the addition of the farnesol continues to ferment to 8d. After the fermentation, the mycelium of Grifola frondosa was obtained and the polysaccharide in the fermentation broth was extracted. Wherein, the extraction method and determination method of the polysaccharide in the fermentation broth of Grifola frondosa are the same as those in Example 1, and the polysaccharide content in the fermentation broth of Grifola frondosa was determined to be (1.0058±0.0128) g / L, which was increased by 98% compared to the control group. like image 3 shown.

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Abstract

The invention discloses a preparation and purification method of Grifola frondosa polysaccharide with high antioxidant activity in vitro. In the method of the invention, by adding farnesol in the liquid fermentation process of Grifola frondosa mycelium, the polysaccharide yield of the fermentation broth of Grifola frondosa is changed, the yield of acidic polysaccharide components produced by the fermentation of Grifola frondosa is remarkably increased, and the original polysaccharide component is not increased. On the basis of the fermentation cycle, the polysaccharide yield of the fermentation broth of Grifola frondosa was significantly improved, with a maximum increase of 149%, and the content of acidic polysaccharides after separation and purification increased from 7.4% to 58.2%. Compared with the control group, the oxidative activity was significantly improved, and the exogenous additive farnesol was safe, non-toxic and highly stable, thus laying the foundation for the industrial production of polysaccharides in the fermentation broth of Grifola frondosa.

Description

technical field [0001] The invention belongs to the technical field of Grifola frondosa polysaccharide extraction, in particular to a preparation and purification method of Grifola frondosa polysaccharide with high antioxidant activity. Background technique [0002] Grifola frondosa polysaccharide is the main bioactive component of Grifola frondosa fermentation broth. A large number of pharmacological and pharmacological studies have proved that the polysaccharide of the fermentation broth of Grifola frondosa has good effects in anti-tumor, anti-HIV virus and prevention and treatment of diabetes, hypertension, hyperlipidemia, obesity, Alzheimer's disease, etc. Lentinan, Yunzhi polysaccharide and Schizophyllan polysaccharide in clinical application. In addition, Grifola frondosa polysaccharides have many active functions that are beneficial to the human body. It can improve the body's ability to tolerate hypoxia, enhance the body's immunity, scavenge free radicals, fight ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12N1/38C12N1/14C08B37/00C12R1/645
CPCC12P19/04C12N1/38C12N1/14C08B37/0003
Inventor 韩培培王晓丽陈旎李健张乐乐贾士儒钟成谭之磊
Owner TIANJIN UNIV OF SCI & TECH
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