Grifola frondosa strain produced through rice bran and wheat bran complete feed liquid fermentation

Abstract

The invention belongs to the technical field of microbe application and good bioscience and discloses a polysaccharide grifola strain produced through a rice bran and wheat bran complete feed liquid culture medium. grifola Grifola sp.JSU1301 is preserved in the China Typical Model Cultivation Center (CCTCC) in June 25th, 2013, and is numbered CCTCC NO: M 2013286 and named a grifola Grifola sp.CCTCC NO: M 2013286. According to the invention, a ultraviolet mutagenesis method is described with figures; a new strain capable of highly producing polysaccharide by starting with Grifola sp.CCTCC NO: M 2011113; the new strain quickly ferments and highly produces polysaccharide in the rice bran and wheat bran complete feed liquid culture medium; the mycelial dry weight and the mycelial polysaccharide productivity of a shake flask are increased by 16.8% and 8.57% compared with those of a starting strain, and those of fermentation in a tank are improved by 30.9% and 29.7%, which reach the highest level at present.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a new mutagenic strain of a grifola frondosa strain which is suitable for growing in a compound liquid medium of rice bran and bran whole materials and high-yields a frondosa frondosa polysaccharide. Background technique [0002] Since Chihara et al. (references: Chihara G, Maeda Y, Hamuro J, et al. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (Berk.) sing [J]. Nature, 1969, 222 (5194): 687- 688.) published a paper on "Nature" in 1969, revealing that lentinan has anti-tumor activity, more than 200 kinds of medicinal fungi with anti-tumor activity have been found, some of which are edible fungi, such as Grifola frondosa, Hericium erinaceus, Ganoderma lucidum, etc. The mycelium, fruiting bodies, sclerotia or spores of these edible fungi can produce various active ingredients such as amino acids, proteins, vitamins, polysaccharides,...

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Problems solved by technology

[0007] In 2011, Li Yongzhuan, a postgraduate student under the guidance of Liu Weimin, tried to solve the problem of liquid fermentation and utilization of rice bran and bran. He experimented with ultraviolet mutagenesis starting from Grifola sp. The rice bran and bran whole material culture medium (Master's thesis p17 rice bran and bran screening medium added agar) was solidified with agar. During the test, the mutagenic effect of the strains was not significant, so the whole rice bran and bran material could not be used. Liquid shake flask fermentation was used to evaluate the mutagenic effect, and the fermentation medium was used to filter rice bran bran juice for fermentation evaluation (Master's thesis p17 fermentation medium, similar to the experiments of Zhang Jian and Guo Chunmei), and the rice bran bran juice was taken used old road

[0010] One of the key problems of the present invention is to obtain the Grifola frondosa bacterial strain suitable for growing on the rice bran whole material liquid medium, because the Grifola frondosa bacterial strain currently owned by the inventor can be grown on the rice bran bran medium for filtering the juice growth, but it cannot grow well on rice bran whole material liquid medium, so it is necessary to carry out mutagenesis screening on existing strains and make a breakthrough

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