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Strain used for fermenting rice bran and wheat bran extracts for producing grifolan

A technology of Grifola frondosa polysaccharide and extract, applied in the directions of microorganism-based methods, fermentation, microorganisms, etc.

Inactive Publication Date: 2012-12-12
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a suitable strain of Grifola frondosa can be screened out, a breakthrough may be made in research and production

Method used

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  • Strain used for fermenting rice bran and wheat bran extracts for producing grifolan
  • Strain used for fermenting rice bran and wheat bran extracts for producing grifolan
  • Strain used for fermenting rice bran and wheat bran extracts for producing grifolan

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Example 1 Laser mutagenesis of Grifola frondosa protoplasts

[0054] a. Preparation of osmotic pressure stabilizer

[0055] 0.6mol / L of mannitol.

[0056] b. Preparation of enzymatic solution

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Abstract

The invention belongs to the fields of microbe application technologies and food biotechnologies, and discloses a strain used for fermenting rice bran and wheat bran extracts for producing grifolan. The grifola frondosa strain is collected in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan, China on Aprial 7th, 2011, and has a strain collection number of CCTCC No: M2011113. The name of the strain is Grifolasp. JSU10-2. According to the invention, through protoplast laser mutation, the strain with high yield of mycelium polysaccharide produced from cheap raw materials is obtained. When the strain and an original strain are respectively used in liquid fermentation of a rice bran and wheat bran composite culture medium, the dry weight and polysaccharide of the mutant strain are respectively increased by 31.7% and 32.6% compared with those of the original strain.

Description

technical field [0001] The invention relates to the technical field of food microbiology application, in particular to a new grifola frondosa mutagenic strain suitable for growing in a compound medium of rice bran and bran extract and high-yielding the frondosa frondosa polysaccharide. Background technique [0002] Scientific research at home and abroad and the domestic market have shown that edible fungus polysaccharides can enhance human immunity and assist in the treatment of tumors and other diseases, and have become an auxiliary product for tumor biotherapy. Domestic medicinal products made from the fruiting bodies of edible fungi include Ganoderma lucidum capsules from Wuhu, Anhui, and Grifola frondosa capsules from Qingyuan, Zhejiang. Non-pharmaceutical health products made from edible fungus extracts are also sold. Internationally, New Zealand produces "GanoPoly" polysaccharide extract series products, which are sold to the United States, Australia, China (including ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 刘伟民郭春梅张建徐立平顾慧敏任晓锋李永转张志才崔凤杰赵杰文沈国栋
Owner JIANGSU UNIV
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