Preparation method of carboxymethyl pachyman

A technology of carboxymethylpachycocos and polysaccharides, which is applied in the field of preparation of carboxymethylparia cocos polysaccharides, can solve the problems of chemical agent residues, etc., and achieve the effect of solving chemical agent residues

Inactive Publication Date: 2014-01-01
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical modification method of pachyphyllin has the problem of chemical agent residue no matter the solvent method or the water method is used to prepare carboxymethylpachyran.

Method used

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  • Preparation method of carboxymethyl pachyman

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preparation example Construction

[0018] The preparation of strain activation medium is calculated by mass percentage: glucose 2.0%, yeast extract 0.2%, peptone 0.2%, K 2 HPO 4 0.1%, KH 2 PO 4 0.046%, MgSO 4 ·7H 2 O0.05%, agar 2.0%, the rest is water, and the pH value is in the natural state.

[0019] The preparation of liquid seed medium is calculated by mass percentage: glucose 2.0%, yeast extract 0.5%, peptone 0.4%, K 2 HPO 4 0.1%, KH 2 PO 4 0.046%, MgSO 4 ·7H 2 O0.05%, the rest is water, and the pH is adjusted to 5.0 with acid and alkali.

Embodiment 1

[0021] 1) Activation of bacteria: stir up a piece of bacteria about 1.0cm from the slope 2 Bacterial blocks are inserted into the center of the plate with activated medium, cultured at a constant temperature of 26°C for 4 days, and the mycelium is evenly covered with the plate, which is used as a solid strain for standby;

[0022] 2) Preparation of seed solution: Fill 60mL liquid seed medium into a 250mL conical flask, use a hole puncher to inoculate the hyphae growing on the outside of the plate into the conical flask, inoculate 4 pieces of bacteria with a diameter of 8mm in each flask, and cultivate The temperature was 26°C, and the shaking flask rotation speed was 150r / min, and the shaking flask culture was carried out for 7 days, and it was used as a liquid strain for standby;

[0023] 3) Fermentation culture: transfer 6% of the medium volume liquid seeds to the shake flask fermentation medium (fermentation medium: glucose 3.0%, carboxymethyl cellulose 0.5%, yeast extract ...

Embodiment 2

[0027] 1) Activation of bacteria: stir up a piece of bacteria about 1.0cm from the slope 2 Bacterial blocks are inserted into the center of the plate with activated medium, cultured at a constant temperature of 26°C for 4 days, and the mycelium is evenly covered with the plate, which is used as a solid strain for standby;

[0028] 2) Preparation of seed solution: Fill 60mL liquid seed medium into a 250mL conical flask, use a hole puncher to inoculate the hyphae growing on the outside of the plate into the conical flask, inoculate 4 pieces of bacteria with a diameter of 8mm in each flask, and cultivate The temperature was 26°C, and the shaking flask rotation speed was 150r / min, and the shaking flask culture was carried out for 7 days, and it was used as a liquid strain for standby;

[0029] 3) Fermentation culture: transfer 6% of the medium volume liquid seeds to the shake flask fermentation medium (fermentation medium: glucose 2.5%, carboxymethyl cellulose 1.0%, yeast extract ...

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Abstract

The invention discloses a preparation method of carboxymethyl pachyman, and particularly provides a method for fermentation preparation of carboxymethyl pachyman by regulating the variety and proportion of a carbon source and a nitrogen source in a Tuckahoe culture medium. Consisting of the processes of Tuckahoe strain activation, seed solution preparation, fermentation cultivation and carboxymethyl pachyman extraction, the preparation method is characterized in that in the process of fermentation cultivation, the fermentation culture medium comprises the following components by weight percentage: the carbon source (2.0%-4.5% of glucose, 0.1%-4.5% of carboxymethyl cellulose), the nitrogen source (0.45%-0.65% of yeast extract and 0.35%-0.55% of peptone), 0.1% of K2HPO4, 0.046% of KH2PO4, 0.05% of MgSO4.7H2O, and the balance water. The method provided in the invention can directly obtain carboxymethyl pachyman with certain degree of substitution and no chemical residue, and the carboxymethyl pachyman can be widely used in medicine, food and other fields.

Description

technical field [0001] The invention relates to a preparation method of polysaccharides, in particular to a preparation method of carboxymethyl pachymane. Background technique [0002] Poria cocos sclerotia is the main source of raw material for Poria cocos processing and utilization or extraction to prepare Poria cocos polysaccharide at present. But the production period of the Poria cocos sclerotia produced by the traditional Poria cocos cultivation method is long, the output is not high, and the consumption of pine wood is large. Producing Poria cocos mycelium with liquid culture technology, extracting active substances such as polysaccharides from Poria cocos mycelium and fermentation liquid can better solve the replacement problem of artificially planted Poria cocos, save a lot of wood, and contribute to my country's resources and biodiversity. Sexual protection is of great significance. [0003] The traditional process of preparing carboxymethylpachyan (CMP) generally...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 胡国元陈默李伟伟刘涛毛军军
Owner WUHAN INSTITUTE OF TECHNOLOGY
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